A simple and static preservation system for shipping retinal pigment epithelium cell sheets

Abstract

433 Effect of the small compound TD -198946 on glycosaminoglycan synthesis and transforming growth factor β 3-associated chondrogenesis of human synovium-derived stem cells in vitro A simple and static preservation system for shipping retinal pigment epithelium cell sheets K. Hori, J. Kuwabara, Y. Tanaka, M. Nishida, N. Koide and M. Takahashi 459 DNA methyltransferases inhibitors effectively induce gene expression changes suggestive of cardiomyogenic differentiation of human amniotic fluid-derived mesenchymal stem cells via chromatin remodeling M. Gasiūnienė, A. Zentelytė, B. Wojtas, S. Baronaitė, N. Krasovskaja, J. Savickienė, B. Gielniewski, B. Kaminska, A. Utkus and R. Navakauskienė 469 Differentiation of the human liver progenitor cell line (HepaRG) on a microfluidic-based biochip M. Jang, A. Kleber, T. Ruckelshausen, R. Betzholz and A. Manz 482 A magnetic three-dimensional levitated primary cell culture system for the development of secretory salivary gland-like organoids The cover image is based on the Research Article A simple and static preservation system for shipping retinal pigment epithelium cell sheets by Kanji Hori et al., https://doi.org/10.1002/term.2805. © iP-TEC® Premier BOX-V8.5/ iP-TEC® Temperature Stabilizer-24. Reproduced with permission by

Temporary Storage of the Human Nasal Tissue and Cell Sheet for Wound Repair

“…Because calcium ions are not present in saline, calcium dependent cell–cell adhesion was not retained in cell sheets preserved in saline ( Figure 2D ). In other studies, a retinal pigment cell sheet was preserved for 5 h ( Hori et al, 2019 ) and an oral mucosal cell sheet was preserved for 12 h ( Oie et al, 2014 ). Therefore, cell sheet quality can be maintained in HBSS for 2 days, which is long enough for stability studies and for transport throughout Japan.…”

“…First, we preserved the cell sheets only at room temperature. Hori et al preserved retinal pigment epithelium cell sheets at 37°C ( Hori et al, 2019 ), and Kawazoe et al preserved skin grafts at 4°C ( Kawazoe et al, 2008 ). Therefore, optimization of temperature may be one of the key factors for long-term preservation of cell sheets.…”

Room temperature shipment does not affect the biological activity of pluripotent stem cell-derived retinal organoids

“…Another study demonstrated the successful shipment of femoral head tissue and Achilles tendon at -40˚C for at least 48 hours indicating that the safety and quality of human tissue could be maintained during transportation at during shipment [8]. Hori and colleagues have demonstrated a new technology for shipping RPE tissues at room temperature by using a heat insulating container to minimise the physical and chemical stress on the cells [10]. Additionally, a recent study has developed a new protocol for shipping retinal organoids at 37˚C to facilitate the preclinical studies in animal models and potentially transplantation by maintaining the viability of the tissue and preventing tissue damage [11].…”