Thermal preparation of lysozyme-imprinted microspheres by using ionic liquid as a stabilizer

Abstract

Thermal preparation of lysozyme-imprinted microspheres was firstly investigated by using biocompatible ionic liquid (IL) as a thermal stabilizer. The imprinted microspheres made with IL could obtain the good recognition ability to template protein, whereas the imprinted polymer synthesized in the absence of it had a similar adsorption capacity to the non-imprinted one. Furthermore, the preparation conditions of imprinted polymers (MIPs) including the content of IL, temperature of polymerization, and types of functional monomers and crosslinkers were systematically analyzed via circular dichroism spectrum and activity assay. The results illustrated that using hydroxyethyl acrylate as the functional monomer, ethylene glycol dimethacrylate as the crosslinker, 5 % IL as the stabilizer, and 75 °C as the reaction temperature could retain the structure of template protein as much as possible. The obtained MIPs showed excellent recognition ability to the template protein with the separation factor and selectivity factor value of 4.30 and 2.21, respectively. Consequently, it is an effective way to accurately imprint and separate template protein by cooperatively using circular dichroism spectroscopy and activity assay during the preparation of protein MIPs. The method of utilizing IL to stabilizing protein at high temperature would offer a good opportunity for various technologies to improve the development of macromolecules imprinting.

Novel polydopamine imprinting layers coated magnetic carbon nanotubes for specific separation of lysozyme from egg white

“…protein is summarized briefly in Table 3. As can be seen, the resultant nanomaterials prepared in this work has a higher IF than those of other polymers [19, [35][36][37][38][39][40][41]. From this comparison, it can be found that MCNTs@Lyz-MIPs reported here constitute a significant improvement.…”

“…Lyz-imprinted glass substrates ≈ 3.00 [38] Lyz-imprinted microspheres 2.36 [39] Lyz-imprinted thermo-responsive nanogel 2.47 [40] Lyz-imprinted silica submicroparticles 2.53 [41] MCNTs@Lyz-MIPs 4.00 This work…”

Adsorptive removal of enrofloxacin with magnetic functionalized graphene oxide@ metal–organic frameworks employing D‐optimal mixture design

“…However, the structure of the sorbent, especially the shape and size of the sorbent pores, is highly dependent on the type of synthesis method. Among them, the thermal procedure is a simple and efficient method that requires no sophisticated instrument and can control the sorbent structure by controlling temperature and heating time (Li, Zhao, Ma, & Zhao, 2016; Qian et al., 2014; Zhang et al., 2016).…”

Preparation of lysozyme-imprinted nanoparticles on polydopamine-modified titanium dioxide using ionic liquid as a stabilizer

“…[18][19][20] As a result, chol dhp could help maintain template stability during MIP preparation and resulted in effective imprinting and recognition of proteins. 21,22 Recently, dopamine (DA), being able to self-polymerizes in aqueous conditions at room temperature, has been increasingly used as the monomer to prepare MIPs for imprinting fragile proteins and enzymes in a simple yet highly efficient way. [23][24][25] Further, Li et al 26 found that MIPs formed by DA selfpolymerization on polydopamine (PDA)-modied silica particles were able to prevent viral infections, while MIPs formed by DA self-polymerization on pure silica particles had no such effect.…”