Thermobacillus xylanilyticus gen. nov., sp. nov., a new aerobic thermophilic xylan-degrading bacterium isolated from farm soil.

Touzel, Jean Pierre; O’Donohue, Michael; Debeire, P.; Samain, E.; Breton, Claire Le

doi:10.1099/00207713-50-1-315

Cited by 95 publications

(52 citation statements)

References 33 publications

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“…On the basis of phylogenetic evidence and supported by the availability of taxonomically salient characteristics, the genus Bacillus has been dissected into novel genera (Shida et al, 1996 ;Heyndrickx et al, 1998 ;Touzel et al, 2000 ;Fortina et al, 2001), new species have been described (Combet-Blanc et al, 1995 ;Pettersson et al, 1996 ;Heyndrickx et al, 1999) and invalid species have been validated (Sunna et al, 1997 ;Manachini et al, 2000). Only recently, Ahmad et al (2000) described a new species of Saccharococcus, Saccharococcus caldoxylosilyticus.…”

Section: Abbreviationsmentioning

confidence: 99%

Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov.

Fortina¹,

Mora²,

Schümann³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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A polyphasic study was performed on five thermophilic strains belonging to the genus Bacillus, isolated from soil of different geographical areas. 16S rRNA gene sequence analysis placed these isolates in RNA group 5, with Saccharococcus caldoxylosilyticus and [Bacillus] thermoglucosidasius being the closest phylogenetic neighbours. The type species of Saccharococcus, Saccharococcus thermophilus, was only moderately related to these two species and the novel isolates. DNA-DNA hybridization studies and comparison of morphological, chemotaxonomic and phenotypic features supported the close relationship between the novel isolates and Saccharococcus caldoxylosilyticus. These data justify the reclassification of Saccharococcus caldoxylosilyticus. Following the transfer of the validly described Bacillus species of group 5 into the genus Geobacillus, the reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus comb. nov. is proposed. This species can be distinguished genomically from Geobacillus thermoglucosidasius, Geobacillus stearothermophilus, Geobacillus thermodenitrificans and Saccharococcus thermophilus by a specific PCR-RFLP assay targeting the 16S rDNA.

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Section: Abbreviationsmentioning

confidence: 99%

Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov.

Fortina¹,

Mora²,

Schümann³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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“…‡ According to Andersson et al (1995). representatives of rRNA group 5 sensu Ash et al (1991) and the new genus Thermobacillus (Touzel et al, 2000) contain iso-C "':! as the predominant component, but at lower levels, up to 37 and 48 %, respectively.…”

Section: Chemotaxonomic Analysesmentioning

confidence: 99%

Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov.

Fortina¹,

Pukall²,

Schümann³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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“…Although anteiso-C 15 : 0 is the dominant cellular fatty acid in all members of the genus Paenibacillus (Shida et al, 1997), the profile of strain GR21 T differs from those of members of the genus Paenibacillus in the amounts of the fatty acids C 14 : 0 and anteiso-C 17 : 0 . More differences were found in the case of the genus Cohnella (Kämpfer et al, 2006) and particularly in the case of the genus Thermobacillus (Touzel et al, 2000). Strain GR21 T displayed a lipid profile consisting of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), an aminophospholipid (PN), two unknown glycolipids (GL1 and GL2) and an unknown phosphoglycolipid (PGL).…”

mentioning

confidence: 99%

“…Data for Cohnella were taken from Teng et al (2003), Kämpfer et al (2006) and Cho et al (2007) and data for Thermobacillus were taken from Touzel et al (2000) and Watanabe et al (2007). +, Positive; 2, negative; V, variable; ND, no data available.…”

mentioning

confidence: 99%

Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane

Rivas

García‐Fraile

Zurdo-Piñeiro

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A bacterial strain designated GR21 T was isolated from apoplastic fluid of Saccharum officinarum (sugar cane). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family 'Paenibacillaceae', with Paenibacillus as the closest related genus. Within this genus, the closest related species is Paenibacillus xylanilyticus, with 93.4 % similarity to the sequence of the type strain. The isolate has Gram-variable, facultatively anaerobic, rod-shaped cells, motile by polar and subpolar flagella. Round, non-ornamented, central or subterminal spores are formed in unswollen sporangia. The strain is catalase-positive and oxidase-negative on nutrient agar medium. Cellulose and aesculin were hydrolysed, whereas xylan, starch and gelatin were not. Growth was supported by many carbohydrates as carbon sources. Strain GR21 T displayed a lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, an unknown aminophospholipid, two unknown glycolipids and an unknown phosphoglycolipid. MK-7 was the predominant menaquinone and anteiso-C 15 : 0 was the major fatty acid. The DNA G+C content was 57.8 mol%. Phylogenetic and phenotypic analyses, including assimilation of carbon sources and exoenzyme production commonly used for classification within the family 'Paenibacillaceae', showed that strain GR21 T belongs to a new genus within this family, for which the name Saccharibacillus sacchari gen. nov., sp. nov. is proposed. The type strain of Saccharibacillus sacchari is GR21 T (5LMG 24085 T 5DSM 19268 T ).The family 'Paenibacillaceae' currently contains eight genera of Gram-positive, -variable or -negative sporulating bacilli. The description of the most recently described genus of the family, Cohnella (Kämpfer et al., 2006), was based on its phylogenetic relationship with respect to the remaining genera of the family as well as on its polar lipid profile. Some species of this family, mainly belonging to the genus Paenibacillus, have been isolated from the plant rhizosphere, but only Paenibacillus polymyxa has been reported as a plant endophyte (Cho et al., 2006). In the present work, we describe the isolation and identification of a novel bacterium isolated from apoplastic sap of sugar cane (Saccharum officinarum) in Granada (Spain).For the isolation of strain GR21 T , stem pieces were washed with sterile tap water and soaked in 5 % chloramine T solution for 2 min. After removal of the rind with a sterile sharp knife, the cylinder of parenchyma tissue was rinsed with 70 % ethanol and flame-sterilized. Apoplastic sap from the internodes was collected by centrifugation according to Dong et al. (1994), and serial dilutions were streaked on tryptone soybean agar (TSA) and incubated at 30 u C for 7-10 days. After flaming, no contamination was observed when several stem pieces were incubated on TSA plates.Strain GR21 T was grown on nutrient agar for 48 h at 22 u C to check for motility by phase-contrast microscopy using the hanging drop method. Gram staining was carried o...

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Thermobacillus xylanilyticus gen. nov., sp. nov., a new aerobic thermophilic xylan-degrading bacterium isolated from farm soil.

Cited by 95 publications

References 33 publications

Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov.

Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov.

Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov.

Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane

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