Abstract:A bacterial strain designated GR21 T was isolated from apoplastic fluid of Saccharum officinarum (sugar cane). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family 'Paenibacillaceae', with Paenibacillus as the closest related genus. Within this genus, the closest related species is Paenibacillus xylanilyticus, with 93.4 % similarity to the sequence of the type strain. The isolate has Gram-variable, facultatively anaerobic, rod-shaped cells, mo… Show more
“…The G+C content of the DNA was 50.5 mol%, which is slightly lower than the value recorded for S. sacchari LMG 24085 T (57.8 mol%; Rivas et al, 2008). DNA-DNA hybridization was carried out to evaluate the genomic DNA relatedness between strain HR1 T and S. sacchari LMG 24085 T by applying the optical renaturation rate method as described by De Ley et al (1970) with the modifications described by Huß et al (1983) and Escara & Hutton (1980).…”
Section: Strain Hr1mentioning
confidence: 67%
“…The predominant isoprenoid quinone was MK-7, which supports the affiliation of strain HR1 T to the genus Saccharibacillus; in addition, a small amount of MK-6 was detected. This profile is slightly different from that of S. sacchari LMG 24085 T , in which small amounts of both MK-6 and an unidentified menaquinone were detected (Rivas et al, 2008). The polar lipids of strain HR1…”
Section: Strain Hr1mentioning
confidence: 81%
“…T (Rivas et al, 2008), was also the major fatty acid component of strain HR1 T , comprising 50.3 % of the total. The peptidoglycan type was mesodiaminopimelic acid, in line with all other members of the family 'Paenibacillaceae', which includes the genus Saccharibacillus.…”
Section: Strain Hr1mentioning
confidence: 99%
“…nov. is proposed. The type strain is HR1 T (5KCTCThe genus Saccharibacillus of the family 'Paenibacillaceae' was first proposed by Rivas et al (2008) to include a Gramvariable, facultatively anaerobic, motile and rod-shaped bacterium that was negative for catalase and oxidase activities and that had anteiso-C 15 : 0 as the main fatty acid and menaquinone-7 as the major respiratory menaquinone. At present, the genus comprises only one species, Saccharibacillus sacchari.…”
A taxonomic study was performed on strain HR1 T , which was isolated from a desert soil sample collected from Xinjiang Province (China). Cells were aerobic, Gram-positive-staining, pinkpigmented, sporulating rods with a single lateral flagellum. The organism can grow at 15-42 6C and pH 5.0-10.0, optimally at 30-37 6C and pH 6.0-8.0. Growth is inhibited by 6 % NaCl. Analysis of almost-complete 16S rRNA gene sequence revealed that the isolate represents a distinct taxon within the genus Saccharibacillus; Saccharibacillus sacchari LMG 24085T was the nearest relative (97.9 % sequence similarity). DNA-DNA hybridization showed 29.6 % genetic relatedness between strain HR1 T and S. sacchari LMG 24085T . The major isoprenoid quinone was MK-7 and the predominant fatty acid was anteiso-C 15 : 0 (50.3 %). The G+C content of the DNA was 50.5 mol%. Therefore, based on phenotypic criteria and the phylogenetic position, strain HR1T belongs to a previously unidentified species of the genus Saccharibacillus, for which the name Saccharibacillus kuerlensis sp. nov. is proposed. The type strain is HR1 T (5KCTCThe genus Saccharibacillus of the family 'Paenibacillaceae' was first proposed by Rivas et al. (2008) to include a Gramvariable, facultatively anaerobic, motile and rod-shaped bacterium that was negative for catalase and oxidase activities and that had anteiso-C 15 : 0 as the main fatty acid and menaquinone-7 as the major respiratory menaquinone. At present, the genus comprises only one species, Saccharibacillus sacchari.In our search for organisms capable of growing in desert ecosystems, a bacterial strain, designated HR1 T , was isolated from a soil sample collected from the southern desert of Kuerle (41 u 469 N 86 u 079 E), a city at the heart of Xinjiang, the most westerly province of China. The combination of phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA hybridization showed that strain HR1T is a representative of a novel species of the genus Saccharibacillus.
