Thyroid Gland in Free Radical-Induced Oxidative Stress

Kale, Mohan K.

doi:10.1007/978-81-322-2035-0_12

Cited by 2 publications

(4 citation statements)

References 75 publications

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“…The 4-hour exposure was selected for short-term exposure studies of the effect of H 2 O 2 on miRNA and mRNA expression profile. The levels of GSH as well as SOD activity were assessed after exposure of the cells to 25 μ M H 2 O 2 for 4 h as markers for oxidative stress status [ 22 , 23 ]. GSH level in H 2 O 2 challenged cells was significantly reduced by 30% (from 361.44 ± 10.01 mg/mg protein in untreated cells to 251.35 ± 39.23 in the treated cells; P < 0.01).…”

Section: Resultsmentioning

confidence: 99%

“…This level of exposure was chosen to mimic H 2 O 2 concentration in PE placentas previously reported by Zhou et al [ 5 ]. Given the reported duplicitous nature of ROS depending on their levels [ 28 ], we evaluated the oxidative stress status of the cells at this exposure level by assessing GSH level and SOD activity in the challenged cells as markers of oxidative stress [ 22 , 23 ]. Both GSH and SOD are of major importance in intracellular redox regulation [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Reduced glutathione (GSH) levels and superoxide dismutase (SOD) activity were assessed in the cell lysates as markers for oxidative stress [ 22 , 23 ]. To determine the level of GSH, an aliquot (0.2 mL) of cell lysate was added to a tube containing 1.7 mL phosphate buffer and 0.1 mL Ellman's reagent; then the absorbance was read at 412 nm within 5 min [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

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Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts

Cross

Tolba

Rondelli

et al. 2015

BioMed Research International

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The relationship between oxidative stress and miRNA changes in placenta as a potential mechanism involved in preeclampsia (PE) is not fully elucidated. We investigated the impact of oxidative stress on miRNAs and mRNA expression profiles of genes associated with PE in villous 3A first trimester trophoblast cells exposed to H2O2 at 12 different concentrations (0-1 mM) for 0.5, 4, 24, and 48 h. Cytotoxicity, determined using the SRB assay, was used to calculate the IC50 of H2O2. RNA was extracted after 4 h exposure to H2O2 for miRNA and gene expression profiling. H2O2 exerted a concentration- and time-dependent cytotoxicity on 3A trophoblast cells. Short-term exposure of 3A cells to low concentration of H2O2 (5% of IC50) significantly altered miRNA profile as evidenced by significant changes in 195 out of 595 evaluable miRNAs. Tool for annotations of microRNAs (TAM) analysis indicated that these altered miRNAs fall into 43 clusters and 34 families, with 41 functions identified. Exposure to H2O2 altered mRNA expression of 22 out of 84 key genes involved in dysregulation of placental development. In conclusion, short-term exposure of villous first trimester trophoblasts to low concentrations of H2O2 significantly alters miRNA profile and expression of genes implicated in placental development.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts

Cross

Tolba

Rondelli

et al. 2015

BioMed Research International

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“…The thyroid gland is a unique endocrine gland that requires hydrogen peroxide for thyroid hormone synthesis. Oxidative reactions in thyroid gland are predisposing for thyroid hormones synthesis correlated with production of ROS particularly H2O2 under physiological conditions (Kale, 2015). Thyroid gland contain a defined H2O2 generating system, this H2O2 is required as a substrate control the thyroperoxidase, the enzyme catalyzes the Iodide-protein binding during the thyroid hormones synthesis (Corvilain et al, 1991).…”

Section: Ne Usmentioning

confidence: 99%

Effects of Lactobacillus acidophilus on Pituitary-thyroid Axis in Growing Rat

Alqayim¹

2015

Adv. Anim. Vet. Sci.

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| Several studies have shown that gut microbiota can activate neural pathways and central nervous system signalling systems via gut-brain axis, accordingly the present study was aimed to investigate the interaction of Lactobacillis acidophillus on the activity of pituitary-thyroid axis in the case addressed after weaning of male rats. The study was carried out on growing male rats. At the day of weaning 12 male kids at age of 30 days were divided into two equal groups and treated as following: 1 st group considered as control receiving 1ml of water, 2 nd -group received 1ml of MRS (5x10 8 CFU) of L. acidophilus. All the experimental animals were administered orally and daily for the next 32 days. Body weights were recorded at the beginning and end of the experiment. Blood and thyroid gland samples were collected for hormonal assay at the end of the experiment. There was significant differences in body weight gain. Treated group showed less body weight gain than control group. TSH and T3 increased significantly while GH, T4, and TFI were increased slightly in treated group. The analysis of the photomicrograph of thyroid tissue in lactobacillus received group showed increase in the number and size of the thyroid follicles, in addition to a visible hyperchromatic of the follicles epithelia. In conclusion, feeding of Lactobacillus acidophilus to rat kids showed tangible changes in the pituitary-thyroid axis activity suggesting a positive interaction between Lactobacillus acidophilus and host gut-brain axis.

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Thyroid Gland in Free Radical-Induced Oxidative Stress

Cited by 2 publications

References 75 publications

Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts

Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts

Effects of Lactobacillus acidophilus on Pituitary-thyroid Axis in Growing Rat

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