Three Dimensional Imaging of HeLa cells using light sheet based imaging flow cytometry

Review: tomographic imaging flow cytometry

“…However, focusing of the particles in the center of the channel is required to ensure that all the particles are in the focus of the narrow excitation beam. 60 The light-sheet can be formed with a collimator lens and a cylindrical lens in combination with an objective or the lightsheet forming lens that can be directly integrated in the microfluidic device. 61 Also, two lasers can be operated alternately to obtain two fluorescence channels simultaneously.…”

On the robustness of machine learning algorithms toward microfluidic distortions for cell classification via on-chip fluorescence microscopy

“…As a proof of concept, we selected cancer cell classification based on images acquired on a standard 3D light-sheet fluorescence imaging system coupled with microfluidics. 22,23 This microscopy technique has gained remarkable interest in such applications [24][25][26] due to its ability to rapidly scan large 3D samples with reduced phototoxicity. 27 We use this system to study the effect of motion blur and of the variable number of image frames per sample.…”