Strain HR1T was isolated using the standard dilutionplating technique on Luria-Bertani (LB) agar medium. The isolate was cultivated routinely on LB agar at 30 u C under aerobic conditions and maintained as a glycerol suspension (20 %, w/v) at 270 u C. Biomass for molecular systematic and chemotaxonomic studies was obtained by culturing strain HR1T in liquid LB medium and harvesting the cells by centrifugation.Amplification and sequencing of the 16S rRNA gene was performed as described by Cui et al. (2001). The sequence obtained was compared with available 16S rRNA gene sequences retrieved from GenBank using the BLAST program (http://www.ncbi.nlm.nih.gov/blast/) to determine an approximate phylogenetic affiliation. Phylogenetic analysis was performed using the software packages PHYLIP (Felsenstein, 1993) and MEGA version 3.1 (Kumar et al., 2001) after multiple alignment of the data by CLUSTAL_X (Thompson et al., 1997). Distances (distance options according to Kimura's two-parameter model; Kimura, ...
“…The G+C content of the DNA was 50.5 mol%, which is slightly lower than the value recorded for S. sacchari LMG 24085 T (57.8 mol%; Rivas et al, 2008). DNA-DNA hybridization was carried out to evaluate the genomic DNA relatedness between strain HR1 T and S. sacchari LMG 24085 T by applying the optical renaturation rate method as described by De Ley et al (1970) with the modifications described by Huß et al (1983) and Escara & Hutton (1980).…”
Section: Strain Hr1mentioning
confidence: 67%
“…The predominant isoprenoid quinone was MK-7, which supports the affiliation of strain HR1 T to the genus Saccharibacillus; in addition, a small amount of MK-6 was detected. This profile is slightly different from that of S. sacchari LMG 24085 T , in which small amounts of both MK-6 and an unidentified menaquinone were detected (Rivas et al, 2008). The polar lipids of strain HR1…”
Section: Strain Hr1mentioning
confidence: 81%
“…T (Rivas et al, 2008), was also the major fatty acid component of strain HR1 T , comprising 50.3 % of the total. The peptidoglycan type was mesodiaminopimelic acid, in line with all other members of the family 'Paenibacillaceae', which includes the genus Saccharibacillus.…”
Section: Strain Hr1mentioning
confidence: 99%
“…nov. is proposed. The type strain is HR1 T (5KCTCThe genus Saccharibacillus of the family 'Paenibacillaceae' was first proposed by Rivas et al (2008) to include a Gramvariable, facultatively anaerobic, motile and rod-shaped bacterium that was negative for catalase and oxidase activities and that had anteiso-C 15 : 0 as the main fatty acid and menaquinone-7 as the major respiratory menaquinone. At present, the genus comprises only one species, Saccharibacillus sacchari.…”
A taxonomic study was performed on strain HR1 T , which was isolated from a desert soil sample collected from Xinjiang Province (China). Cells were aerobic, Gram-positive-staining, pinkpigmented, sporulating rods with a single lateral flagellum. The organism can grow at 15-42 6C and pH 5.0-10.0, optimally at 30-37 6C and pH 6.0-8.0. Growth is inhibited by 6 % NaCl. Analysis of almost-complete 16S rRNA gene sequence revealed that the isolate represents a distinct taxon within the genus Saccharibacillus; Saccharibacillus sacchari LMG 24085T was the nearest relative (97.9 % sequence similarity). DNA-DNA hybridization showed 29.6 % genetic relatedness between strain HR1 T and S. sacchari LMG 24085T . The major isoprenoid quinone was MK-7 and the predominant fatty acid was anteiso-C 15 : 0 (50.3 %). The G+C content of the DNA was 50.5 mol%. Therefore, based on phenotypic criteria and the phylogenetic position, strain HR1T belongs to a previously unidentified species of the genus Saccharibacillus, for which the name Saccharibacillus kuerlensis sp. nov. is proposed. The type strain is HR1 T (5KCTCThe genus Saccharibacillus of the family 'Paenibacillaceae' was first proposed by Rivas et al. (2008) to include a Gramvariable, facultatively anaerobic, motile and rod-shaped bacterium that was negative for catalase and oxidase activities and that had anteiso-C 15 : 0 as the main fatty acid and menaquinone-7 as the major respiratory menaquinone. At present, the genus comprises only one species, Saccharibacillus sacchari.In our search for organisms capable of growing in desert ecosystems, a bacterial strain, designated HR1 T , was isolated from a soil sample collected from the southern desert of Kuerle (41 u 469 N 86 u 079 E), a city at the heart of Xinjiang, the most westerly province of China. The combination of phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA hybridization showed that strain HR1T is a representative of a novel species of the genus Saccharibacillus.
