Thermosensitive PNIPAM-peptide conjugate – Synthesis and aggregation

Trzebicka, Barbara; Robak, Barbara; Trzcinska, Roza; Szweda, Dawid; Suder, Piotr; Silberring, Jerzy; Dworak, Andrzej

doi:10.1016/j.eurpolymj.2012.11.005

Cited by 31 publications

(16 citation statements)

References 32 publications

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“…[24,25] Due to the excellent properties, PNIPAM is considered as the gold standard of thermoresponsive polymers and has been used for various purposes. [26,32,[124][125][126] The phase behavior of PNIPAM in alcohol /water solvent mixtures was firstly studied by adding alcohols to PNIPAM-water solutions leading to s shift of the T CP . [76] In general, the LCST transition temperature of PNIPAM is decreased by addition of methanol and a further addition of the alcohol promotes an increased phase transition temperature.…”

Section: Poly(n-isopropylacrylamide)mentioning

confidence: 99%

Polymers with upper critical solution temperature behavior in alcohol/water solvent mixtures

Zhang

Hoogenboom

2015

Progress in Polymer Science

181

164

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Section: Poly(n-isopropylacrylamide)mentioning

confidence: 99%

Polymers with upper critical solution temperature behavior in alcohol/water solvent mixtures

Zhang

Hoogenboom

2015

Progress in Polymer Science

181

164

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“…They could, then, be stabilized for use as nanocarriers as we have described before. [45][46][47][48] The synthesis was carried out using atom transfer radical polymerization (ATRP) in a binary DMSO/H 2 O solvent system. The water solution behavior of P(HEMA-co-OEGMA) with various compositions was studied and compared to determine the impact of HEMA on the thermoresponsive behavior of copolymers and to determine the relationship between the composition of copolymers and the T cp .…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive P(HEMA-co-OEGMA) copolymers: synthesis, characteristics and solution behavior

et al. 2019

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“…PNIPAM, a biostable polymer, has been synthesized by numerous polymerizations techniques ranging from atom transfer radial polymerization (ATRP) to surface-initiated, photoiniferter-mediated photopolymerization to reversible addition fragmentation transfer (RAFT) [76,96,97]. However, for the synthesis of a biodegradable PNIPAM, there are only limited reports [98,99].…”

Section: Degradable Pnipammentioning

confidence: 99%

Drug encapsulated aerosolized microspheres as a biodegradable, intelligent glioma therapy

Floyd

Galperin

Ratner

2015

J Biomedical Materials Res

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Departments of Bioengineering and Chemical EngineeringThe grim prognosis for patients diagnosed with malignant gliomas necessitates the development of new therapeutic strategies for localized and sustained drug delivery to combat tumor drug resistance and regrowth. Here we introduced the novel formulation of drug encapsulated aerosolized microspheres as a biodegradable, intelligent glioma therapy (DREAM BIG Therapy) that is applied post-resection. DREAM BIG Therapy consists of three types of microspheres [poly(lactic acid) (PLA), poly(lactic-co-glycolic acid) (PLGA), and poly(ε-caprolactone) (PCL)] containing various encapsulated chemotherapeutics, suspended in a degradable, aqueous poly(Nisopropylacrylamide) (PNIPAM) solution. The thermoresponsive PNIPAM solution is capable of suspending drug encapsulated microspheres at room temperature which can be "sprayed on" the post-surgical site. The physiological temperature of the treatment site (37°C) would cause PNIPAM solution to solidify and form an adherent gel layer with entrapped microspheres, providing intimate contact with remaining tumor cells. Over time, PLGA (rapid degradation), PLA (medium speed degradation), and then PCL (slow degradation) microspheres would break iv down, releasing their drug payloads in a sequential, multi-drug release directly to the tumor site, addressing cancerous regrowth and tumor drug resistance. This dissertation will address the development of DREAM BIG Therapy, from microsphere formulation to pilot in vivo studies.Initial work focused on developing blank and drug encapsulated microspheres using emulsion techniques. Preliminary studies utilized rhodamine B as a model drug for encapsulation in PLGA, PLA, and PCL particles and optimized formulation parameters to achieve a high encapsulation. Then, gefitinib, IgG, and lomustine were encapsulated in PLGA, PLA, and PCL microspheres, respectively, achieving encapsulation efficiencies greater than 80% and drug loadings greater than 0.3%. The in vitro release of gefitinib and IgG were also studied. Gefitinib released from PLGA microspheres over 40 days while the release of IgG from PLA microspheres was slower, over a year long period.The microsphere-PNIPAM system was tested for aerosolized application ex vivo. The aqueous PNIPAM solution suspended the microspheres and was aerosolized before phase transitioning to an adherent gel layer on the tissue, entrapping the microspheres on the tissue surface. Finally, when tested in vivo, the gefitinib encapsulated PLGA microspheres entrapped in PNIPAM slowed the tumor volume growth of a subcutaneous C6 glioma in comparison to blank PLGA microspheres entrapped in PNIPAM. Overall, this research confirms the potential of DREAM BIG therapy for future use with multiple chemotherapeutics and microsphere types to combat gliomas at a localized site.v

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Thermosensitive PNIPAM-peptide conjugate – Synthesis and aggregation

Cited by 31 publications

References 32 publications

Polymers with upper critical solution temperature behavior in alcohol/water solvent mixtures

Polymers with upper critical solution temperature behavior in alcohol/water solvent mixtures

Thermoresponsive P(HEMA-co-OEGMA) copolymers: synthesis, characteristics and solution behavior

Drug encapsulated aerosolized microspheres as a biodegradable, intelligent glioma therapy

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