A novel sorbent based on a mixture of magnetic functionalized graphene oxide and MOFs was developed to remove enrofloxacin (EFX) from water samples. The prepared sorbent was characterized using Fourier transform infrared spectra, scanning electron microscope images, and X‐ray powder diffraction pattern. The sorbent compositions were optimized by the mixture experimental design. Under the optimal condition, the percentages of each sorbent component, including triethylene tetramine‐functionalized graphene oxide (FGO), Fe3O4, and MOF‐5, were 40%, 21%, and 39%, respectively. Besides, the intraparticle diffusion and pseudo‐second‐order kinetic models can describe the EFX adsorption procedure because of two adsorption mechanisms of EFX on FGO and MOF‐5. A positive standard enthalpy of 49.80 kJ/mol indicated the EFX adsorption is endothermic with a chemisorption process. The negative values of ΔGo obtained in the range of −8.979 to −3.431 kJ/mol at all studied temperatures showed that the adsorption process was also spontaneous. The Langmuir and Freundlich isotherm models were analyzed with the partition coefficient to reduce bias in the isotherm model evaluation. The maximum adsorption capacity of 344.83 mg/g and a high partition coefficient of 17.42 g/L in an initial EFX concentration of 10 mg/L were obtained for the EFX removal. Practitioner points Magnetic functionalized graphene oxide @MOF‐5 as a sorbent for the enrofloxacin removal is synthesized. The percentage amount of each component of the sorbent is optimized using the D‐optimal mixture design. Adsorption mechanisms of enrofloxacin on magnetic functionalized graphene oxide @MOF‐5 are discussed. Thermodynamic parameters for the enrofloxacin adsorption with the sorbents are determined. Isotherm model for the enrofloxacin removal with the sorbent is investigated.