Threonine-Rich Repeats Increase Fibronectin Binding in the
            <i>Candidaalbicans</i>
            Adhesin Als5p

Rauceo, Jason M.; Armond, Richard De; Otoo, Henry N.; Kahn, Peter C.; Klotz, Stephen A.; Gaur, Nand K.; Lipke, Peter N.

doi:10.1128/ec.00120-06

Cited by 115 publications

(151 citation statements)

References 35 publications

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“…Previous studies also suggested that tandem-repeat-containing domains are essential for stabilizing the correct conformation of the N-terminal ligand-binding domain and contributing positively to the adherence activity mediated by members of the Als family of adhesins (Loza et al, 2004;Rauceo et al, 2006). Our data also demonstrated that the Eap1p Ser/Thr-rich domain containing tandem repeats was able to mediate adhesion to polystyrene and to mammalian epithelial cells directly, in addition to its function of projecting the N-terminal domain into the extracellular environment.…”

Section: Discussionmentioning

confidence: 99%

“…These adhesins, in many cases, also contain serine/threonine-rich tandem repeat domains essential for proper presentation of the Nterminal substrate-binding sites (Frieman et al, 2002;Loza et al, 2004). However, the conserved Als tandem repeats in Als5p facilitate adhesion to fibronectin and greatly increase cell-cell aggregation (Rauceo et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Many insights into the functions of C. albicans adhesins have been derived in C. albicans (Fu et al, 2002;Li et al, 2007;Phan et al, 2007;Staab et al, 1999;Zhao et al, 2005Zhao et al, , 2007. Heterologous expression of C. albicans adhesins in S. cerevisiae has also proven valuable in exploring C. albicans adhesive function (Loza et al, 2004;Rauceo et al, 2006). Eap1p mediates adhesion to many substrates, including synthetic materials, yeast cells and mammalian cells, when expressed in both S. cerevisiae and C. albicans, suggesting that S. cerevisiae may be used as a relevant heterologous system to study the adhesive function of Eap1p in C. albicans.…”

Section: Introductionmentioning

confidence: 99%

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Distinct domains of the Candida albicans adhesin Eap1p mediate cell–cell and cell–substrate interactions

Palecek

2008

Microbiology

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The adhesion of Candida albicans to host tissues contributes to its virulence, and adhesion to tissues or medical devices is a necessary step in biofilm formation. EAP1 encodes a glycosylphosphatidylinositol (GPI)-anchored glucan-cross-linked cell wall protein that mediates adhesion of C. albicans to various materials and cells, and appears to be required for C. albicans biofilm formation in vitro and in vivo. In this study, we demonstrated that the Eap1p N-terminal signal peptide and C-terminal GPI-anchor sequences result in similar protein localization in Saccharomyces cerevisiae and C. albicans. To investigate the contribution of different Eap1p domains to adhesion, we expressed Eap1p domain deletion mutants in non-adherent S. cerevisiae strains. The N-terminal domain mediates yeast cell-cell adhesion and invasive growth. Two Ser/Thr-rich domains containing tandem repeats were required to project the N-terminal region into the extracellular environment and to mediate adhesion to polystyrene. The N-terminal tandem repeat domain mediated adhesion to mammalian epithelial cells and promoted S. cerevisiae pseudohyphal growth. These results suggest a modular structure of Eap1p in which each domain serves multiple, often distinct, functions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distinct domains of the Candida albicans adhesin Eap1p mediate cell–cell and cell–substrate interactions

Palecek

2008

Microbiology

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“…Furthermore, the disruption of INT1 suppressed blastospore adhesion to epithelial cells, hyphal growth, and virulence in mice (Gale et al, 1998). In addition to INT1, agglutinin family proteins, ALA1 and ALS1, and the fibronectin binding protein, FLS5, were also involved in blastospore adhesion (Fu et al, 1998;Rauceo et al, 2006).…”

Section: Animal Pathogenic Fungal Adhesionmentioning

confidence: 99%

The Role of the Extracellular Matrix (ECM) in Phytopathogenic Fungi: A Potential Target for Disease Control

Ikeda¹,

Inoue²,

Kitagawa³

et al. 2012

Plant Pathology

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“…Different cell wall protein adhesion candidates are: The Als (agglutinin-like sequence) family: Till date 8 ALS genes have been recognized ALS 1-ALS 7 and ALS 9, that are involved in adhesion. N terminus of ALS protein is involved in ligand binding (Loza et al, 2004;Rauceo et al, 2006;Liu & Filler, 2011). Study of ALS-deleted mutants have variable effect on adhesion like expression of C. albicans ALS1 or ALS5 genes in non-adhesive, ALS4 deletion decreases C. albicans adherence to endothelial cell.…”

Section: Adhesion To Vaginal Epitheliamentioning

confidence: 99%

Advanced topical drug delivery system for the management of vaginal candidiasis

et al. 2014

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Vaginal candidiasis or vulvovaginal candidiasis (VC) is a common mucosal infection of vagina, mainly caused by Candida species. The major symptoms of VC are dyspareunia, pruritis, itching, soreness, vagina as well as vulvar erythema and edema. Most common risk factors that lead to the imbalance in the vaginal micro biota are the use of antibiotics, pregnancy, diabetes mellitus, immuno suppression as in AIDS or HIV patients, frequent sexual intercourse, spermicide and intra-uterine devices and vaginal douching. Various anti-fungal drugs are available for effective treatment of VC. Different conventional vaginal formulations (creams, gels, suppositories, powder, ointment, etc.) for VC are available today but have limited efficacy because of lesser residence time on vaginal epithelium due to self-cleansing action of vagina. So to overcome this problem, an extended and intimate contact with vaginal mucosa is desired; which can be accomplished by utilizing mucoadhesive polymers. Mucoadhesive polymers have an excellent binding capacity to mucosal tissues for considerable period of time. This unique property of these polymers significantly enhances retention time of different formulations on mucosal tissues. Currently, various novel formulations such as liposomes, nano-and microparticles, micro-emulsions, bio-adhesive gel and tablets are used to control and treat VC. In this review, we focused on current status of vaginal candidiasis, conventional and nanotechnology inspired formulation approaches.

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Threonine-Rich Repeats Increase Fibronectin Binding in the Candidaalbicans Adhesin Als5p

Cited by 115 publications

References 35 publications

Distinct domains of the Candida albicans adhesin Eap1p mediate cell–cell and cell–substrate interactions

Distinct domains of the Candida albicans adhesin Eap1p mediate cell–cell and cell–substrate interactions

The Role of the Extracellular Matrix (ECM) in Phytopathogenic Fungi: A Potential Target for Disease Control

Advanced topical drug delivery system for the management of vaginal candidiasis

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