Thin-layer chromatogram development with a moving pipette delivering the mobile phase onto the surface of the adsorbent layer

Hałka-Grysińska, Aneta; Skop, Kamila; Klimek-Turek, Anna; Gorzkowska, Marta; Dzido, Tadeusz H.

doi:10.1016/j.chroma.2018.08.003

Cited by 15 publications

(4 citation statements)

References 24 publications

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“…The new prototype allows delivering the mobile phase at a controlled velocity to the chromatographic plate by the pipette moved slightly above the adsorbent layer. The similar type of device based on the 3D printer’s mechanism has been reported by our group to test separation efficiency of substances on chromatographic plate with adsorbent layer face down [33]. The equipment presented in the paper is somewhat different in construction and is especially adapted to sample preparation with SFPE.…”

Section: Resultsmentioning

confidence: 99%

Solvent Front Position Extraction with Semi-Automatic Device as a Powerful Sample Preparation Procedure Prior to Quantitative Instrumental Analysis

et al. 2019

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The new prototype device is applied to the Solvent Front Position Extraction (SFPE) sample preparation procedure. The mobile phase is deposited onto the chromatographic plate adsorbent layer by the pipette, which is moved, according to programmed movement path, by a 3D printer mechanism. The application of the prototype device to SFPE procedure leads to the increased repeatability of the results and significant reduction of the analysis time in comparison to the classical procedure of chromatogram development. Additionally, the new equipment allows use procedures that are not possible to run using the classic chromatogram development. In this paper, the results of manual and semi-automatic sample preparation with SFPE are compared and the possible application of this prototype device is discussed.

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Section: Resultsmentioning

confidence: 99%

Solvent Front Position Extraction with Semi-Automatic Device as a Powerful Sample Preparation Procedure Prior to Quantitative Instrumental Analysis

et al. 2019

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“…On the other hand, substances with abilities for interactions as a proton acceptor and dipolar interactions have higher retention in comparison with others in systems with acetonitrile. Acetonitrile is characterized by weak contribution to interaction as a proton acceptor and possesses high dipolar properties [48].…”

Section: Comparison Of Substance Retention and Selectivity In Acn Andmentioning

confidence: 99%

Comparison of the Retention and Separation Selectivity of Aromatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Stationary Phases and Eluents

2020

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In this manuscript, the retention of aromatic hydrocarbons with polar groups has been compared for systems with various nonpolar columns of the types from C3 to C18 and different mobile phases composed of methanol, acetonitrile, or tetrahydrofuran as modifiers. The selectivity separation of the solutes in systems with different adsorbents, when one eluent modifier is swapped by another, has been explained, taking into account molecular interactions of the solutes with components of the stationary phase region (i.e., extracted modifier depending on the chain length of the stationary phase).

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“…Additive manufacturing has become an integral part of open-source, low-cost, and self-manufactured operational steps. − However, it has hardly been used for building complete chromatographic systems . Even beyond that, 2LabsToGo systems have not yet been demonstrated.…”

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2LabsToGo─Recipe for Building Your Own Chromatography Equipment Including Biological Assay and Effect Detection

et al. 2022

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A complete recipe for building your own chromatography equipment from readily available materials is introduced. It combines sample separation (chemistry laboratory) with biological effect detection (biology laboratory). This hyphenation of two disciplines is necessary for prioritizing important compounds in complex samples. Among the thousands of compounds therein, it is often not clear which compounds are the important ones. On the same separation surface, additional detection of biological effects enables and guides substance prioritization. The newly developed open-source 2LabsToGo system for chemical and biological analysis is completely solvent-resistant and, due to miniaturization, environmentally friendly regarding the consumption of materials. It produces comparable results but is 10 times more compact (26 cm × 31 cm × 34 cm), 10 times lighter (6.8 kg), and 55 times less expensive (€ 1717) than current sophisticated commercial devices. As a proof of concept of the first 2LabsToGo system, the quality of different water samples was analyzed since clean water is becoming increasingly rare. In water, most of the thousands of substance signals or features can neither be identified nor classified toxicologically. However, methods that exploit this hyphenated strategy provide answers to such essential safety issues. Drinking or tap water did not show bioactive or toxic compounds, which was expected, whereas biogas or landfill water samples did. The hyphenated 2LabsToGo strategy is affordable and extremely useful for all laboratories with limited equipment but pressing challenges. It is ready to be used in various analytical tasks and applications.

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Thin-layer chromatogram development with a moving pipette delivering the mobile phase onto the surface of the adsorbent layer

Cited by 15 publications

References 24 publications

Solvent Front Position Extraction with Semi-Automatic Device as a Powerful Sample Preparation Procedure Prior to Quantitative Instrumental Analysis

Solvent Front Position Extraction with Semi-Automatic Device as a Powerful Sample Preparation Procedure Prior to Quantitative Instrumental Analysis

Comparison of the Retention and Separation Selectivity of Aromatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Stationary Phases and Eluents

2LabsToGo─Recipe for Building Your Own Chromatography Equipment Including Biological Assay and Effect Detection

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