Time- and dose-dependent cytotoxicities of ioxitalamate and indigocarmine in human nucleus pulposus cells

Kim, Kyung-Hyun; Kim, Young-Sung; Kuh, Sung Uk; Park, Hyo-Suk; Park, Jeong Yoon; Chin, Dong Kyu; Kh, Kim; Cho, Young-Eun

doi:10.1016/j.spinee.2013.01.019

Cited by 15 publications

(17 citation statements)

References 34 publications

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“…Literature searches were carried out using the keywords “Lumbar discography,” “cell culture,” either individually or combined (\”OR\” and \”AND), in Cochrane Collaboration, the Cochrane Library, Ovid MEDLINE, ProQuest, the National Library of Medicine and PubMed electronic databases, without language and country restrictions. Only five studies with high‐evidential value were found …”

Section: Discussionmentioning

confidence: 99%

“…Kim et al indicated that ioxitalamate, an ionic iodinated contrast medium, was commonly used for discography or percutaneous endoscopic lumbar discectomy (PELD), even though it had side effects such as anaphylactic shock and renal toxicity. They stated that the indigo carmine, a dye with a distinctive blue colour, was commonly used to stain degenerated NP cells during PELD and that it was widely used in spinal surgery.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, studies with a high‐evidential value regarding degenerated nucleus pulposus (NP) tissue have been reported in the literature. However, there have been no published studies displaying the exact results of how the pharmacological agents to be applied in this method would exert an effect on the intact lumbar disc tissue.…”

Section: Introductionmentioning

confidence: 99%

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Are radio‐contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

Karaarslan

Yılmaz

Özbek

et al. 2018

Basic Clin Pharma Tox

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In the literature, there have been no studies showing clear results on how radio-contrast pharmaceuticals would affect intact disc tissue cells. In this context, it was aimed to evaluate the effects of iopromide and gadoxetic acid, frequently used in the discography, on intact lumbar disc tissue in pharmaco-molecular and histopathological level. Primary cell cultures were prepared from the healthy disc tissue of the patients operated in the neurosurgery clinic. Except for the control group, the cultures were incubated with the indicated radio-contrast agents. Cell viability, toxicity and proliferation indices were tested at specific time intervals. The cell viability was quantitatively analysed. It was also visually rechecked under a fluorescence microscope with acridine orange/propidium iodide staining. Simultaneously, cell surface morphology was analysed with an inverted light microscope, while haematoxylin and eosin (H&E) staining methodology was used in the histopathological evaluations. The obtained data were evaluated statistically. Unlike the literature, iopromide or gadoxetic acid did not have any adverse effects on the cell viability, proliferation and toxicity (P < 0.05). Although this study reveals that radio-contrast pharmaceuticals used in the discography, often used in neurosurgical practice, can be safely used, it should be remembered that this study was performed in an in vitro environment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Are radio‐contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

Karaarslan

Yılmaz

Özbek

et al. 2018

Basic Clin Pharma Tox

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“…Further, studies examining the toxicity of contrast substances indicated that ixotitalamate, used in discography or percutaneous endoscopic lumbar discectomy, induced toxicity in the disc nucleus pulposus and not on cartilage cells [28, 29]. …”

Section: Discussionmentioning

confidence: 99%

“…However, as the physiological structure and/or sensitivity may differ from that of humans, it was reported that the results may not be reliable. In similar studies where animal tissues were not used, commercial cell-lines, known to have lost the in vivo phenotypic and genotypic features, were used instead [10, 14, 18, 21, 28, 31]. …”

Section: Discussionmentioning

confidence: 99%

Iopromide- and gadopentetic acid-derived preparates used in MR arthrography may be harmful to chondrocytes

et al. 2017

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BackgroundMagnetic resonance arthrography, a procedure through which contrast agents containing gadolinium and/or iopromide are administered intra-articularly, has become a useful tool in musculoskeletal diagnosis. Nevertheless, despite being considered safe for systemic use, certain tissue toxicities have been identified for both drugs. In this study, the effects of short-term exposure of human primary chondrocyte cell cultures to gadolinium and/or iopromide contrast agents were examined by assaying for stage-specific embryonic antigen-1 (SSEA-1) protein expression (a chondrogenic differentiation marker), cell viability, toxicity, and proliferation.MethodsHuman articular chondrocytes were grown in monolayer culture and were exposed to iopromide and/or gadolinium diethylenetriamine-pentaacetate (Gd-DPT) for 2 and 6 h. Cell cultures with no drug exposure were used as the control group. Cell differentiation status was assessed according to SSEA-1 protein expression. Contrast agent effects on cell viability and proliferation were analyzed using MTT analysis. Further, changes in cell morphology in relation to the control group were evaluated using inverted light microscopy, environmental scanning electron microscopy (ESEM), and 3-tesla magnetic resonance imaging. The obtained data were statistically compared.ResultsWhen compared with the control group, both SSEA-1 protein expression and cell proliferation were lowest in the Gd-DPT group (P = 0.000). There was a statistically significant correlation between SSEA-1 expression and MTT results (rho = 0.351; P = 0.003).ConclusionsNevertheless, the data obtained from in vitro experiments may not directly correspond to clinical applications. However, the mere fact that a drug used solely for diagnostic purposes may repress chondrocyte cell proliferation should be carefully considered by clinicians.

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Prussian Blue Nanoparticles Stabilize SOD1 from Ubiquitination‐Proteasome Degradation to Rescue Intervertebral Disc Degeneration

et al. 2022

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Discography often destroys the hypoxic environment in the intervertebral disc and accelerates intervertebral disc degeneration (IVDD). Therefore, it often fails to meet the requirements for application in clinical practice. This technology mainly increases the reactive oxygen species (ROS) in the IVD. As so, it is particularly critical to develop strategies to avoid this degeneration mechanism. Prussian blue nanoparticles (PBNPs) are found to enhance development under magnetic resonance T1 and have antioxidant enzyme activity. The key results of the present study confirm that PBNPs alleviate intracellular oxidative stress and increase the intracellular activities of antioxidant enzymes, such as superoxide dismutase 1 (SOD1). PBNPs can rescue nucleus pulposus cell degeneration by increasing oxidoreductase system-related mRNA and proteins, especially by stabilizing SOD1 from ubiquitination-proteasome degradation, thus improving the mitochondrial structure to increase antioxidation ability, and finally rescuing ROS-induced IVDD in a rat model. Therefore, it is considered that PBNPs can be a potential antioxidation-protective discography contrast agent.

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Time- and dose-dependent cytotoxicities of ioxitalamate and indigocarmine in human nucleus pulposus cells

Cited by 15 publications

References 34 publications

Are radio‐contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

Are radio‐contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

Iopromide- and gadopentetic acid-derived preparates used in MR arthrography may be harmful to chondrocytes

Prussian Blue Nanoparticles Stabilize SOD1 from Ubiquitination‐Proteasome Degradation to Rescue Intervertebral Disc Degeneration

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