Fine structural aspects of follicle-like cavity formation from dispersed porcine thyroid cells cultured in a collagen substrate.

Kitajima, Koichi; Yamashita, Keisuke; Fujita, Hisao

doi:10.1679/aohc.50.113

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…From the findings displaying the fusion of the intracellular follicular lumina with each other or the opening of the intrafollicular lumen to the preformed intercellular follicular lumen, it can be easily understood that some intrafollicular lumina are fated to be fused with each other to form an intercellular lumen, while others are fused with the preformed intercellular follicular lumen. By our previous study, it was clear that the formation of intercellular follicular lumina between two layers of the cultured thyroid cells is a major factor in folliculogenesis (KITAJIMA et al, 1987), and the fusion of intracellular follicular lumina is considered to be of minor importance in making a follicular lumen. The intracellular lumina also have the function of helping the expansion of the preformed interfollicualr lumen through fusion with it.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous paper (KITAJIMA et al, 1987), we reported on the formation mechanism of the follicle-like cavity from a monolayer of porcine thyroid cells cultured in a collagen sandwich. In such a case, the folliclelike cavities have been known to appear generally between the cells of the upper layer and those of the lower layer, which were formed by the proliferation and migration of the monolayered cells (KITAJIMA et al, 1987).…”

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confidence: 99%

“…In such a case, the folliclelike cavities have been known to appear generally between the cells of the upper layer and those of the lower layer, which were formed by the proliferation and migration of the monolayered cells (KITAJIMA et al, 1987). In addition, it has become clear that an intracellular follicular lumen sometimes appears in cultured cell cytoplasm, later opening to the intercellular follicular cavity.…”

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Inter- and Intracellular luminal formation in porcine thyroid tissues cultured in a collagen substrate.

Yamashita

Fujita

Kitajima

et al. 1989

Archives of Histology and Cytology

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Inter- and Intracellular luminal formation in porcine thyroid tissues cultured in a collagen substrate.

Yamashita

Fujita

Kitajima

et al. 1989

Archives of Histology and Cytology

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“…However, these follicles lack a basal lamina (Fayet et al, 1982). Without TSH stimulation, thyroid cells can form follicles and a basal lamina if they are cultured in collagen gel (Kitajima et al, 1987;Toda & Sugihara, 1990) or on the surface of a reconstituted basement membrane matrix (Espanet et al, 1992) The mechanism of thyroid cell re-aggregation is still unclear (Toda & Sugihara, 1990;Toda et al, 1993). A basal lamina can also be detected when thyroid follicles are cultured on agarose-coated dishes in medium containing collagen and laminin (Garbi & Wollman, 1982).…”

Section: Introductionmentioning

confidence: 99%

Basal lamina formation by porcine thyroid cells grown in collagen- and laminin-deficient medium

1995

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Porcine thyroid cells isolated by dispase treatment were cultured in either (a) Matrigel, (b) agarose with the addition of different combinations of basic fibroblast growth factor and laminin, or (c) on agarose-coated dishes. The formation of follicles and the presence of a basal lamina was investigated by routine electron microscopy of Araldite-embedded material and by light and electron microscopical immunocytochemical detection of the basal lamina components, laminin and collagen type IV. After 10 days of culture in Matrigel or agarose, a basal lamina-like structure surrounded most follicles. Follicles of cells growing in agarose and overlaid with a medium containing thyrotropin and overlaid with a medium containing thyrotropin and fibroblast growth factor showed a fluorescent band at the basal side of the follicles after immunocytochemical staining with anti-laminin and anti-collagen antibodies. Routine electron microscopy showed that a basal lamina-like structure lined the outside of the follicle. This structure could be subdivided into a lamina lucida and a lamina densa. Electron microscopical immunogold labelling revealed that immunologically detectable laminin was confined to the lamina densa. These findings suggest that even in the absence of basal lamina components in the culture medium, thyroid cells are able to form follicles with a regular basal lamina when they are cultured in a three-dimensional environment.

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Capacities of a Newly Established Thymic Stromal Cell Clone to Express la Antigens and to Produce Interleukin-6, Colony-Stimulating Factor, and Thymic Stroma-Derived T-Cell Growth Factor

Ogata

Matsubara

Takai

et al. 1989

Journal of Leukocyte Biology

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Thymic stromal cell lines, termed MRL104 and MRL28, have been isolated from long-term liquid cultures of thymic stromal cells from MRL/l mice. The capacities of these parental lines and derived clones to express Ia antigens and to produce cytokines involved in T-cell proliferation and/or differentiation were investigated. Parental lines and their clones did not exhibit a typical fibroblastic, macrophage-like, or epithelial appearance in electron as well as phase-contrast micrographs. These thymic stromal cells seemed to differ from established fibroblast lines in that these thymic stromal cells expressed Ia antigens after exposure to interferon-gamma (IFN-gamma), whereas fibroblast lines did not. They also appeared to differ from macrophage cell lines in that they lacked the expression of Mac-1 antigens on their cell surface and produced no detectable level of interleukin-1 (IL1) before or even after exposure to lipopolysaccharide. When these parental lines and its clones were tested for their ability to produce various types of cytokines, it was revealed that they were capable of producing colony-stimulating factor (CSF), IL6, and thymic stroma-derived T cell growth factor (TSTGF), which was recently described, but were unable to generate other lymphokines and IFNs. Thus these cell lines and clones represent unique features in that they have potentials to express Ia antigens and to produce CSF, IL6, and TSTGF. The biological significance for the expression of these features is discussed in the context of intrathymic T-cell maturation and T-cell repertoire selection.

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Fine structural aspects of follicle-like cavity formation from dispersed porcine thyroid cells cultured in a collagen substrate.

Cited by 7 publications

References 29 publications

Inter- and Intracellular luminal formation in porcine thyroid tissues cultured in a collagen substrate.

Inter- and Intracellular luminal formation in porcine thyroid tissues cultured in a collagen substrate.

Basal lamina formation by porcine thyroid cells grown in collagen- and laminin-deficient medium

Capacities of a Newly Established Thymic Stromal Cell Clone to Express la Antigens and to Produce Interleukin-6, Colony-Stimulating Factor, and Thymic Stroma-Derived T-Cell Growth Factor

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