Thiopental improves renal ischemia–reperfusion injury

Doğan, Zafer; Yüzbaşıoğlu, Mehmet Fatih; Kurutaş, Ergül Belge; Yıldız, Hüseyin Bekir; Çoşkuner, İsmail; Şenoğlu, Nimet; Öksüz, Hafize; Bülbüloğlu, Ertan

doi:10.3109/08860221003611752

Cited by 23 publications

(25 citation statements)

References 30 publications

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“…Contrary to this Doğan et al [17] reported that thiopental has beter antioxidant effects than propofol according to their comparative study.…”

Section: Discussionmentioning

confidence: 79%

“…Prophylactic antiapoptotic treatment can be an effective therapeutic strategy for the prevention of I/R injury [22] . Thiopental is a highly lipid-soluble anesthetic, which has indicated antioxidant effect by inhibiting lipid peroxidation in ischemia/reperfusion injury [17] . Protective effects of thiopental for renal ischemia/reperfusion injury was searched in several and compared with the other intravenous agents [13,17,23] .…”

Section: Discussionmentioning

confidence: 99%

“…Thiopental is a highly lipid-soluble anesthetic, which has indicated antioxidant effect by inhibiting lipid peroxidation in ischemia/reperfusion injury [17] . Protective effects of thiopental for renal ischemia/reperfusion injury was searched in several and compared with the other intravenous agents [13,17,23] . Yüzer et al [13] reported that propofol and thiopental anesthesia protects against biochemical, and morphological damage better than control in renal I/R injury.…”

Section: Discussionmentioning

confidence: 99%

“…The protective effects of various intravenous anesthetics, such as ketamine, propofol, etomidate, and thiopental [14][15][16] , against renal I/R damage have been studied [12,13] . Thiopental is known to reduce the release of free oxygen radicals from neutrophils in renal I/R damage, inhibit lipid peroxidation, and demonstrate antioxidant effects by reducing hemolysis of red blood cells caused by free oxygen radicals [17] . However, there are no studies in the literature that posit at what doses thiopental is effective against renal I/R damage.…”

Section: Introductionmentioning

confidence: 99%

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Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

Çoşkuner¹,

Türkay²,

Yıldız³

et al. 2016

Kafkas Univ Vet Fak Derg

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The aim of this study was to determine optimal protective thiopental dose in renal ischemia/reperfusion (I/R) damage. This study was carried out on 42 rats. After abdominal midline incision, left renal artery was occluded. After 60 min of ischemia and reperfusion, a left nephrectomy was performed. Surgical intervention was made for the six rat groups (excluding sham group). In control group, 60 minutes of ischemia and reperfusion were applied to left kidneys. Thiopental (3, 10, 30, 60, 80 mg/ kg) was applied intra-peritoneally to the rats except sham and control groups 15 minutes before reperfusion. After reperfusion, IL-1α, IL-6, TNF-α levels; SOD activity; MDA and NO levels were measured. There was no decrease in IL-6 or TNF-α levels in groups at all doses. IL-1α levels only decreased in the group receiving 3 mg/kg thiopental. Only the group receiving 80 mg/kg observed decrease in MDA levels. The rats receiving 30 mg/kg showed significant increase in SOD levels. The rats receiving 3 mg/ kg detected significant increase in glutathione peroxidase (GPx) levels. The groups receiving 10, 30, and 60 mg/kg thiopental showed decrease in nitric oxide (NO) levels. According to study results, different doses of thiopental affected each biochemical indicator in renal I/R damage. Keywords: Renal Ischemia/Reperfusion, Thiopental, Rat Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi ÖzetÇalışmada ratlarda farklı dozlarda uygulanan tiyopentalin renal I/R hasarına karşı koruyucu optimal dozunun saptanması amaçlandı. Bu çalışma 42 ratta yapıldı. Abdominal orta hat insizyonu sonrası sol renal arter okluzyonu yapıldı. Altmış dakika işemi ve reperfüzyondan sonra sol nefrektomi yapıldı. Altışar rattan oluşan gruplarda (sham grup dışında) cerrahi prosedür uygulandı. Kontrol grubunda sol böbreklere 60 dk işemi ve reperfüzyon uygulandı. Sham ve kontrol grubu dışındaki ratlara reperfüzyondan 15 dk önce tiyopental (3, 10, 30, 60, 80 mg/kg) intra-peritoneal olarak uygulandı. Reperfüzyon sonunda IL-1α, IL-6, TNF-α seviyeleri; SOD aktivitesi; MDA ve NO düzeyleri ölçüldü. Gruplarda IL-6 ve TNF-α seviyelerinde azalma olmadı. Yalnızca 3 mg/kg tiyopental uygulanan grupta IL-1α seviyesi azaldı. MDA düzeylerinde düşme yalnızca 80 mg/kg tiyopental uygulanan grupta görüldü. 30 mg/ kg tiyopental uygulanan ratlarda SOD düzeylerinde anlamlı yükseklik saptandı. 3 mg/kg tiyopental uygulanan ratlarda glutatyon peroksidaz (GPx) düzeylerinde anlamlı yükseklik saptandı. Nitrik oksit seviyesindeki düşme 10, 30 ve 60 mg/kg tiyopental uygulanan gruplarda görüldü. Çalışma sonuçlarına göre, renal işemi reperfüzyon hasarında her bir biyokimyasal belirteci tiyopentalin farklı dozları etkiledi.

