Thin Layer Chromatography of Pesticides-A Review of Recent Techniques and Applications

Sherma, Joseph

doi:10.1080/01483918208067565

Cited by 25 publications

(10 citation statements)

References 37 publications

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“…The TLC, high‐performance liquid chromatography (HPLC) and gas chromatography (GC) techniques can be used in studies on herbicide metabolism in plants (Mendes et al ., ). In the present study, the TLC technique was used, which presents some advantages such as easy sample preparation and lower cost (Sherma, ). The HPLC technique is one of the most used techniques and recommended for studies with non‐volatile compounds, whereas for volatiles, GC is recommended (Dias et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Absorption, translocation and metabolism of aminocyclopyrachlor in young plants of Ipomoea purpurea and Ipomoea triloba

Silva

Mendes

et al. 2018

Weed Research

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Members of the Convolvulaceae family are known to be sensitive to aminocyclopyrachlor, although little is known about the absorption, translocation and metabolism of the herbicide in these species of weed. The aim of this study was to evaluate the absorption, translocation and metabolism of 14 C-aminocyclopyrachlor in young plants of Ipomoea purpurea and Ipomoea triloba. Assessments were performed at 3, 6, 12, 24, 48 and 72 h after treatment (HAT) for the study of absorption and translocation. Metabolism was assessed at three time points (3, 24 and 72 HAT). In terms of absorption, was observed a difference between species at the 3 and 48 HAT time points, where I. purpurea had a higher absorption of 14 C-aminocyclopyrachlor. No differences were observed between species at any other time points. Of the total absorbed herbicide, 90.9% for I. purpurea and 91.8% for I. triloba were detected on the treated leaf. I. purpurea presented higher translocation to the leaf above the treated leaf, while I. triloba showed higher translocation to the lower leaves and roots. No increase in absorption of 14 C-aminocyclopyrachlor was observed above 24 HAT for I. purpurea and above 6 HAT for I. triloba, and translocation was low (<1%) for both species in all plant parts. This suggests that post-emergence application of aminocyclopyrachlor cannot be effective for the control of I. purpurea and I. triloba and alternative approaches are required. Nevertheless, no 14 C-aminocyclopyrachlor metabolites were observed in the studied plants, which indicated sensitivity in I. purpurea and I. triloba to the herbicide.

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Section: Discussionmentioning

confidence: 99%

Absorption, translocation and metabolism of aminocyclopyrachlor in young plants of Ipomoea purpurea and Ipomoea triloba

Silva

Mendes

et al. 2018

Weed Research

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“…The TLC technique complements the GC and HPLC techniques for the separation, detection, identification and quantification of pesticides, since it has unique advantages when compared to the column chromatography, including: simple preparation of samples, simplicity due to the positioning of the TLC plate on a closed vat containing the mobile phase, and its low operation cost (Sharma, 2005).…”

Section: Measurement Equipmentmentioning

confidence: 99%

“…This technique is called spectroscopy and it may be conducted by several methods, such as infrared, ultraviolet, fluorescence, X-rays, visible light, among others (Sharma, 2005). In order to identify more complex molecules on a sample, the use of mass spectrometry is the most indicated technique, because it is based on the determination of atomic masses, providing information on the structure of these molecules.…”

Section: Measurement Equipmentmentioning

confidence: 99%

Methodologies to Study the Behavior of Herbicides on Plants and the Soil Using Radioisotopes

et al. 2017

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-In Brazil, the "Pesticide Act" (Act no. 7,802/89) has introduced new criteria related to the environment, public health and agronomic performance in the analyses of pesticide-related activities. Likewise, radioisotopes are used for environmental behavior and in planta studies, since they provide some advantages in comparison to chemical measures, including greater sensitivity, stepwise description of a particular element in a metabolic system, and pesticide position and detection through X-ray films and/or radio image (in plants) and liquid scintillation (in plants and soil), respectively. This review describes methodologies related to radioisotope utilization in studies on herbicide absorption, translocation and metabolism in plants, as well as in studies on herbicide biodegradation, mineralization, leaching and sorption-desorption on the soil. The step-by-step of the described methodologies is based on the guidelines that were established, mostly by the Organization for Economic Co-operation and Development (OECD) and the Environmental Protection Agency (EPA). On this review, methodological information on soil and plant studies, using radioisotopes, is available to Brazilian researchers. Thus, the objective of this review is to stimulate the conduction of further studies that use the methodologies described herein. Keywords

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“…Sherma [55,56] has published two reviews on applications of thin -layer chromatography (TLC) and high -performance thin -layer chromatography (HPTLC) for the separation and quantifi cation of pesticides in a variety of food and crop samples. Specifi c applications of carbendazim, thiram, thiophenate -Me, captan, and folpet in fruits and vegetables have been also published [57] .…”

Section: Analysis Of Endocrine Disrupting Compounds In Foodmentioning

confidence: 99%

Pesticides: Herbicides and Fungicides

Falcó

Vidal

Canals

2010

Analysis of Endocrine Disrupting Compounds in Food

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Thin Layer Chromatography of Pesticides-A Review of Recent Techniques and Applications

Cited by 25 publications

References 37 publications

Absorption, translocation and metabolism of aminocyclopyrachlor in young plants of Ipomoea purpurea and Ipomoea triloba

Absorption, translocation and metabolism of aminocyclopyrachlor in young plants of Ipomoea purpurea and Ipomoea triloba

Methodologies to Study the Behavior of Herbicides on Plants and the Soil Using Radioisotopes

Pesticides: Herbicides and Fungicides

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