Solvent Front Position Extraction with Semi-Automatic Device as a Powerful Sample Preparation Procedure Prior to Quantitative Instrumental Analysis

Abstract

The new prototype device is applied to the Solvent Front Position Extraction (SFPE) sample preparation procedure. The mobile phase is deposited onto the chromatographic plate adsorbent layer by the pipette, which is moved, according to programmed movement path, by a 3D printer mechanism. The application of the prototype device to SFPE procedure leads to the increased repeatability of the results and significant reduction of the analysis time in comparison to the classical procedure of chromatogram development. Additionally, the new equipment allows use procedures that are not possible to run using the classic chromatogram development. In this paper, the results of manual and semi-automatic sample preparation with SFPE are compared and the possible application of this prototype device is discussed.

Solvent front position extraction with semi-automatic device as a powerful sample preparation procedure to quantitatitation of tryptophan in human plasma

“…As it was presented in our previous papers 30 – 32 , 35 the precision and accuracy of the SFPE technique are based on the premise that both sample components and the internal standard after chromatogram developments were evenly distributed in the focused common zone of the final solvent front position. The obtaining of the final solvent front position was preceded by additional chromatogram development which allowed cleaning the sample solution from matrix components showing weaker and stronger retention than the solutes of interest.…”

“…Thin-layer chromatography experiments were conducted using a prototype device equipped with moving pipette, worked out in the department, driven by 3D machine (Infinum 3D, Lublin, Poland). The device enabled to deliver the solution of the mobile phase to the adsorbent layer with controlled velocity 35 . The results were documented using the TLC Visualizer (CAMAG, Muttenz, Switzerland).…”

“…By finding the appropriate chromatographic system during screening tests and overcoming the problem of the radial chromatography at the stage of application of the sample, all substances of interest were successfully determined with an error of less than 5% 33 . Additionally, the procedure has been streamlined by partially automating it by the application a prototype device equipped with moving pipette delivering a solvent to the adsorbent layer 34,35 . In our previous research we have proven that our sample preparation procedure is accurate, repeatable and (when the prototype automation is applied) also very user-friendly 33,35 .…”

“…Additionally, the procedure has been streamlined by partially automating it by the application a prototype device equipped with moving pipette delivering a solvent to the adsorbent layer 34,35 . In our previous research we have proven that our sample preparation procedure is accurate, repeatable and (when the prototype automation is applied) also very user-friendly 33,35 . However, so far we have not tested the procedure on "real samples", taken directly from patients.…”

Comparison of the Retention and Separation Selectivity of Aromatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Stationary Phases and Eluents

“…Based on these circumstances, in our previous papers we proposed an approach in which separation selectivity can be explained by molecular interactions of solutes and the component of the stationary phase when one modifier is replaced by another [39,41]. The application of this approach to the explanation of separation selectivity changes was demonstrated, among others, for aliphatic hydrocarbons with a polar group [42] and aromatic hydrocarbons with different polar groups [43] in systems with C18 stationary phase type.…”