Three-Dimensional Printing for Craniofacial Bone Tissue Engineering

Shen, Chen; Witek, Lukasz; Flores, Roberto L.; Tovar, Nick; Torroni, Andrea; Coelho, Paulo G.; Kasper, F. Kurtis; Wong, Mark E.; Young, Simon

doi:10.1089/ten.tea.2020.0186

Cited by 32 publications

(39 citation statements)

References 108 publications

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“…in oral and maxillofacial region) is still a huge challenge to traditional hydrogel scaffolds. Although 3D printing is promising in endowing the scaffolds a significant shape-fitness, the requirement of extensive surgical exposure limited its application [ 41 , 42 ]. To overcome this issue, the current study aimed to design an injectable hydrogel that could easily pass through the needle of a syringe and possess the stability to retain in situ .…”

Section: Discussionmentioning

confidence: 99%

Synergistic osteogenic and angiogenic effects of KP and QK peptides incorporated with an injectable and self-healing hydrogel for efficient bone regeneration

Zhou

Luo

et al. 2022

Bioactive Materials

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Section: Discussionmentioning

confidence: 99%

Synergistic osteogenic and angiogenic effects of KP and QK peptides incorporated with an injectable and self-healing hydrogel for efficient bone regeneration

Zhou

Luo

et al. 2022

Bioactive Materials

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“…These studies suggest that sEVs may play an important role in the field of tissue regeneration in the future. Bone tissue engineering technology, which avoids the damages of autologous bone graft, provides a new strategy for repairing of cranio-maxillofacial bone defects (28).…”

Section: Discussionmentioning

confidence: 99%

Osteogenically Induced Human Umbilical Cord Mesenchymal Stem Cell-derived Small Extracellular Vesicles Carrying Distinct microRNA Signature Improve Osteoinductivity of Tissue-engineered Bone

Zhou

Rong

et al. 2022

Preprint

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Background: Clinical application of stem cell-based tissue-engineered bone (TEB) has been severely hindered by a low viability of seed cells, slow osteogenesis and biosafety. Mesenchymal stem cells-derived small extracellular vesicles (sEVs) possess similar properties to donor cells and have low immunogenicity, strong stability and favorable biosafety. It’s validated that human umbilical cord mesenchymal stem cells-derived sEVs (hUCMSCs-sEVs) exert therapeutic effects on various diseases. However, little is known whether hUCMSCs-sEVs can effectively be used to construct TEB. This study aimed to investigate whether hUCMSCs-sEVs bore positive effects on bone regeneration and elucidate the underlying mechanisms. Methods: Herein, PM-sEVs and OM-sEVs were separately harvested from proliferation medium (PM) and osteogenic medium (OM) cultured hUCMSCs and identified. The effects of PM-sEVs and OM-sEVs on the bioactivities and osteogenic differentiation of human bone marrow stromal cells (hBMSCs) were evaluated. The differential microRNAs (miRNAs) expressions between PM-sEVs and OM-sEVs were screened by miRNA sequencing. Furthermore, PM-sEVs and OM-sEVs were separately incorporated in tricalcium phosphate (TCP) scaffolds to build TEBs, which were used to repair critical-sized calvarial bone defects of rats. Results: Both PM-sEVs and OM-sEVs had similar morphology, size and expressed specific surface markers of exosomes while didn’t have the surface marker from donor cells. Both PM-sEVs and OM-sEVs could be internalized by hBMSCs, thus significantly promoting the migration, proliferation and osteogenic differentiation of hBMSCs. Remarkably, OM-sEVs outweighed PM-sEVs in accelerating hBMSCs’ migration and osteogenic differentiation. Besides, miRNA sequencing analysis revealed that several differentially expressed miRNAs might be responsible for sEVs’ bioactivities. Furthermore, OM-sEVs could sustainably release from TCP scaffolds, and the resultant TEB had better reparative outcome than that of PM-sEVs group and pure TCP. Conclusion: sEVs isolated from osteogenically induced hUCMSCs (OM-sEVs) could prominently facilitate osteogenic differentiation of hBMSCs by unique miRNAs signature. TEB graft derived from OM-sEVs provided a promising alternative for clinical transplantation.

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“…They have been used chiefly for interim prostheses and flippers for surgical and occlusal guides. Its clinical applications also extend to working models and main applications in the fields of oral implantology, with the benefits of high material utilization (Guerrero-Girones et al 2022) and the ability to manufacture complex geometries (Shen et al 2020). Nevertheless, 3D printing has expanded to more permanent applications as the technology and materials developed.…”

Section: Three-dimensional Printingmentioning

confidence: 99%

The Forefront of Dentistry—Promising Tech-Innovations and New Treatments

DaSilva

Robinson

Shi³

et al. 2022

JDR Clinical & Translational Research

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Change in our world is happening quickly. For dentistry, this is no exception, and now is the time to foster new opportunities. Advances in the foundational science of dental, oral, and craniofacial health are expanding and enabling personalized care built on strong discoveries in the fields of microbiology, immunology, and neuroscience. Digital communication through electronic health records, patient portals, and teledentistry is emerging as vital new armamentarium for contemporary dental practice. Wearables linked with smartphone apps are trending to monitor pain levels, postoperative symptoms, wound healing, and home oral health habits. Oral fluid diagnostics are rapidly surfacing for use in diagnostics, drug testing, and hormone detection. Manipulation of the oral microbiome is under investigation for caries management and for potential impact on systemic conditions. Resolving mediators of inflammation are promising for addressing the chronic inflammation that plagues periodontal patients. New imaging modalities paired with digital approaches and 3-dimensional printing are formulating disruptions in the technical aspects of dentistry. Augmented reality and virtual reality are providing great potential as teaching tools for dental students and bear promise for lifelong learning for providers. Optimization of information technology and the incorporation of data science partnered with artificial intelligence and machine learning to improve patient care in a learning health care system approach will benefit large numbers of patients. These are exciting times for our profession, yet we need to navigate these paths carefully. Technology needs to significantly and positively change the experience of those involved (patients, dentists, staff, payors). New technologies need to be backed with solid research and implementation science. Automation should focus on improving care with attention to expanding care to the underserved and not lose the critical human connection that our patients need. There is great potential in the emerging innovations yet also great responsibility to ensure they are evidence based on rigorous science and deployable with equity and sensitivity. Knowledge Transfer Statement: This article discusses innovations in technology and treatments that have enormous potential to revolutionize our dental care, including novel concepts in electronic health records, communication between dentists and patients, biologics around diagnosis and treatment, digital dentistry, and, finally, the real-time optimization of information technology. The early implementation and validation of these innovations can drive down their costs and provide better dental and medical services to all members of our society.

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Three-Dimensional Printing for Craniofacial Bone Tissue Engineering

Cited by 32 publications

References 108 publications

Synergistic osteogenic and angiogenic effects of KP and QK peptides incorporated with an injectable and self-healing hydrogel for efficient bone regeneration

Synergistic osteogenic and angiogenic effects of KP and QK peptides incorporated with an injectable and self-healing hydrogel for efficient bone regeneration

Osteogenically Induced Human Umbilical Cord Mesenchymal Stem Cell-derived Small Extracellular Vesicles Carrying Distinct microRNA Signature Improve Osteoinductivity of Tissue-engineered Bone

The Forefront of Dentistry—Promising Tech-Innovations and New Treatments

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