Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov.

Fortina, María Grazia; Pukall, Rüdiger; Schümann, Peter; Mora, Diego; Parini, Carlo; Manachini, P. L.; Stackebrandt, Erko

doi:10.1099/00207713-51-2-447

Cited by 86 publications

(19 citation statements)

References 32 publications

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“…Temporary oxic conditions in the reactors may have further favored LAB because LAB tolerate oxygen better than the strictly anaerobic clostridia. The occurrence of Acetobacter , Rummeliibacillus and Ureibacillus , which are strict aerobes, additionally indicated that oxygen influenced the community development . Oxygen in the reactors resulted from substrate input and from low pressure probably causing reactor leakages as the lab equipment was not designed for such pressure conditions.…”

Section: Discussionmentioning

confidence: 99%

Carboxylic acid production from ensiled crops in anaerobic solid‐state fermentation ‐ trace elements as pH controlling agents support microbial chain elongation with lactic acid

Sträuber

Bühligen

Kleinsteuber

et al. 2018

Engineering in Life Sciences

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Background: For the production of carboxylic acid platform chemicals like medium‐chain fatty acids (MCFA) by anaerobic fermentation, pH control is required. However, adding buffer solutions is ineffective in leach‐bed reactors. Aim: In order to increase the MCFA production by maize silage fermentation and to engineer the process we investigated the effect of solid alkaline iron and manganese additives on the process performance and microbial community dynamics. Results: Without additives, the pH dropped to 3.9 and lactic acid bacteria were favored. Total product yields of 207 ± 5.4 g organic acids (C2‐C6) and alcohols per kg volatile solids were reached. The addition of trace elements increased the pH value and the product spectrum and yields changed. With a commercial iron additive, the product yields were higher (293 ± 15.2 g/kgvolatile solids) and supposedly clostridia used lactic acid for microbial chain elongation of acetic acid producing n‐butyric acid. With the addition of pure Fe(OH)3 or Mn(OH)2, the total product yields were lower than in the other reactors. However, increased production of MCFA and the occurrence of distinct bacterial taxa (Lachnospiraceae, Ruminococcaceae and Megasphaera) related to this metabolic function were observed.Conclusions: The application of alkaline trace metal additives as pH stabilizing agents can mitigate spatial metabolic heterogeneities when trace metal deficient substrates like specific crops or residues thereof are applied.

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Section: Discussionmentioning

confidence: 99%

Carboxylic acid production from ensiled crops in anaerobic solid‐state fermentation ‐ trace elements as pH controlling agents support microbial chain elongation with lactic acid

Sträuber

Bühligen

Kleinsteuber

et al. 2018

Engineering in Life Sciences

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“…The 16S rRNA gene sequences from the bacteria isolated in this study were aligned and clustered against those of the genus Ureibacillus (Fortina et al 2001), which was available from GenBank.…”

Section: Methodsmentioning

confidence: 99%

Highly stable meso-diaminopimelate dehydrogenase from an Ureibacillus thermosphaericus strain A1 isolated from a Japanese compost: purification, characterization and sequencing

et al. 2011

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We screened various thermophiles for meso-diaminopimelate dehydrogenase (meso-DAPDH, EC 1.4.1.16), which catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate, and found the enzyme in a thermophilic bacterium isolated from compost in Japan. The bacterium grew well aerobically at around 55°C and was identified as Ureibacillus thermosphaericus strain A1. We purified the enzyme about 47-fold to homogeneity from crude cell extract using five successive purification steps. The molecular mass of the purified protein was about 80 kDa, and the molecule consists of a homodimer with the subunit molecular mass of about 40 kDa. The optimum pH and temperature for the catalytic activity of the enzyme are about 10.5 and 65°C, respectively. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP but not NAD can serve as the electron acceptor. The Km values for meso-diaminopimelate and NADP at 50°C and pH 10.5 are 1.6 mM and 0.13 mM, respectively. The nucleotide sequence of this meso-DAPDH gene encodes a 326-amino acid peptide. When the gene was cloned and overexpressed in Escherichia coli Rosetta (DE3), the specific activity in the crude extract of the recombinant cells was about 18.0-fold higher than in the extract from U. thermosphaericus strain A1. This made more rapid and simpler purification of the enzyme possible.

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“…The biofilm was sampled, and the bacteria were identified with PCR-DGGE. The predominant species was found to be Ureibacillus thermosphaericus of the genus Ureibacillus , which grows at temperatures of 37° to 60°C (Fortina et al, 2001). …”

Section: Resultsmentioning

confidence: 99%

“…Within the Firmicutes phylum, bands 2, 4, 5, 7, 8, and 9 belonged to the genus Ureibacillus , related to a species of U. thermosphaericus (similarity 91.2–100%), which was reported to be a thermophilic bacteria (37–60°C, optimum 50–60°C) with heterotrophic growth in aerobic environments and no ability for nitrate reduction (Fortina et al, 2001). This species was inferred to dominate the nitrification in the cathode of the MFC under low DO and high temperature conditions.…”

Section: Resultsmentioning

confidence: 99%

The Denitrification Characteristics and Microbial Community in the Cathode of an MFC with Aerobic Denitrification at High Temperatures

Zhao

et al. 2017

Front. Microbiol.

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Microbial fuel cells (MFCs) have attracted much attention due to their ability to generate electricity while treating wastewater. The performance of a double-chamber MFC with simultaneous nitrification and denitrification (SND) in the cathode for treating synthetic high concentration ammonia wastewater was investigated at different dissolved oxygen (DO) concentrations and high temperatures. The results showed that electrode denitrification and traditional heterotrophic denitrification co-existed in the cathode chamber. Electrode denitrification by aerobic denitrification bacterium (ADB) is beneficial for achieving a higher voltage of the MFC at high DO concentrations (3.0–4.2 mg/L), while traditional heterotrophic denitrification is conducive to higher total nitrogen (TN) removal at low DO (0.5–1.0 mg/L) concentrations. Under high DO conditions, the nitrous oxide production and TN removal efficiency were higher with a 50 Ω external resistance than with a 100 Ω resistance, which demonstrated that electrode denitrification by ADB occurred in the cathode of the MFC. Sufficient electrons were inferred to be provided by the electrode to allow ADB survival at low carbon:nitrogen ratios (≤0.3). Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) results showed that increasing the DO resulted in a change of the predominant species from thermophilic autotrophic nitrifiers and facultative heterotrophic denitrifiers at low DO concentrations to thermophilic ADB at high DO concentrations. The predominant phylum changed from Firmicutes to Proteobacteria, and the predominant class changed from Bacilli to Alpha, Beta, and Gamma Proteobacteria.

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Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov.

Cited by 86 publications

References 32 publications

Carboxylic acid production from ensiled crops in anaerobic solid‐state fermentation ‐ trace elements as pH controlling agents support microbial chain elongation with lactic acid

Carboxylic acid production from ensiled crops in anaerobic solid‐state fermentation ‐ trace elements as pH controlling agents support microbial chain elongation with lactic acid

Highly stable meso-diaminopimelate dehydrogenase from an Ureibacillus thermosphaericus strain A1 isolated from a Japanese compost: purification, characterization and sequencing

The Denitrification Characteristics and Microbial Community in the Cathode of an MFC with Aerobic Denitrification at High Temperatures

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