Three states of stromal cells–solid, liquid, and aerosol–and innovative delivery methods not previously reported

Abstract

Clinical applications of stromal cells obtained mechanically from adipose tissue are quite popular methods. However, generally accepted protocols still do not exist. In this study, three new delivery methods using different protocols are presented as innovative methods in accordance with an approach called “Indication-based protocols.” In mechanical methods, before cutting the fat tissue with ultra-sharp blades, which we define as “Adinizing,” mixing it with different liquids such as saline or plasma provides the stromal cells in liquid form with high number and viability as a final product. At the same time, since stromal cells and extracellular matrix are preserved by mechanical methods, it was deemed appropriate to use the term total stromal cells (TOST) instead of stromal vascular fraction for this final product, unlike the product obtained with the enzyme. TOST can be combined with plasma and used for dermal filling in “solid” form. In addition to this filling effect, it will also cause a change in the tissue regeneratively. Finally, the stromal cells obtained from liquid can be applied clinically in aerosol form with the help of nebulizer. We believe that three innovative delivery methods can be used successfully in the treatment of many clinical situations in the future.

Using 3D Bioprinted Autologous Minimally Manipulated Homologous Adipose Tissue for Limb Salvage in Treating Diabetic Foot Ulcer

“…However, emerging studies suggest that fat grafts promote wound healing and exert a vasogenic effect. 13 14 15 16 Lipoaspirate, which is obtained during fat grafting, contains cells such as preadipocytes and multipotent adipose-derived stem cells. These cells contribute to a vasogenic effect in hypoxic conditions, thereby facilitating wound healing.…”

“…The mechanism by which fat grafts integrate into the wound site has not been fully elucidated. However, emerging studies suggest that fat grafts promote wound healing and exert a vasogenic effect [13][14][15][16]. Lipoaspirate, which is obtained during fat grafting, contains cells such as preadipocytes and multipotent adipose-derived stem cells (ASCs).…”