Microbial transformation of diosgenin and its precursor furostanol glycosides

Adham, Nehad Z.; Zaki, Rania A.; Naim, Nadia

doi:10.1007/s11274-008-9913-1

Cited by 23 publications

(13 citation statements)

References 19 publications

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“…At low concentration of agar-agar, very fragile gel beads are formed that also results in less enzyme entrapment within their micro-environment and unfortunately, due to the large pore size; the likelihood of enzyme leakage also increases during repeated washing of the beads. Molecular structure of an enzyme also affects the immobilization yield and it was reported that 4.0% agar-agar concentration was optimum for achieving maximum immobilization yield for amylase [13] and lipase [16]. However, for the entrapment of pectinase, 80.0% yield was achieved with 3.0% concentration of agar-agar [12].…”

Section: Agar-agar Concentrationmentioning

confidence: 99%

Agar–agar entrapment increases the stability of endo-β-1,4-xylanase for repeated biodegradation of xylan

Bibi

Shahid

Qader

et al. 2015

International Journal of Biological Macromolecules

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Section: Agar-agar Concentrationmentioning

confidence: 99%

Agar–agar entrapment increases the stability of endo-β-1,4-xylanase for repeated biodegradation of xylan

Bibi

Shahid

Qader

et al. 2015

International Journal of Biological Macromolecules

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“…Curvularia lunata, Penicillium melinii, Aspergillus niger and Rhizopus sp. have been identified to selectively cleave the glycosyl residue of steroidal saponins at C-3 (Feng et al 2005;He et al 2006a;He et al 2006b;Adham et al 2009). More recently, an eco-friendly process with multi-enzymes (including commercial cellulase, amylase, glucoamylase, b-glucosidase, and pectinase) for hydrolysis is used to produce diosgenin from the raw material .…”

Section: Introductionmentioning

confidence: 99%

Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase

Liu

Huang

Sun

et al. 2009

World J Microbiol Biotechnol

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A commercial cellulase was first assessed to be effective in hydrolyzing glycosyl at the C-3 and C-26 positions in steroidal saponins from yellow ginger (Dioscorea zingiberensis C. H. Wright) to diosgenin, a very important chemical in the pharmaceutical industry. The effect of different parameters on enzyme hydrolysis was further investigated by systematically varying them. The highest yield was achieved when the hydrolysis ran at 55°C and pH 5.0 with an enzyme to substrate ratio of 15 × 10(3) U/g. The biotransformed products identified using TLC and HPLC confirmed that the cellulase was capable of releasing diosgenin from steroidal saponins. Moreover, the biotransformation process was explored by LC-MS and LC-MS/MS analysis. Enzymatic hydrolysis together with 40 % of the original sulphuric acid used increased the diosgenin yield by 15.4 ± 2.7% than traditional method. Therefore, the commercial cellulase may serve as a promising tool for industrial diosgenin production and for further use in saponin modification.

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“…The utilization of carbon sources by D. zingiberensis directly affects the growth of strains, which is considered as a prerequisite for biotransformation. Enzymes secreted as part of the metabolism of probiotic Lactobacillus strains could effectively convert saponin to diosgenin and are hence considered as the tool for biotransformation [20]. Lactic acid secreted during fermentation will restrict the growth and biotransformation.…”

Section: Introductionmentioning

confidence: 99%

Screening and identification of probiotic Lactobacillus for the production of diosgenin from Dioscorea zingiberensis Wright by biotransformation

Shu

Wang

Chen

2017

Biotechnology & Biotechnological Equipment

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The commonly used methods to produce diosgenin by acid hydrolysis generate large amounts of wastewater and other pollution. Exploring a new environmentally friendly method is becoming urgent. Mild conditions and less pollution are the advantages of biotransformation. Therefore, aiming at exploring the effect of probiotic Lactobacillus on the production of diosgenin via biotransformation, batch studies were performed. Twenty-four strains maintained in laboratory were screened by conversion rate and pH. Based on screening, purification and 16 s rDNA sequences, four probiotic strains were identified and classified. The results indicated that all the strains had biotransformation potential. Among all the strains, four strains, including L20, L35, L83 and L33, performed better, with conversion rates reaching up to 15.12%, 15.83%, 17.07% and 17.47%, respectively. The highest conversion rate, 17.47%, was that of L33 and the lowest one, 7.07%, of L4. Nineteen of the 24 strains achieved more than 10% conversion rate. The pH decreased from 7 to 3-4 after fermentation, reflecting the progress of fermentation and indicating the end of the biotransformation process. Molecular characterization based on 16S rDNA homology of partial sequences confirmed the preliminary identification as L. rhamnosus (L20, L35), L. paracasei (L83) and L. salivarius (L33).

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Microbial transformation of diosgenin and its precursor furostanol glycosides

Cited by 23 publications

References 19 publications

Agar–agar entrapment increases the stability of endo-β-1,4-xylanase for repeated biodegradation of xylan

Agar–agar entrapment increases the stability of endo-β-1,4-xylanase for repeated biodegradation of xylan

Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase

Screening and identification of probiotic Lactobacillus for the production of diosgenin from Dioscorea zingiberensis Wright by biotransformation

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