Therapeutic potential of human adipose stem cells in a cancer stem cell-like gastric cancer cell model

Neumeister, Michael W.; Reichensperger, Joel; Yang, Ruizhi

doi:10.3892/ijo.2013.2039

Cited by 11 publications

(12 citation statements)

References 29 publications

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“…One century ago, Julius Cohnheim proposed that tumors are derived not from normal adult tissues, but from 'embryonal cell rests', which he described as residual embryonic cells 'left behind' in the adult organism (12). It was hypothesized that CSCs are important in tumor initiation, invasion, metastasis and resistance to anticancer therapies (13). A previous study demonstrated that stem cell-like cells can be isolated in vitro from gastric cancer tissues, which suggested that CSCs existed in gastric cancer (14).…”

Section: Discussionmentioning

confidence: 99%

Characterization of sphere-forming cells with stem-like properties from the gastric cancer cell lines MKN45 and SGC7901

Wang

Feng

Deng

et al. 2014

Molecular Medicine Reports

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Traditionally, it was presumed that gastric cancer was derived from tumor cells with stem‑like properties. In the present study, stem‑like cells from the gastric cancer cell lines MKN45 and SGC7901 were enriched by growing them as spheres in a defined serum‑free medium. Following enrichment for stem‑like cells, cluster of differentiation (CD)24 and CD44 were applied as candidate stem cell markers to examine the expression profile. It was revealed that the sphere‑derived cells contained a higher proportion of cells expressing the stem cell surface markers CD24 and CD44 when compared with the parental cells. It was also identified that the expression of cytokeratin 18 in sphere‑derived cells was decreased and the expression of vimentin and aldehyde dehydrogenase 1 (ALDH1) was increased compared with the parental cells. This finding supports the existence of a population of tumor sphere‑forming cells with stem cell properties in the MKN45 and SGC7901 cell lines. Furthermore, the stem cell population was enriched in cells expressing CD24, CD44, vimentin and ALDH1 cell surface markers. These results support the existence of gastric cancer stem cells and provide an alternative approach to the diagnosis and treatment of gastric cancer.

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Section: Discussionmentioning

confidence: 99%

Characterization of sphere-forming cells with stem-like properties from the gastric cancer cell lines MKN45 and SGC7901

Wang

Feng

Deng

et al. 2014

Molecular Medicine Reports

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“…Some lncRNAs have been suggested for use in clinical applications, such as diagnosis, prognosis, and treatment. 103 However, the relationship between lncRNAs and GC has only recently begun to be studied in detail.…”

Section: Future Directionsmentioning

confidence: 99%

Long noncoding RNAs in gastric cancer: functions and clinical applications

Wang¹,

Sun²,

Wang³

et al. 2016

OTT

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Over the last two decades, genome-wide studies have revealed that only a small fraction of the human genome encodes proteins; long noncoding RNAs (lncRNAs) account for 98% of the total genome. These RNA molecules, which are >200 nt in length, play important roles in diverse biological processes, including the immune response, stem cell pluripotency, cell proliferation, apoptosis, differentiation, invasion, and metastasis by regulating gene expression at the epigenetic, transcriptional, and posttranscriptional levels. However, the detailed molecular mechanisms underlying lncRNA function are only partially understood. Recent studies showed that many lncRNAs are aberrantly expressed in gastric cancer (GC) tissues, gastric juice, plasma, and cells, and these alterations are linked to the occurrence, progression, and outcome of GC. Here, we review the current knowledge of the biological functions and clinical aspects of lncRNAs in GC.

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“…This causes the emergence of metaplasia and dysplasia, and additional mutations which may lead to the development of cancer stem cells and appearance of malignant tumors [4]. We also know that cancer stem cells or circulating tumor cells play an important role in tumor invasion and resistance to anticancer therapy [20]. There is evidence that bone marrow stem cells may be involved in the development of carcinomas of the upper gastrointestinal tract -the esophagus and stomach [32].…”

Section: Cell Therapy In the Treatment Of Digestive Tract Malignanciesmentioning

confidence: 99%

“…There is evidence that bone marrow stem cells may be involved in the development of carcinomas of the upper gastrointestinal tract -the esophagus and stomach [32]. Therefore, to date, inhibition of gastric cancer stem cells is considered as one of the mechanisms of anticancer therapy [20,36].…”

Section: Cell Therapy In the Treatment Of Digestive Tract Malignanciesmentioning

confidence: 99%

“…In recent years, considerable attention of scientists is directed to the investigation of biological characteristics of digestive tract stem cells: their involvement in the regeneration of the mucous layer of the stomach and intestines, liver, as well as in the processes of malignant transformation and metastasis [4,20,22,29,36]. Equally important is the subject of mobilization and possible involvement of circulating bone marrow stem cells in the regeneration and carcinogenesis of the digestive tract [26,33].…”

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confidence: 99%

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