Progesterone transformation as a diagnostic feature in the classification of the Aspergillus niger group

Mostafa, Mohamed F.

doi:10.1111/j.1472-765x.1995.tb00438.x

“…In the past, the identification, classification and taxonomy of section Nigri had mainly been based on morphological characteristics. Although some of the species can be readily distinguished morphologically, results obtained in several attempts at classifying this section are debatable and identification of some species is still difficult [3]. Recently, several methods were used in the studies of this section.…”

Section: Introductionmentioning

confidence: 99%

Identification, classification and phylogeny of theAspergillussectionNigriinferred from mitochondrial cytochromebgene

Yokoyama

¹

,

Wang

²

,

Miyaji

³

et al. 2001

FEMS Microbiology Letters

View full text Add to dashboard Cite

The partial mitochondrial cytochrome b gene from 32 strains of 12 species belonging to Aspergillus section Nigri was amplified by the polymerase chain reaction and sequenced directly. Using 402 nucleotide characters, nucleotide-based and amino acid-based phylogenetic trees were inferred and the genetic divergence among the species was evaluated. Based on analyses of the 402-bp nucleotide and 133-amino acid sequences, strains were divided into 11 DNA types and five amino acid types. Aspergillus niger and Aspergillus awamori showed different amino acid sequences. A. niger clade included A. niger var. niger and Aspergillus ficuum. A. awamori clade included A. awamori, Aspergillus phoenicis, Aspergillus pulverulentus, Aspergillus tubingensis, Aspergillus foetidus, and two varieties of A. niger, var. nanus and var. intermedius. Two varieties of A. niger will be reclassified. One strain of A. phoenicis and one strain of Aspergillus carbonarius were reidentified.

show abstract

“…In the past, the identi¢cation, classi¢cation and taxonomy of section Nigri had mainly been based on morphological characteristics. Although some of the species can be readily distinguished morphologically, results obtained in several attempts at classifying this section are debatable and identi¢cation of some species is still di¤cult [3]. Recently, several methods were used in the studies of this section.…”

Section: Introductionmentioning

confidence: 99%

Identification, classification and phylogeny of the Aspergillus section Nigri inferred from mitochondrial cytochrome b gene

Yokoyama

¹

2001

FEMS Microbiology Letters

View full text Add to dashboard Cite

The partial mitochondrial cytochrome b gene from 32 strains of 12 species belonging to Aspergillus section Nigri was amplified by the polymerase chain reaction and sequenced directly. Using 402 nucleotide characters, nucleotide-based and amino acid-based phylogenetic trees were inferred and the genetic divergence among the species was evaluated. Based on analyses of the 402-bp nucleotide and 133-amino acid sequences, strains were divided into 11 DNA types and five amino acid types. Aspergillus niger and Aspergillus awamori showed different amino acid sequences. A. niger clade included A. niger var. niger and Aspergillus ficuum. A. awamori clade included A. awamori, Aspergillus phoenicis, Aspergillus pulverulentus, Aspergillus tubingensis, Aspergillus foetidus, and two varieties of A. niger, var. nanus and var. intermedius. Two varieties of A. niger will be reclassified. One strain of A. phoenicis and one strain of Aspergillus carbonarius were reidentified. ß

show abstract

Progesterone transformation as a biochemical aid in classification of the genusEmericella

Zohri¹

2000

View full text Add to dashboard Cite

A total of 65 isolates representing 13 Emericella taxa (5 isolates of each of 12 species and 1 variety) had the ability to transform progesterone into 11 alpha-hydroxyprogesterone. A systematic variation could be observed between the different tested Emericella taxa with respect to the transformation products. The isolates were divided according to the transformation types into six groups: (1) progesterone was hydroxylated into 6 beta-hydroxyprogesterone, 11 alpha-hydroxyprogesterone and 6 beta,11 alpha-dihydroxyprogesterone--found in Emericella acristata and E. dentata; (2) E. aurantio-brunna and E. parvathecia hydroxylated progesterone into 11 alpha-hydroxyprogesterone, 17 alpha-hydroxyprogesterone and 11 alpha,17 alpha-dihydroxyprogesterone; (3) E. nidulans and E. quadrilineata formed the same three products as members of group (2) and form epicortisol; (4) E. nidulans var. lata, E. bicolor and E. variecolor transformed progesterone into a mixture of mono-, di- and trihydroxy products; (5) E. striata and E. sublata exhibited an oxidative splitting of the progesterone side chain in position C-17 and hydroxylated progesterone into mono- and dihydroxy products; (6) E. rugulosa and E. unguis had the ability to degrade progesterone side-chain and to hydroxylate it into mono-, di- and trihydroxy products. This biochemical differentiation may supplement the morphological and other biochemical criteria used in the classification of the Emericella taxa.

show abstract

Progesterone transformation as a diagnostic feature in the classification of the Aspergillus niger group

Cited by 7 publications

References 19 publications

Identification, classification and phylogeny of theAspergillussectionNigriinferred from mitochondrial cytochromebgene

Identification, classification and phylogeny of theAspergillussectionNigriinferred from mitochondrial cytochromebgene

Identification, classification and phylogeny of the Aspergillus section Nigri inferred from mitochondrial cytochrome b gene

Progesterone transformation as a biochemical aid in classification of the genusEmericella

Contact Info

Product

Resources

About