Therapeutic Trial of the Synthetic Heparin Analogue Dextran Sulphate

The Blood Anticoagulant Effect of Short Chain‐length Dextran Sulphates

“…of the preparation U 20, which has a longer molecular chain. Another useful comparison is the result obtained with the dextran sulphate fraction 1 7.6 which has the biological properties advocated for a clinical anticoagulant (Ricketts and Walton, 1953). The effect with this fraction at 10 mg./kg.…”

“…chlorosulphonic acid in 70 ml. pyridine, and the dextran sulphate was isolated as described in a previous paper (Ricketts, 1952a). The weight of dextran sulphate obtained and its sulphur content are recorded in Table I.…”

“…Anticoagulant activity in vitro was measured using recalcified horse plasma (Ricketts and Walton, 1953). To avoid the uncertainties of comparing various dextran sulphates with heparin a dextran sulphate preparation was adopted as a standard.…”

“…Sulphuric esters of the lower members of the maltosaccharide series showed less anticoagulant effect in rabbits than was expected on the basis of activity measurements in vitro (Ricketts, 1952b). This paper describes the preparation ofsome dextran sulphates of short chain-length, their anticoagulant activity in vitro, and their effect upon the blood clotting tme of the rabbit.…”

“…The relation between the degree of sulphation and anticoagulant activity for molecules of this size has been explored (Ricketts, 1952a). In extending the work to compounds of shorter chain-length, some experiments were made with sulphuric esters of oligosaccharides in the maltose series, because they were the only oligosaccharides characterized at the time.…”