Thrombin Generation Assay and Hemostatic Profile for Elucidating Hypercoagulability in Endogenous Canine Hyperadrenocorticism

Vargas-Mora, Silvia E.; Romão, Felipe Gazza; Santos, Bruna dos; Carvalho, Maria das Graças; Duarte, Rita Carolina Figueiredo; Bernardes, Viviani De Marco; Lopes, Priscila A.; Takahira, Regina Kiomi

doi:10.1016/j.tcam.2021.100581

Cited by 3 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…49 Applications for TG assays in people include investigation of hemorrhagic coagulopathies, monitoring replacement therapy in hemophilia, prediction of venous thromboembolism, and monitoring antithrombotic drugs. 50 Thrombin generation assays have also been validated in dogs, 14 with descriptions of its use in recognizing hypercoagulability in dogs with hyperadrenocorticism, 51 and as a therapeutic monitoring option for anticoagulants. [15][16][17] The utility of TG assays for monitoring the platelet inhibitory effect of clopidogrel in dogs has not been previously investigated however.…”

Section: Discussionmentioning

confidence: 99%

“…12 Thrombin generation (TG) assays quantify the amount of thrombin generated following in vitro activation, thereby providing insight into the cumulative impact of platelet and clotting factor function in an individual patient. Thrombin generation assays have been evaluated in dogs 13,14 and are decreased following heparin and rivaroxaban administration. [15][16][17] Rodent models demonstrate reduced TG following clopidogrel administration as well, 18 but clopidogrel inconsistently attenuates TG in people.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of a thrombin generation assay in dogs administered clopidogrel

Rank

Lynch

Ruterbories

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: The antiplatelet effect of clopidogrel can vary between patients. A modified thromboelastography (TEG) protocol (TEG-Platelet Mapping assay® [TEG-PM]) can be used for clopidogrel monitoring but is not widely available. Thrombin generation (TG) assays could offer a novel alternative. The main objective of this pilot study was to assess TG assay variables (lag time, peak, endogenous thrombin potential [ETP]) in dogs before and after 7 days of clopidogrel administration, and compare with TEG-PM variables (maximum amplitude [MA]-ADP and percentage (%) inhibition). Six healthy mix-breed dogs were enrolled in this pilot study. Blood samples for platelet count, TG assays, and TEG-PM were obtained at two time points, corresponding to baseline, and after 7 days of clopidogrel administration (mean 2.3 +/- 0.3 mg/kg PO q24 hours). Data were then compared with a Student’s t-test. Results There was no significant change in TG assay variables performed on platelet poor plasma after 7 days of clopidogrel administration: lag time (Day 1: 1.8 +/- 0.2 min, Day 7: 1.8 +/- 0.2 min, P = 0.42); Peak (Day 1: 76 +/- 7 nM, Day 7: 72 +/- 10 nM, P = 0.49); and ETP (Day 1: 399 +/- 27 nM*min, Day 7: 392 +/- 32 nM*min; P = 0.49). There were significant changes in TEG MA-ADP (Day 1: 19 +/- 8 mm, Day 7: 9 +/- 6 mm, P = 0.04) and % inhibition (Day 1: 58 +/- 27, Day 7: 99 +/- 0.3, P = 0.02) however over the course of the study. Conclusions Clopidogrel administration did not lead to changes in TG assay variables performed on platelet poor plasma samples, despite concomitant changes in TEG-PM variables consistent with platelet inhibition. Thrombin generation performed on platelet poor plasma does not appear to be a useful antiplatelet monitoring tool in dogs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of a thrombin generation assay in dogs administered clopidogrel

Rank

Lynch

Ruterbories

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

Evaluation of thrombin generation in dogs administered clopidogrel

Rank,

Lynch,

Ruterbories

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

IntroductionThe antiplatelet effect of clopidogrel can vary between patients. A modified thromboelastography (TEG) protocol (TEG-Platelet Mapping assay® [TEG-PM]) can be used for clopidogrel monitoring but is not widely available. Thrombin generation (TG) assays could offer a novel alternative. The main objective of this pilot study was to assess TG assay variables (lag time, peak, endogenous thrombin potential [ETP]) in dogs before and after 7 days of clopidogrel administration and compare with TEG-PM variables (maximum amplitude [MA]-ADP and percentage (%) inhibition).MethodsSix healthy mix-breed dogs were enrolled in this pilot study. Blood samples for platelet count, TG assays, and TEG-PM were obtained at two time points, corresponding to baseline, and after 7 days of clopidogrel administration (mean 2.3 +/− 0.3 mg/kg PO q24 hours). Data were then compared with a Student’s t-test.ResultsThere was no significant change in TG assay variables performed on platelet poor plasma after 7 days of clopidogrel administration: lag time (Day 1: 1.8 +/− 0.2 min, Day 7: 1.8 +/− 0.2 min, p = 0.42); peak (Day 1: 76 +/− 7 nM, Day 7: 72 +/− 10 nM, p = 0.49); and ETP (Day 1: 399 +/− 27 nM*min, Day 7: 392 +/− 32 nM*min; p = 0.49). There were significant changes in TEG MA-ADP (Day 1: 19 +/− 8 mm, Day 7: 9 +/− 6 mm, p = 0.04) and % inhibition (Day 1: 58 +/− 27, Day 7: 99 +/− 0.3, p = 0.02).DiscussionClopidogrel administration did not lead to changes in TG assay variables performed on platelet poor plasma samples, despite concomitant changes in TEG-PM variables consistent with platelet inhibition. Based on this pilot study, thrombin generation performed on platelet poor plasma may not be a useful antiplatelet monitoring tool in dogs.

show abstract

Evaluation of Haemostasis in Dogs Affected by Resectable Malignancy

Bruno

Maurella

Gianella

et al. 2022

Animals

View full text Add to dashboard Cite

Dogs with neoplasm are at risk of thrombosis, yet there is no information on the haemostatic alterations that may result from the surgeries performed to remove neoplastic masses. The aim of this study was to observe the characteristics of haemostasis in dogs undergoing surgery to remove a malignancy by means of rotational thromboelastometry and thrombin generation. Haemostasis was assessed immediately before surgery (T0), 24 h after surgery (T1), and two weeks after surgery (T2). Statistically significant differences were found between the thromboelastometric parameters at T0 vs. T1, with increases observed in MCF, the α angle, and G parameters in the ex-TEM and in-TEM profiles. In the thromboelastometric analysis performed after surgery differences were identified between T1 and T2, with a decrease observed in parameters such as CFT, MCF, the α angle, and G. Between T0 and T2, only a significant decrease in CT was detected in the fib-TEM profile. No differences were found in the comparison among the results obtained via thrombin generation. In dogs with resectable malignancies, the surgeries performed to remove cancer affected coagulation, causing a tendency towards hypercoagulability. The interference with coagulation was transient, and 2 weeks after surgery, the thromboelatometric results returned to those obtained before surgery (T0).

show abstract

Thrombin Generation Assay and Hemostatic Profile for Elucidating Hypercoagulability in Endogenous Canine Hyperadrenocorticism

Cited by 3 publications

References 36 publications

Evaluation of a thrombin generation assay in dogs administered clopidogrel

Evaluation of a thrombin generation assay in dogs administered clopidogrel

Evaluation of thrombin generation in dogs administered clopidogrel

Evaluation of Haemostasis in Dogs Affected by Resectable Malignancy

Contact Info

Product

Resources

About