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“…Contrary to this Doğan et al [17] reported that thiopental has beter antioxidant effects than propofol according to their comparative study.…”

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

Çoşkuner¹,

Türkay²,

Yıldız³

et al. 2016

Kafkas Univ Vet Fak Derg

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“…Thus, approaches to lessen I/R injury have been extensively studied, and administration of pharmacologic agents such as anesthetics have been shown to be protective against renal I/R injury 2,3 . Dexmedetomidine, a commonly used anesthetic, could exert renoprotective effects on normoglycemic animals when administered before renal I/R [4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury

et al. 2014

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. Design and supervised all phases of the study, critical revision. ABSTRACT PURPOSE:To investigate the effects of acute hyperglycemia on dexmedetomidine-induced preconditioning against renal ischemiareperfusion injury. METHODS:Sprague-Dawley rats were randomly arranged to the normoglycemic (NG) or hyperglycemic group (HG), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and dex (given by dexmedetomidine) groups. Acute hyperglycemia was induced by intraperitoneal injection (i.p.) of 25% glucose (3 g/kg) 45 min before ischemia. Dexmedetomidine (50 μg/kg, i.p.) was administrated 30 min before induction of ischemia. Renal function, histology, apoptosis, expression of Bax, Bcl-2 and phosphorylated AKT (p-AKT) were detected. RESULTS:I/R insult significantly increased the serum levels of blood urea nitrogen and creatinine, apoptotic tubular epithelial cells, expression of Bax and p-AKT, but decreased Bcl-2 expression. All these changes were further enhanced by hyperglycemia (p<0.05). In hyperglycemic condition, there was no statistically difference between the I/R group and Dex group in all the aforementioned detection indexes (p>0.05). CONCLUSION:Acute hyperglycemia attenuates dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury in non-diabetic rats.

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Preparation and Monitoring of Small Animals in Renal MRI

Kaucsár

Hosszú

Seeliger

et al. 2021

Methods in Molecular Biology

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Renal diseases remain devastating illnesses with unacceptably high rates of mortality and morbidity worldwide. Animal models are essential tools to better understand the pathomechanism of kidney-related illnesses and to develop new, successful therapeutic strategies. Magnetic resonance imaging (MRI) has been actively explored in the last decades for assessing renal function, perfusion, tissue oxygenation as well as the degree of fibrosis and inflammation. This chapter aims to provide an overview of the preparation and monitoring of small animals before, during, and after surgical interventions or MR imaging. Standardization of experimental settings such as body temperature or hydration of animals and minimizing pain and distress are essential for diminishing nonexperimental variables as well as for conducting ethical research.This publication is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers.

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Thiopental improves renal ischemia–reperfusion injury

Abstract: Thiopental and propofol, especially thiopental, are more effective to protect renal I/R injury.

Cited by 23 publications

References 30 publications

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury

Preparation and Monitoring of Small Animals in Renal MRI

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