Three-dimensional culture of dental pulp stem cells in direct contact to tricalcium silicate cements

Widbiller, Matthias; Lindner, Sophia R.; Buchalla, Wolfgang; Eidt, Andreas; Hiller, K-A; Schmalz, Gottfried; Galler, KM

doi:10.1007/s00784-015-1515-3

Cited by 78 publications

(76 citation statements)

References 46 publications

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“…Results from the cytotoxicity assays after 4 days demonstrated significant DPSC viability when in contact with Biodentine as compared with the other groups. Our data are in consensus with Widbiller, et al 32 (2016) who showed that DPSC viability on MTA was significantly lower when compared with that of cells on Biodentine for the first seven days. Nevertheless, other studies have compared the cell viability of human pulp fibroblasts, human gingival fibroblasts, or osteoblast-like cells exposed to Biodentine or MTA and observed similar cell growth without statistically significant differences 2,13,14 .…”

Section: Discussionsupporting

confidence: 93%

Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

et al. 2016

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Objective:The aim of the present study was to evaluate and compare the cytotoxic effects of Biodentine and MTA on dental pulp stem cells (DPSCs) and to assess cell viability and adherence after material exposure to an acidic environment.Material and Methods:DPSCs were cultured either alone or in contact with either: Biodentine; MTA set for 1 hour; or MTA set for 24 hours. After 4 and 7 days, cell viability was measured using the MTT assay. Biodentine and MTA were also prepared and packed into standardized bovine dentin disks and divided into three groups according to the storage media (n=6/group): freshly mixed materials without storage medium (Group A); materials stored in saline (Group B); materials stored in citric acid buffered at pH 5.4 (Group C). After 24 hours, DPSCs were introduced in the wells and cell adherence, viability, and cellular morphology were observed via confocal microscopy after three days of culture. Cell viability was analyzed using repeated-measures analysis of variance test with Tukey's post hoc tests (α=0.05).Results:Biodentine expressed significantly higher cell viability compared with all other groups after 4 days, with no differences after 7 days. Notably, cell viability was significantly greater in 24-hour set MTA compared with 1-hour set MTA and control groups after 7 days. Material exposure to an acidic environment showed an increase in cell adherence and viability in both groups.Conclusions:Biodentine induced a significantly accelerated cell proliferation compared with MTA. Setting of these materials in the presence of citric acid enhanced DPSC viability and adherence.

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Section: Discussionsupporting

confidence: 93%

Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

et al. 2016

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“…The influence of Biodentine ® and MTA on extracellular matrix proteins is versatile. It has been shown in a murine pulp cell line (OD21) [39] and human dental pulp cells [40] that calcium silicate containing materials decrease collagen type I mRNA expression [41, 42]. In our studies we could show that the described reduced collagen mRNA level correlates well with our collagen type I protein concentration analysis.…”

Section: Discussionsupporting

confidence: 85%

Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro

et al. 2016

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Preserving a patient’s own teeth—even in a difficult situation—is nowadays preferable to surgical intervention and therefore promotes development of suitable dental repair materials. Biodentine®, a mineral trioxide aggregate substitute, has been used to replace dentine in a bioactive and biocompatible manner in both the dental crown and the root. The aim of our study was to evaluate the influence of Biodentine® on pulp fibroblasts in vitro. For this study, one to five Biodentine® discs with a diameter of 5.1mm were incubated in DMEM. To obtain Biodentine® suspensions the media were collected and replaced with fresh medium every 24h for 4 days. Primary pulp cells were isolated from freshly extracted wisdom teeth of 20–23 year old patients and incubated with the Biodentine® suspensions. Proliferation, cell morphology, cell integrity and cell viability were monitored. To evaluate the effect of Biodentine® on collagen type I synthesis, the secretion of the N-terminal domain of pro-collagen type I (P1NP) and the release of transforming growth factor-β1 (TGF-β1) were quantified. None of the Biodentine® suspensions tested influenced cell morphology, proliferation or cell integrity. The cell viability varied slightly depending on the suspension used. However, the concentrations of P1NP of all pulp fibroblast cultures treated for 24h with the moderate to high Biodentine® concentration containing suspensions of day 1 were reduced to 5% of the control. Furthermore, a significant TGF-β1 reduction was observed after treatment with these suspensions. It could be shown that Biodentine® is biocompatible. However, dissolved particles of the moderate to high concentrated Biodentine® suspensions 24h after mixing induce a significant reduction of TGF-β1 release and reduce the secretion of collagen type I of primary pulp fibroblasts.

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“…According to Widbiller et al . (), this may be attributed to a potential post‐transcriptional regulation; and a direct comparison is not possible, because of normalization of ALP activity on viable cell culture. It was also noticed that ALP gene expression was lower in the Biodentine group compared to the control group, which is inconsistent with most other studies (Bortoluzzi et al .…”

Section: Discussionmentioning

confidence: 99%

Cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells: an in vitro study

et al. 2018

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Three-dimensional culture of dental pulp stem cells in direct contact to tricalcium silicate cements

Abstract: The tested new tricalcium silicate cement confirms its suitability as an alternative to MTA in vital pulp therapy.

Cited by 78 publications

References 46 publications

Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro

Cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells: an in vitro study

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