Determination of Thiomersal, Lidocaine and Phenylepherine in their Ternary Mixture

Abstract

This work is concerned with the development and validation of two accurate, precise and specific spectrophotometric and chromatographic methods for analysis of Phenylepherine (PE), Lidocaine (LC) and Thiomersal (TM) in their ternary mixture and in Chromoderm ® solution. The developed spectrophotometric method is the mean centering of ratio spectra (MCR) spectrophotometric method that depends on using the mean centered ratio spectra in two successive steps which eliminates the derivative steps and therefore the signal to noise ratio is enhanced. On the other hand, the developed chromatographic method isocratic RP-HPLC method that depends chromatographic separation on Zorbax C 18 column using a mobile phase consisting phosphate buffer: acetonitrile: triethylamine (40:60:0.1, by volume pH=6 with orthophosphoric acid). The flow rate was 0.6 mL min -1 and the eluent was monitored at 245 nm.Factors affecting the developed methods were studied and optimized. Moreover, they have been validated as per ICH guidelines. The developed methods have been successfully applied for determination of PE, LC and TM in different laboratory mixtures and in solution dosage form. Also, Statistical analysis of the results of the two developed methods with each other using F and student's t tests showed no significant difference.

Formulation and optimisation of novel transfersomes for sustained release of local anaesthetic

“…Development of an HPLC method as an accurate method for the analysis of lidocaine free-base was necessary because the UV spectrophotometer was the most reported way to quantify the free-base, and all other reported HPLC methods were developed for the analysis of lidocaine HCl. [23][24][25][26][27] Several methods suggested the use of ACN and PBS as mobile phase components for lidocaine analysis. [23][24][25][26] To achieve good peak resolution, the use of a mobile phase with a basic pH has been suggested, while others have proposed the use of MeOH instead of CAN.…”

“…[23][24][25][26][27] Several methods suggested the use of ACN and PBS as mobile phase components for lidocaine analysis. [23][24][25][26] To achieve good peak resolution, the use of a mobile phase with a basic pH has been suggested, while others have proposed the use of MeOH instead of CAN. [27] Therefore, method optimisation and validation were required in order to improve lidocaine free-base analysis and investigation in formulations.…”

Development of Advanced Chemometric-Assisted Spectrophotometric Methods for the Determination of Cromolyn Sodium and Its Alkaline Degradation Products

“…The MCR method compared to other spectrophotometric derivative methods has the advantage of eliminating derivative steps, and consequently enhances the signal-to-noise-ratio. It has been effectively applied for resolving binary and ternary mixtures with severely overlapping spectra in complex matrices [ 43 , 44 , 45 , 46 , 47 ].…”

Three Simple and Economic Spectrophotometric Methods for Simultaneous Determination of Chitosan and Ascorbic Acid

“…It eliminates the derivative steps, therefore it enhances the signal to noise ratio. It has been applied for resolving binary and ternary mixtures in complex samples with unknown matrices [35]. The mathematical explanation of the developed method was illustrated by Afkhami and Bahram [36].…”