Thyroid follicular morphogenesis mechanism: Organ culture of the fetal gland as an experimental approach

Fine structural aspects of follicle-like cavity formation from dispersed porcine thyroid cells cultured in a collagen substrate.

“…Later, however, REMY et al (1980) confirmed the descriptions by HILFER (1964) andTANIZAWA (1966), in reporting that the follicle lumen originates from the intercellular space in the rat thyroid in vivo, and that intracellular cavities result from a TSHinduced acceleration of vesicle coalescence before they reach the contact zones of the plasma membrane (REMY et al, 1983).…”

Inter- and Intracellular luminal formation in porcine thyroid tissues cultured in a collagen substrate.

“…The occurrence of the intracellular lumina has been reported in the organ-cultured thyroid gland (REMY et al, 1983), in cultured open follicles (DENEF et al, 1980;EKHOLM and BJOERKMAN, 1984), and in cultured dispersed cells (EKHOLM and BJOERKMAN, 1984;ROUSSET et al, 1985).…”

“…As to the origin and development of the follicular lumina in vivo, many studies have been performed using mammalian and chick embryos. Two ways have been established: one being that the primitive follicular lumen appears between neighboring two cells (HILFER, 1964;FUJITA and TANIZAWA, 1966;TILE et al, 1977;ISHIMURA and FUJITA, 1979;REMY et al, 1980), the other being that the primitive follicular lumen originates from the fusion of intra-cellular lumina (SHEPARD, 1968;OLIN et al, 1970;CALVERT and PUSTERLA, 1973;REMY et al, 1983).…”

“…As to the mechanism of formation for the intracellular follicular lumen, it is suggested that it is derived from the coalescence of clear vesicles from the Golgi apparatus (REMY et al, 1983;EKHOLM and BJOERKMAN, 1984;ROUSSET et al, 1985).…”

Hollowing or cavitation during follicular lumen formation in the differentiating thyroid of grass snake Natrix natrix L. (Lepidosauria, Serpentes) embryos? An ultrastructural study

“…It cannot be excluded that the intracellular cavities that participate in the process of follicular lumen formation in other vertebrate species under in vitro conditions were formed with the participation of the Golgi apparatus because in many cases the cultured medium contained TSH, which activates thyroglobulin synthesis (Rémy et al, 1977a(Rémy et al, ,b, 1983. Some authors have indicated that the intracellular follicular lumens are probably not a significant step in thyroid morphogenesis but may be the result of a non-physiological increase in Golgi secretion due to organ culture conditions or other circumstances .…”