Three-dimensional printing of Hela cells for cervical tumor model in vitro

Abstract

Advances in three-dimensional (3D) printing have enabled the direct assembly of cells and extracellular matrix materials to form in vitro cellular models for 3D biology, the study of disease pathogenesis and new drug discovery. In this study, we report a method of 3D printing for Hela cells and gelatin/alginate/fibrinogen hydrogels to construct in vitro cervical tumor models. Cell proliferation, matrix metalloproteinase (MMP) protein expression and chemoresistance were measured in the printed 3D cervical tumor models and compared with conventional 2D planar culture models. Over 90% cell viability was observed using the defined printing process. Comparisons of 3D and 2D results revealed that Hela cells showed a higher proliferation rate in the printed 3D environment and tended to form cellular spheroids, but formed monolayer cell sheets in 2D culture. Hela cells in 3D printed models also showed higher MMP protein expression and higher chemoresistance than those in 2D culture. These new biological characteristics from the printed 3D tumor models in vitro as well as the novel 3D cell printing technology may help the evolution of 3D cancer study.

Current developments in 3D bioprinting for tissue engineering

“…Using this model, they were able to research the morphology, migration and interaction with bone stromal cells of breast cancer cells. Zhao et al [52] used 3D bioprinting to create a new 3D tumour model that showed a higher proliferation rate and a higher chemoresistance compared to the 2D model. This shows that 3D printing can help develop in vitro models that are closer to reality.…”

Bioimpressão e produção de mini-órgãos com células tronco

“…As células HeLa em 3D também formaram esferóides celulares em 3D em contraste com as folhas de células de monocamada formadas em cultura 2D. Os resultados deste estudo usando uma nova técnica de células-impressas em 3D para construção de modelos in vitro de tumor podem ajudar a melhorar a caracterização da formação tumor, sua progressão e a resposta aos tratamentos com medicamentos anticancerígenos (Zhao et al 2014, Knowlton et al 2015.…”

“…Quando modelos de tumores heterogêneos em 3D com vá-rios tipos de células são construídos via bioprinting e livres do arcabouço celular, as células que são co-impressas com células tumorais são utilizadas para produzir MEC naturalmente, evitando o problema das diferenças estruturais entre as proteínas utilizadas e a composição e variando as propriedades dos materiais associados com arcabouços exógenos. Assim, as interações fisiológicas serão independentes entre as células e a matriz, com isso poderá ser compreendida de forma mais direta todo o processo de bioimpressão (Zhao et al 2014).…”

Impression 3D en médecine régénératrice et ingénierie tissulaire

“…Après assemblage, 90 % des cellules étaient vivantes et avaient tendance à former des sphéroïdes alors que les cellules cultivées en 2D restaient en monocouches. Par comparaison aux cellules cultivées en 2D, ces cellules exprimaient plus de métallo-protéases et présentaient une plus grande résistance à la chimiothérapie, les rapprochant de la situation clinique [32]. Les recherches actuelles tentent de modéliser le…”