Comparison of the Retention and Separation Selectivity of Aromatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Stationary Phases and Eluents

Abstract

In this manuscript, the retention of aromatic hydrocarbons with polar groups has been compared for systems with various nonpolar columns of the types from C3 to C18 and different mobile phases composed of methanol, acetonitrile, or tetrahydrofuran as modifiers. The selectivity separation of the solutes in systems with different adsorbents, when one eluent modifier is swapped by another, has been explained, taking into account molecular interactions of the solutes with components of the stationary phase region (i.e., extracted modifier depending on the chain length of the stationary phase).

Estimation of Nicotine in Tobacco Extract Using C8 Column in High Performance Liquid Chromatography

“…C18 column is more hydrophobic in comparison to C8 column; hence the retention time is different. 20 The elution time of nicotine under the proposed method is much less than other methods indicating suitability for rapid determination of nicotine. The theoretical plates for proposed method were adequate.…”