Thimerosal Determination by High-Pressure Liquid Chromatography

Simultaneous determination of thimerosal and aluminum in vaccines and pharmaceuticals with the use of HPLC method

“…On the other hand, literature data clearly indicate that cation RHg + reacts with DT to form chelates with 1:1 ratio [51,54]. TM, as the compound with polarized S-Hg bond, C 2 H 5 -Hg δ+ -δ− S-C 6 H 4 -COO − Na + , undergoes cleavage in solution induced by, e.g., light or temperature [7,10,[36][37][38][39]. Consequently, thiosalicylate anion is labile [8] and can be exchanged by stronger nucleophile, e.g., DT ligand.…”

“…Determination of thimerosal in pharmaceuticals is carried out in two ways: as total Hg [26][27][28][29][30][31][32][33][34] or directly as thimerosal [35][36][37][38][39][40][41][42][43][44]. The total Hg determination methods require mineralization and atomization of sample to form mercury vapor.…”

“…However, there are also a few methods where there is no need to apply volatile mercury generation since intact TM is determined. Examples include high-performance liquid chromatography (HPLC) [7,10,[35][36][37][38][39][40] and a few electrochemical methods [42] as well as ultraviolet-visible (UV-vis) spectrometry [43,44]. HPLC determination of TM in pharmaceuticals can be realized with spectrophotometric [7,8,[36][37][38], electrochemical [39,40], or inductively coupled plasma mass spectrometry detection [45,46].…”

“…HPLC determination of TM in pharmaceuticals can be realized with spectrophotometric [7,8,[36][37][38], electrochemical [39,40], or inductively coupled plasma mass spectrometry detection [45,46]. The respective spectrophotometric detection is typically realized in UV range [7,[36][37][38]40].…”

Determination of Thiomersal, Lidocaine and Phenylepherine in their Ternary Mixture

“…Whereas, LC was determined by RP-HPLC [26][27][28][29] and electrochemical [11,13] methods. TM was determined by several methods such as spectrophotometric [16], HPLC [15,18,[30][31][32], and electrochemical [19,32] methods. On the other hand, PE and LC were determined together by HPTLC [7] method while TM and PE was determined together by a spectrophotometric [33] method.…”