Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

Abstract

Objective:The aim of the present study was to evaluate and compare the cytotoxic effects of Biodentine and MTA on dental pulp stem cells (DPSCs) and to assess cell viability and adherence after material exposure to an acidic environment.Material and Methods:DPSCs were cultured either alone or in contact with either: Biodentine; MTA set for 1 hour; or MTA set for 24 hours. After 4 and 7 days, cell viability was measured using the MTT assay. Biodentine and MTA were also prepared and packed into standardized bovine dentin disks and divided into three groups according to the storage media (n=6/group): freshly mixed materials without storage medium (Group A); materials stored in saline (Group B); materials stored in citric acid buffered at pH 5.4 (Group C). After 24 hours, DPSCs were introduced in the wells and cell adherence, viability, and cellular morphology were observed via confocal microscopy after three days of culture. Cell viability was analyzed using repeated-measures analysis of variance test with Tukey's post hoc tests (α=0.05).Results:Biodentine expressed significantly higher cell viability compared with all other groups after 4 days, with no differences after 7 days. Notably, cell viability was significantly greater in 24-hour set MTA compared with 1-hour set MTA and control groups after 7 days. Material exposure to an acidic environment showed an increase in cell adherence and viability in both groups.Conclusions:Biodentine induced a significantly accelerated cell proliferation compared with MTA. Setting of these materials in the presence of citric acid enhanced DPSC viability and adherence.

Concentrated Growth Factor Promotes Dental Pulp Cells Proliferation and Mineralization and Facilitates Recovery of Dental Pulp Tissue

“…MTA can form a hard tissue barrier at the exposed site of pulp, and effectively prevent bacterial leakage [17]. Despite its safety and efficacy, MTA has certain drawbacks, such as poor operability, tooth staining, long setting time, and high cost [39], possibly leading to pulp diffuse calcific deposition and finally resulting in pulp chamber and canal obliteration [1]. In this study, CGF provided evidences that the application of CGF for pulp capping is more similar to the physiological state than are Ca(OH) 2 and MTA.…”

Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA

“…In an in vitro DPC model, BD showed the potential to preserve hDPSC proliferation, migration, and adhesion [17]. Moreover, previous studies comparing the effects of MTA and BD reported that BD accelerated hDPSC proliferation in comparison to MTA [18,19]. TC has been shown to result in superior cell viability of hDPSCs compared to calcium hydroxide [20].…”

Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting

“…13,14 MTA Fillapex is a new calcium carbonate-based sealer with an excellent affinity for MTA-based materials due to MTA's superb biocompatibility, bioactivity, and osteoconductivity. 15 In 2001, MTA Angelus was introduced as a modified form of ProRoot MTA and was used in specific areas. One of the significant differences between ProRoot MTA and MTA Angelus is the absence of dehydrated calcium sulfate, one of its most important ingredients, so that MTA Angelus has a shorter setting time compared to ProRoot MTA.…”