Thermostability of Fumonisin B ₁ , a Mycotoxin from Fusarium moniliforme , in Corn

Abstract

Fumonisin B1 (FB1) is a mycotoxin from Fusarium moniliforme that is frequently associated with corn. Thermal treatments are used in many processes concerning this cereal and its derivatives. The thermostablity of this toxin in dry contaminated corn, resulting from F. monUiforme culture, was studied in different time-temperature combinations. FB1 was quantified by instrumentalized thin-layer chromatography after a two-step sequential development and postchromatographic derivatization byp-anisaldehyde. The identity of FB1 in extracts, before and after heat treatments, was confirmed by high-pressure liquid chromatography. For each temperature, the natural logarithm of the ratio of resulting FB1 on initial content (In C/Co) is linearly correlated to exposure time. The calculated half-lives (L50), corresponding to the 50%o value, were 10 min, 38 min, 175 min, and 8 h at 150, 125, 100, and 75°C, respectively. There is a linear relationship between calculated Lsos on a logarithmic scale and temperature. Therefore FB1 is not significantly destroyed by the main drying processes of corn or thermal treatments used for its derivatives. Other associated means are required for detoxification.

Effect of Temperature and Screw Speed on Stability of Fumonisin B1 in Extrusion‐Cooked Corn Grits

“…No loss of FB 1 was observed when F. moniliforme culture material was boiled in water for 30 min (Alberts et al 1990). Dupuy et al (1993), however, reported a 50% reduction in FB 1 levels when dry contaminated corn culture material was heated for 10, 30, and 175 min, and 8 hr at 150, 125, 100, and 75°C, respectively. Scott and Lawrence (1994) reported that FB 1 , when heated at 190°C for 60 min, was ≈20% recovered in dry corn meal and ≈42% recovered in moist corn meal.…”

Growth of Fusarium moniliforme and its biosynthesis of fumonisin B1 on maize grain as a function of different water activities

“…Recent reports indicate the presence of fumonisins in several countries, principally in maize-based products (Pittet et al 1992;Sydenham et al 1992b). Heat treatments that have been studied, or used for decontamination or destruction (Dupuy et al 1993), have not proved fully satisfactory.…”

Effect of inaccurate storage on Fusarium mycotoxins in maize

“…In the next 3 plots (NG-6, NG-8, NG-15), the values measured after harvest were the highest. Since the decrease in fumonisin at a higher temperature begins at 100-120 o C (Dupuy et al, 1993), the higher values suggest that a number of infection nodes were sampled during sampling. Within an item mycotoxins are typically unevenly distributed, so we have to take a sample from multiple locations for representative results.…”