Thin-Layer Chromatography of Some C21, C19 and C18 Steroids

Sallam, L. A. R.; El-Refai, A. H.; El-Kady, I. A.

doi:10.2323/jgam.15.307

Cited by 15 publications

(5 citation statements)

References 1 publication

(1 reference statement)

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“…The chloroform extracts were then washed with distilled water, dried over anhydrous sodium sulphate, filtered and distilled. Using thin layer chromatographic techniques, transformation products in the chloroform extracts were determined as described previously by Sallam et al (1969). Standards of 1 1-a-, 17-aand 21-monohydroxyprogesterone were used to aid comparison of the chloroform-extract contents.…”

Section: Progesterone Transformatlonmentioning

confidence: 99%

Confirmation of the relationships of Aspergillus egyptiacus and Emericella nidulans using progesterone transformation

Ismail

Zohri

1994

Lett Appl Microbiol

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Isolates of Emericella nidulans (7 isolates), Aspergillus egyptiacus (7) and A. versicolor (5) were tested for their ability to transform progesterone. Consistent results were obtained between isolates of the same species. Whilst A. egyptiacus and E. nidulans possessed the enzyme system catalysing the transformation of progesterone into 11‐α‐hydroxyprogesterone, A. versicolor did not. Thus, progesterone transformation could be considered as further diagnostic evidence that A. egyptiacus is related to the Emericella group.

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Section: Progesterone Transformatlonmentioning

confidence: 99%

Confirmation of the relationships of Aspergillus egyptiacus and Emericella nidulans using progesterone transformation

Ismail

Zohri

1994

Lett Appl Microbiol

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“…Steroids were visualized by UV florescence and acid charring (10% H 2 SO 4 , 110°C, 5 min) (Sallam et al 1969).…”

Section: Thin Layer Chromatographymentioning

confidence: 99%

Microbial transformation of diosgenin and its precursor furostanol glycosides

Adham

Zaki

Naim

2008

World J Microbiol Biotechnol

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Microorganisms have been screened for biotransformational activity against diosgenin or its precursor furostanol glycosides obtained from fenugreek seed (Trigonella foenum-graecum). Using diosgenin as the substrate, Cunninghamella elegans and Aspergillus nidulans produced some androstenes when a,a 0 -dipyridyl was supplemented to the transformation medium. Also, using the glycosides as a substrate, Rhizopus sp. produced diosgenin in [90% yield from the glycoside. The parameters were optimized to increase the yield of the androstenes.

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“…The chloroform extracts were then washed with distilled water, dried over anhydrous sodium sulphate, filtered and then distilled. Using thin layer chromatographic techniques, transformation products in the chloroform extracts were determined as described previously by Sallam et al (1969). Standards of steroid compounds were used to aid comparison of the chloroform-extract contents.…”

Section: Progesterone Transformationmentioning

confidence: 99%

Progesterone transformation as a diagnostic feature in the classification of the Aspergillus niger group

Mostafa

1995

Lett Appl Microbiol

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Fifty-five isolates of nine species and two species varieties (5 isolates each) of the Aspergillus niger group were tested for progesterone transformation. Generally, all isolates of the different species transformed progesterone to 21-hydroxyprogesterone. In addition, the isolates of seven species have the capacity to hydroxylate progesterone to 11 alpha-hydroxyprogesterone. A systematic variation could be observed between the tested species of the Aspergillus niger group with respect to the transformation reactions of progesterone. Comparative biotransformation results showed that essential differences exist between the species of this group, where the different isolates of individual species always produce one or more identical products. This biochemical differentiation may supplement the morphological criteria used in the classification of this group of fungi.

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Thin-Layer Chromatography of Some C21, C19 and C18 Steroids

Cited by 15 publications

References 1 publication

Confirmation of the relationships of Aspergillus egyptiacus and Emericella nidulans using progesterone transformation

Confirmation of the relationships of Aspergillus egyptiacus and Emericella nidulans using progesterone transformation

Microbial transformation of diosgenin and its precursor furostanol glycosides

Progesterone transformation as a diagnostic feature in the classification of the Aspergillus niger group

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