Inter- and Intracellular luminal formation in porcine thyroid tissues cultured in a collagen substrate.

Yamashita, Keisuke; Fujita, Hisao; Kitajima, Koichi; Nishii, Yoshio

doi:10.1679/aohc.52.109

Cited by 8 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several recent studies have demonstrated that many cell activities, including differentiation, can be regulated by mechanical cues such as substrate stiffness [61] , [62] , [63] and dimensionality (2D vs. 3D) [64] . In fact, thyroid epithelial cells cultured in static 3D collagen gels do form follicles with structural polarity [60] , [65] , [66] , presumably because of favorable mechanical and compositional cues. Our next steps will be to translate our findings here to a similar 3D collagen gel system [67] .…”

Section: Discussionmentioning

confidence: 99%

Vibratory stimulation enhances thyroid epithelial cell function

Wagner

Chinnathambi

Titze

et al. 2016

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

The tissues of the body are routinely subjected to various forms of mechanical vibration, the frequency, amplitude, and duration of which can contribute both positively and negatively to human health. The vocal cords, which are in close proximity to the thyroid, may also supply the thyroid with important mechanical signals that modulate hormone production via mechanical vibrations from phonation. In order to explore the possibility that vibrational stimulation from vocalization can enhance thyroid epithelial cell function, FRTL-5 rat thyroid cells were subjected to either chemical stimulation with thyroid stimulating hormone (TSH), mechanical stimulation with physiological vibrations, or a combination of the two, all in a well-characterized, torsional rheometer-bioreactor. The FRTL-5 cells responded to mechanical stimulation with significantly (p<0.05) increased metabolic activity, significantly (p<0.05) increased ROS production, and increased gene expression of thyroglobulin and sodium-iodide symporter compared to un-stimulated controls, and showed an equivalent or greater response than TSH only stimulated cells. Furthermore, the combination of TSH and oscillatory motion produced a greater response than mechanical or chemical stimulation alone. Taken together, these results suggest that mechanical vibrations could provide stimulatory cues that help maintain thyroid function.

show abstract

Section: Discussionmentioning

confidence: 99%

Vibratory stimulation enhances thyroid epithelial cell function

Wagner

Chinnathambi

Titze

et al. 2016

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

show abstract

“…Pseudolumina, which are regarded as a clear-cut sign of differentiation in epithelial gland cells (Kitajima et al, 1987;Yamashita et al, 1989), were only occasionally found in the MCF-7 cells.…”

Section: Mcf-7 Cells Co-cultured Withmentioning

confidence: 99%

Differentiation of breast cancer cells in vitro is promoted by the concurrent influence of myoepithelial cells and relaxin

Bani

Riva²,

Bigazzi³

et al. 1994

Br J Cancer

View full text Add to dashboard Cite

nmaOurpreIious studies showed that relaxin promotes differentiation of MCF-7 breast adenocarcinoma cells. In the current investigation. we aimed to elucidate whether the effect of the hormone is potentiated when When relaxin was added to the co-cultures, the ultrastructural signs of differentiation could be observed in esen more MCF-7 cells and became more pronounced than in the absence of the hormone, judged by the appearance of a clear-cut polarisation of cytoplasmic organelles, an almost continuous coat of apical microvilli and numerous intracellular pseudolumina.

show abstract

“…Collagen gels. Gels were prepared according to Yamashita et al (29). In brief, 8 volumes of a solution of collagen type I (2 to 3 mg/ml) were mixed with 1 volume of l o x culture medium, 1 volume of buffer (200 mmol/l HEPES, 260 mmol/l NaHCO,, 50 rnmol/l NaOH), and I volume of FCS.…”

Section: Culture Conditionsmentioning

confidence: 99%

Pineal Photoreceptor Cells: Photoperiodic Control of Melatonin Production After Cell Dissociation and Culture

Bégay

Falcón

Thibault

et al. 1992

J Neuroendocrinology

View full text Add to dashboard Cite

Trout pineal cells were dissociated using a trypsin-DNase digestion technique. An enriched population of photoreceptor cells was selected from a Percoll gradient centrifugation. The ability of cultured photoreceptor cells (selected or not on a Percoll gradient) to produce melatonin rhythmically was investigated during seven 24 h light/dark cycles. During each cycle, trout pineal photoreceptor cells released low amounts of melatonin during daytime and high amounts during night-time. Under continuous darkness, melatonin release was continually high. The profile of its rhythm and that of the activity of the hydroxyindole-O-methyltransferase-the last enzyme of the melatonin biosynthetic pathway-depended on the substrates and on the culture media used. Some of them appear suitable for short- or long-term culture of photoreceptor cells permitting the study of their neuroendocrine properties.

show abstract

Inter- and Intracellular luminal formation in porcine thyroid tissues cultured in a collagen substrate.

Cited by 8 publications

References 15 publications

Vibratory stimulation enhances thyroid epithelial cell function

Vibratory stimulation enhances thyroid epithelial cell function

Differentiation of breast cancer cells in vitro is promoted by the concurrent influence of myoepithelial cells and relaxin

Pineal Photoreceptor Cells: Photoperiodic Control of Melatonin Production After Cell Dissociation and Culture

Contact Info

Product

Resources

About