Therapeutic potentials of n-hexane extracts of the three medicinal mushrooms regarding their anti-colon cancer, antioxidant, and hypocholesterolemic capabilities

Abstract

Abstract. Daba GM, Elkhateeb WA, El-Dien AN, Ahmed EF, El Hagrassi AM Fayad W, Wen TC. 2020. Therapeutic potentials of n-hexane extracts of the three medicinal mushrooms regarding their anti-colon cancer, antioxidant, and hypocholesterolemic capabilities. Biodiversitas 21: 2437-2445. Evaluating in vitro biological activities of the medicinal mushrooms Hericium erinaceus, Metacordyceps neogunnii, and Dictyophora indusiata n-hexane extracts revealed their capabilities as promising therapeutic sources. By assessing their DPPH radical scavenging activities, D. indusiata extract exhibited the highest antioxidant activity (87.8±1.2%) followed by H. erinaceus, then M. neogunnii 84.9±1.6%, and 77.3±1.3%, respectively. On the other hand, M. neogunnii extract exerted promising anti-colon cancer (68.6±3.6% cytotoxicity) against HCT116 human colon cancer cell lines at concentration 100 μg/mL, whereas H. erinaceus and D. indusiata extracts exhibited weaker cytotoxic effects (18.3±1.7%, and 19.3±3.2%, respectively) using the same concentration. Investigating cholesterol reduction activities (CRA%) of the extracts revealed that activity depends on both extract concentration and incubation period. After 96 h of incubation at room temperature, all extracts exerted potent in vitro hypocholesterolemic activities (100±0% reduction in cholesterol concentration). Performing GC-Ms analyses on the extracts revealed presence of 22 compounds in D. indusiata extract, while 29 compounds were detected in M. neogunnii extract, and 33 in H. erinaceus extract. Most of compounds were esters of fatty acids. Results in this study encourage using these mushrooms as functional foods, and highlight the need for conducting further in vivo studies to support their use in pharmaceutical industry.

Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract

“…Moreover, being edible encourages further investigation to authorize their application in medicines and pharmaceuticals. Studies on many mushroom species such as Ganoderma lucidum, Cordyceps militaris, Hericium erinaceus, Metacordyceps neogunnii (Synonym of Keithomyces neogunnii), and Dictyophora indusiata revealed their potent in vitro biological capabilities as anticoagulant, antiviral, hypocholesterolemic, anti-colon cancer, and antioxidant agents (Elkhateeb et al 2019b;Elkhateeb et al 2019c;El-Hagrassi et al 2020;Daba et al 2020). Furthermore, some mushrooms have promising wound healing activities (Buko et al 2019;Elkhateeb et al 2019c;Kosanić et al 2020).…”

“…Samples were collected on the 1 st of June 2018 and were immediately sliced and dried in a commercial heated dehydrator at approximately 75°C (GP-102-1, 250W, LASER 2000, Westfalia Werkzeug company GmbH & Co KG, Germany). After dehydration, samples were extracted as described previously (Elkhateeb et al 2019d;Daba et al 2020). Briefly, C. squamosus fruiting body pieces were placed in an Erlenmeyer flask containing 80% methanol at room temperature and kept overnight before filtering.…”

Mycotherapy of the good and the tasty medicinal mushrooms Lentinus, Pleurotus, and Tremella

“…Medicinal mushrooms produce a wide range of bioactive compounds [1]. Medicinal mushrooms possess medicinal properties such as anti-tumor, immunomodulating, antioxidant, cardiovascular, anti-hypercholesterolemic, anti-viral, anti-bacterial, antiparasitic, antifungal, detoxification, hepatoprotective, and anti-diabetic effects [2][3][4][5][6][7][8]. Hence, mushrooms have gained a lot of attention as a functional food and for the development of drugs and nutraceuticals.…”

Highlights on the Golden Mushroom Cantharellus cibarius and unique Shaggy ink cap Mushroom Coprinus comatus and Smoky Bracket Mushroom Bjerkandera adusta Ecology and Biological Activities

“…All together with a long history as food source, mushrooms are important for their healing capacities and properties in traditional medicine [7][8][9][10][11][12][13][14][15]. It has reported beneficial effects for health and treatment of some diseases.…”