Thymic stromal lymphopoietin expression is increased in the horny layer of patients with atopic dermatitis

Sano, Yoichiro; Masuda, Koji; Tamagawa‐Mineoka, Risa; Matsunaka, Hiroshi; Murakami, Yumi; Yamashita, Ryo; Morita, Eishin; Katoh, Norito

doi:10.1111/cei.12021

Cited by 105 publications

(66 citation statements)

References 29 publications

(34 reference statements)

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“…TSLP protein is undetectable in non-lesional skin in healthy individuals and in AD patients [63,71]; however, TSLP is highly expressed in acute and chronic AD lesions [71,97]. In particular, it is overexpressed in the skin stratum corneum and it correlates with severity scoring of AD index and epidermal barrier function, such as stratum corneum hydration and transepidermal water loss [97]. Interestingly, moisturizer application results in reduced skin TSLP levels and reduced AD symptoms and scores [97].…”

Section: Tslp and Atopic Dermatitismentioning

confidence: 99%

“…In particular, it is overexpressed in the skin stratum corneum and it correlates with severity scoring of AD index and epidermal barrier function, such as stratum corneum hydration and transepidermal water loss [97]. Interestingly, moisturizer application results in reduced skin TSLP levels and reduced AD symptoms and scores [97]. In a mouse model, overexpression of skin TSLP was enough to induce a disease phenotype similar to AD [98].…”

Section: Tslp and Atopic Dermatitismentioning

confidence: 99%

See 1 more Smart Citation

The importance of TSLP in allergic disease and its role as a potential therapeutic target

Cianferoni¹,

Spergel

2014

Expert Review of Clinical Immunology

169

116

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Thymic stromal lymphopoietin (TSLP) is an epithelial-derived cytokine similar to IL- 7, whose gene is located on chromosome 5q22.1 and it exerts its biological function through the TSLP-Receptor (TSLP-R). TSLP is expressed primarily by epithelial cells at barrier surfaces such as the skin, gut and lung in response to danger signals. Since it was cloned in 1994, there has been accumulating evidence that TSLP is crucial for the maturation of antigen presenting cells and hematopoietic cells. TSLP genetic variants and its dysregulated expression have been linked to atopic diseases such as atopic dermatitis, asthma, allergic rhinitis and eosinophilic esophagitis.

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Section: Tslp and Atopic Dermatitismentioning

confidence: 99%

Section: Tslp and Atopic Dermatitismentioning

confidence: 99%

The importance of TSLP in allergic disease and its role as a potential therapeutic target

Cianferoni¹,

Spergel

2014

Expert Review of Clinical Immunology

169

116

View full text Add to dashboard Cite

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“…The study was approved by the Ethics Committee of the University of Toyama. The SCORAD index was used to evaluate the severity of AD (11), and the local AD severity score in the involved skin was measured according to the scores for erythema, oedema/papules, oozing/crust formation, excoriation, lichenification and xerosis, each of which was assessed on a four-point scale (12).…”

Section: Subjects and Evaluation Of Clinical Featuresmentioning

confidence: 99%

Macrophage migration inhibitory factor (MIF) in the stratum corneum: a marker of the local severity of atopic dermatitis

Yasuda

Enomoto

Ishiwatari

et al. 2014

Experimental Dermatology

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Different biomarkers are used to evaluate the severity of atopic dermatitis (AD); however, it remains difficult to determine the severity of localized skin lesions. MIF plays an essential role in the pathophysiology of skin inflammation. To establish whether the MIF level in the stratum corneum (SC) serves as a marker of the severity of AD lesions, we examined the SC MIF (scMIF) levels in AD patients. The SC of the cheek, neck and upper arm skin was collected using tape stripping, and the scMIF levels were measured. Consequently, the scMIF levels were found to be significantly higher in the involved skin lesions than the uninvolved areas within the same patient. Moreover, the scMIF levels were significantly correlated with the severity of local skin lesions. These findings suggest that the scMIF level can be used as an effective marker for evaluating the local severity of AD.

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“…The release ofIL-33/TSLPbythe epithelium is an important proximal eventin the induction and maintenance of Th2 inflammation [16, 17], and they are known as initiative key factors of allergic inflammation. TSLP expression level increases in AD patients, which can be used as a biomarker of AD severity and particularly the epidermal barrier status [18]. It may also be a master switch for allergic inflammation at the EC-DC interface [19].…”

Section: Introductionmentioning

confidence: 99%

Calycosin Suppresses Epithelial Derived Initiative Key Factors and Maintains Epithelial Barrier in Allergic Inflammation via TLR4 Mediated NF-κB Pathway

Wang

et al. 2017

Cell Physiol Biochem

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Background/Aims: Calycosin is a bioactive component of Astragali Radix, a Chinese herb for treating allergy. We have previously demonstrated that calycosin effectively inhibited allergic inflammation efficiently. The aim of this study was to explore the mechanism of calycosin on epithelial cells in allergic inflammation. Methods: An initial stage of atopic dermatitis (AD) model in which mice were just sensitized with FITC, was established in vivo and immortalized human keratinocytes (HaCaT cells) were utilized in vitro. Initiative key cytokines, TSLP and IL-33, were measured by ELISA, qPCR, immunofluorescence and Western blot. The junctions in epithelial cells were observed by electron microscopy and tight junctions (TJs) (Occludin and ZO-1) were assessed by Western blot and immunofluorescence. TLR4, MyD88, TAK1, TIRAP and NF-κB were measured by qPCR or Western blot. Results: The results showed that TSLP and IL-33 were inhibited significantly by calycosin in the initial stage of AD model. Simultaneously, calycosin attenuated the separated gap among the epithelial cells and increased the expression of TJs. TSLP/IL-33 and TJs were similarly affected in LPS-stimulated HaCaT cells in vitro. Meanwhile, calycosin not only inhibited the expressions of TLR4, MyD88, TAK1 and TIRAP, but also reduced NF-κB activation in vitro and in vivo. An NF-κB inhibitor enhanced the expressions of TJs and reduced that of TSLP/IL-33 in LPS-stimulated HaCaT cells. Conclusion: These results indicated that calycosin reduced the secretion of TSLP/IL-33 and attenuated the disruption of epithelial TJs by inhibiting TLR4 mediated NF-κB signaling pathway. These findings help to understand the beneficial effects of calycosin on AD, and to develop effective preventive or therapeutic strategies to combat this disease and other epithelial barrier deletion-mediated allergic diseases.

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Thymic stromal lymphopoietin expression is increased in the horny layer of patients with atopic dermatitis

Cited by 105 publications

References 29 publications

The importance of TSLP in allergic disease and its role as a potential therapeutic target

The importance of TSLP in allergic disease and its role as a potential therapeutic target

Macrophage migration inhibitory factor (MIF) in the stratum corneum: a marker of the local severity of atopic dermatitis

Calycosin Suppresses Epithelial Derived Initiative Key Factors and Maintains Epithelial Barrier in Allergic Inflammation via TLR4 Mediated NF-κB Pathway

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