Strain HR1T was isolated using the standard dilutionplating technique on Luria-Bertani (LB) agar medium. The isolate was cultivated routinely on LB agar at 30 u C under aerobic conditions and maintained as a glycerol suspension (20 %, w/v) at 270 u C. Biomass for molecular systematic and chemotaxonomic studies was obtained by culturing strain HR1T in liquid LB medium and harvesting the cells by centrifugation.Amplification and sequencing of the 16S rRNA gene was performed as described by Cui et al. (2001). The sequence obtained was compared with available 16S rRNA gene sequences retrieved from GenBank using the BLAST program (http://www.ncbi.nlm.nih.gov/blast/) to determine an approximate phylogenetic affiliation. Phylogenetic analysis was performed using the software packages PHYLIP (Felsenstein, 1993) and MEGA version 3.1 (Kumar et al., 2001) after multiple alignment of the data by CLUSTAL_X (Thompson et al., 1997). Distances (distance options according to Kimura's two-parameter model; Kimura, ...
“…They are negative for oxidase activity, have anteiso-C 15 : 0 as the major fatty acid and menaquinone-7 (MK-7) as the major respiratory menaquinone (Rivas et al, 2008). At the time of writing, the genus Saccharibacillus comprises only two species with validly published names: Saccharibacillus sacchari (Rivas et al, 2008) and Saccharibacillus kuerlensis (Yang et al, 2009).…”
A Gram-stain-positive, facultatively anaerobic bacterial strain, designated WLJ055 T , with polar and subpolar flagella was isolated from the top layer of desert soil from Erdos, Inner Mongolia, northern China. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain WLJ055T was a member of the genus Saccharibacillus, and shared 97. During an analysis of the bacterial diversity in the Kubuqi desert, a bacterial strain, designated WLJ055 T , was isolated from the top layer of soil (0-15 cm) collected from the Kubuqi desert (408 189 N 1098 289 E), Ordors, Inner Mongolia, northern China, by using the 10-fold dilution plating technique on Luria-Bertani (LB) agar (g l 21 : tryptone 10.0, yeast extract 5.0, NaCl 10.0, and agar 20.0; pH 7.0). The isolate was cultivated at 30 8C under aerobic conditions and purified by repeatedly streaking on LB agar. Biomass for molecular systematic and chemotaxonomic analyses was prepared by culturing strain WLJ055 T in liquid LB medium at 30 8C for two days and harvesting the cells by centrifugation.The genomic DNA was extracted by using a DNA extraction kit (BioTech) according to the manufacturer's instructions. The 16S rRNA gene was amplified with the universal primer set, 27F (59-AGA GTT TGA TCM TGG CTC AG-39) and 1492R (59-TAC GG(C/T) TAC CTT GTT ACG ACT T-39) by using the protocol described earlier (Wang et al., 2007). The PCR amplicon was purified with a PCR purification kit (Biotech) and sequenced after the resulting DNA fragments were ligated to a pMD19-T vector (TaKaRa) according to the manufacturer's instructions. The nearly complete (1380 bp) sequence was compared with the available 16S rRNA genes of strains of validly published species using the BLAST program (http:// www.ncbi.nlm.nih.gov/blast) to determine its phylogenetic affiliation. Results of BLAST searches indicated that the sequence shared a relatively high level of similarity (approximate 97.2 %) with sequences of species of the genus Saccharibacillus. In order to elucidate the phylogenetic position of strain WLJ055 T , the 16S rRNA gene sequences for type strains of species of the genus Saccharibacillus as well as of the related genus, Paenibacillus were downloaded. Sequences were aligned using CLUSTAL X software (Thompson et al., 1997). Phylogenetic trees were 3These authors contributed equally to this work.
Sugarcane is an important cash crop for many countries because it is a major source of several products including sugar and bioethanol. To obtain maximum yields there is a need to apply large quantities of chemical fertilizers.Worldwide yields are also severely affected by more than sixty diseases, mostly caused by fungi but viruses, phytoplasmas, nematodes and other pests can also damage this crop. For most of these diseases, chemical control is not available and breeders are struggling with the development of pest resistant varieties. Many members of the grass family Poaceae establish associations with beneficial microbes which promote their growth by direct and indirect mechanisms. They can be used as means to reduce the need for chemical fertilizer and to minimize the impacts of pathogen invasion. This review highlights the diversity of the microbes associated with sugarcane and the role of beneficial microbes for growth promotion and biocontrol. More extensive use of beneficial microbes will help the sugarcane grower not only to reduce the use of chemical fertilizers but also minimize the disease. In this paper, a brief description of both the non-pathogenic and pathogenic microbes associated with sugarcane is provided. Future prospects for the expanded use of beneficial microbes for sugarcane are also discussed and detailed herein.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.