Simultaneous determination of thimerosal and aluminum in vaccines and pharmaceuticals with the use of HPLC method

Abstract

Summary.A simple and convenient chromatographic method of simultaneous separation, identification, and quantitative determination of thimerosal (TM) (preservative) and aluminum (adjuvant) in vaccines and pharmaceuticals by reversed phase highperformance liquid chromatography (RP-HPLC) with visible (VIS) detection was developed and validated. Due to postcolumn derivatization with dithizone, any interference from matrix was excluded. Similarly, a possibility of on-column decomposition of dithizonates was eliminated. Evaluated detection limits were 0.3 μg TM and 3.0 μg Al, which correspond to the smallest, but possible to recognize, visible peak.

Fitting Models to Data: Residual Analysis, a Primer

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A review of HPLC technique covering its pharmaceutical, environmental, forensic, clinical and other applications

“…The presented method can be applied to vaccines, pharmaceuticals, and other products containing TM and Al. 27…”

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