Thermobacillus composti sp. nov., a moderately thermophilic bacterium isolated from a composting reactor

Watanabe, Keiko; Nagao, Norio; Yamamoto, Shuich; Toda, Tatsuki; Kurosawa, Norio

doi:10.1099/ijs.0.64672-0

Cited by 38 publications

(19 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Perturbation of the enriched communities by inoculation into cultures with low biomass (1% switchgrass) and amendment with CMC did not significantly alter the community composition of the enrichments, although the glycoside hydrolase activities measured in the supernatant were higher. This observation indicates that biomass loading is a critical component of enrichment cultiva- (2,31,35). Additionally, compost and swine waste-derived microbial communities enriched on microcrystalline cellulose at elevated temperatures under aerobic conditions also contain thermophilic paenibacilli, suggesting that these microbes are critical components of biomass deconstruction under thermophilic aerobic conditions (22,26).…”

Section: Discussionmentioning

confidence: 92%

Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass

Gladden

Allgaier

Miller

et al. 2011

Appl Environ Microbiol

114

View full text Add to dashboard Cite

Industrial-scale biofuel production requires robust enzymatic cocktails to produce fermentable sugars from lignocellulosic biomass. Thermophilic bacterial consortia are a potential source of cellulases and hemicellulases adapted to harsher reaction conditions than commercial fungal enzymes. Compost-derived microbial consortia were adapted to switchgrass at 60°C to develop thermophilic biomass-degrading consortia for detailed studies. Microbial community analysis using small-subunit rRNA gene amplicon pyrosequencing and short-read metagenomic sequencing demonstrated that thermophilic adaptation to switchgrass resulted in low-diversity bacterial consortia with a high abundance of bacteria related to thermophilic paenibacilli, Rhodothermus marinus, and Thermus thermophilus. At lower abundance, thermophilic Chloroflexi and an uncultivated lineage of the Gemmatimonadetes phylum were observed. Supernatants isolated from these consortia had high levels of xylanase and endoglucanase activities. Compared to commercial enzyme preparations, the endoglucanase enzymes had a higher thermotolerance and were more stable in the presence of 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]), an ionic liquid used for biomass pretreatment. The supernatants were used to saccharify [C2mim][OAc]-pretreated switchgrass at elevated temperatures (up to 80°C), demonstrating that these consortia are an excellent source of enzymes for the development of enzymatic cocktails tailored to more extreme reaction conditions.

show abstract

Section: Discussionmentioning

confidence: 92%

Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass

Gladden

Allgaier

Miller

et al. 2011

Appl Environ Microbiol

114

View full text Add to dashboard Cite

show abstract

“…2, ten clones were closely related to Thermobacillus composti strain KWC4 and Thermobacillus xylanilyticus. Strain KWC4 is a Gram-negative, rod-shaped, spore-forming, and moderately thermophilic bacterium isolated from a composting reactor and grows optimally at 50°C [13]. T. xylanilyticus is an aerobic, thermophilic, and xylanolytic spore-forming bacterium and was isolated from farm soil situated underneath a manure heap in Northern France [14].…”

Section: Resultsmentioning

confidence: 99%

Toward Plant Cell Wall Degradation Under Thermophilic Condition: A Unique Microbial Community Developed Originally from Swine Waste

Liang

Yesuf

Feng

2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

A unique thermophilic microbial community developed initially from swine waste was investigated in this study. Cellulase activities were observed when this community was inoculated to media containing either cellulose or carboxymethylcellulose at 57 degrees C. Through constructing a clone library for the 16S ribosomal DNA, it was revealed that this community was mainly composed of three genera: Thermobacillus, Brevibacillus, and Anoxybacillus. New findings regarding the thermo- and pH stability of crude cellulases secreted by Brevibacillus sp. JXL were presented. Recent study on the growth characteristics of Anoxybacillus sp. 527 was discussed.

show abstract

“…Data for Cohnella were taken from Teng et al (2003), Kämpfer et al (2006) and Cho et al (2007) and data for Thermobacillus were taken from Touzel et al (2000) and Watanabe et al (2007). +, Positive; 2, negative; V, variable; ND, no data available.…”

mentioning

confidence: 99%

Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane

Rivas

García‐Fraile

Zurdo-Piñeiro

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

A bacterial strain designated GR21 T was isolated from apoplastic fluid of Saccharum officinarum (sugar cane). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family 'Paenibacillaceae', with Paenibacillus as the closest related genus. Within this genus, the closest related species is Paenibacillus xylanilyticus, with 93.4 % similarity to the sequence of the type strain. The isolate has Gram-variable, facultatively anaerobic, rod-shaped cells, motile by polar and subpolar flagella. Round, non-ornamented, central or subterminal spores are formed in unswollen sporangia. The strain is catalase-positive and oxidase-negative on nutrient agar medium. Cellulose and aesculin were hydrolysed, whereas xylan, starch and gelatin were not. Growth was supported by many carbohydrates as carbon sources. Strain GR21 T displayed a lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, an unknown aminophospholipid, two unknown glycolipids and an unknown phosphoglycolipid. MK-7 was the predominant menaquinone and anteiso-C 15 : 0 was the major fatty acid. The DNA G+C content was 57.8 mol%. Phylogenetic and phenotypic analyses, including assimilation of carbon sources and exoenzyme production commonly used for classification within the family 'Paenibacillaceae', showed that strain GR21 T belongs to a new genus within this family, for which the name Saccharibacillus sacchari gen. nov., sp. nov. is proposed. The type strain of Saccharibacillus sacchari is GR21 T (5LMG 24085 T 5DSM 19268 T ).The family 'Paenibacillaceae' currently contains eight genera of Gram-positive, -variable or -negative sporulating bacilli. The description of the most recently described genus of the family, Cohnella (Kämpfer et al., 2006), was based on its phylogenetic relationship with respect to the remaining genera of the family as well as on its polar lipid profile. Some species of this family, mainly belonging to the genus Paenibacillus, have been isolated from the plant rhizosphere, but only Paenibacillus polymyxa has been reported as a plant endophyte (Cho et al., 2006). In the present work, we describe the isolation and identification of a novel bacterium isolated from apoplastic sap of sugar cane (Saccharum officinarum) in Granada (Spain).For the isolation of strain GR21 T , stem pieces were washed with sterile tap water and soaked in 5 % chloramine T solution for 2 min. After removal of the rind with a sterile sharp knife, the cylinder of parenchyma tissue was rinsed with 70 % ethanol and flame-sterilized. Apoplastic sap from the internodes was collected by centrifugation according to Dong et al. (1994), and serial dilutions were streaked on tryptone soybean agar (TSA) and incubated at 30 u C for 7-10 days. After flaming, no contamination was observed when several stem pieces were incubated on TSA plates.Strain GR21 T was grown on nutrient agar for 48 h at 22 u C to check for motility by phase-contrast microscopy using the hanging drop method. Gram staining was carried o...

show abstract

Thermobacillus composti sp. nov., a moderately thermophilic bacterium isolated from a composting reactor

Cited by 38 publications

References 16 publications

Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass

Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass

Toward Plant Cell Wall Degradation Under Thermophilic Condition: A Unique Microbial Community Developed Originally from Swine Waste

Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane

Contact Info

Product

Resources

About