Therapeutic Effects of Topical Application of Ozone on Acute Cutaneous Wound Healing

Abstract

This study was undertaken to evaluate the therapeutic effects of topical ozonated olive oil on acute cutaneous wound healing in a guinea pig model and also to elucidate its therapeutic mechanism. After creating full-thickness skin wounds on the backs of guinea pigs by using a 6 mm punch biopsy, we examined the wound healing effect of topically applied ozonated olive oil (ozone group), as compared to the pure olive oil (oil group) and non-treatment (control group). The ozone group of guinea pig had a significantly smaller wound size and a residual wound area than the oil group, on days 5 (P<0.05) and 7 (P<0.01 and P<0.05) after wound surgery, respectively. Both hematoxylin-eosin staining and Masson-trichrome staining revealed an increased intensity of collagen fibers and a greater number of fibroblasts in the ozone group than that in the oil group on day 7. Immunohistochemical staining demonstrated upregulation of platelet derived growth factor (PDGF), transforming growth factor-β (TGF-β) and vascular endothelial growth factor (VEGF) expressions, but not fibroblast growth factor expression in the ozone group on day 7, as compared with the oil group. In conclusion, these results demonstrate that topical application of ozonated olive oil can accelerate acute cutaneous wound repair in a guinea pig in association with the increased expression of PDGF, TGF-β, and VEGF.

Aqueous-ozone irrigation of bone monocortical wounds in hyperglycemic rats

“…Previous investigations reported that ozone interactions with organic fluids stimulates vascular neoformation through a ROS mediates mechanism 11 and improves inflammatory response within pro-inflammatory cytokines interleukin-1 (IL-1) and factor-α tumor necrosis (TNF-α) release 12 .…”

Effect of ozone and methylprednisolone treatment following crush type sciatic nerve injury

“…It has been used as a therapeutic agent for chronic and diabetic wounds 7 . Ozone exerts its beneficial effects by supression of infection, regulation of inflammation and increasing local oxygen tension at the site of injury 8,9 . Ozone is particularly useful for infected and necrotic wounds and poorly oxygenated tissues 6 .…”

Effects of topical application of pure and ozonized andiroba oil on experimentally induced wounds in horses

“…Following this line of thought, it was expected that a greater contraction degree be seen in the GO wounds of the present study, but such was not observed. It is noteworthy that in this group ozone was mixed with NaCl 0.9% solution and it is well known that ozone maintains its active stage for a longer period of time when mixed with oils (KIM et al, 2009), being unstable in aqueous solutions. Additionally, different ozone concentration can influence results and optimal concentrations have to be established, because like any other chemical substance, insufficient doses may not produce satisfactory results and excessive doses may be harmful.…”

“…More recent therapeutic protocols recommend combination of vegetable oils with medical ozone for the treatment of wounds due to its oxidant properties, its bactericidal and bacteriostatic effects, and its ability to promote granulation tissue formation (KIM et al, 2009). Therapeutic effects of topical ozone application have been studied in the healing skin of guinea pigs (Cavia porcellus) (KIM et al, 2009), which demonstrated that presence of ozone accelerated skin repair.…”

“…Therapeutic effects of topical ozone application have been studied in the healing skin of guinea pigs (Cavia porcellus) (KIM et al, 2009), which demonstrated that presence of ozone accelerated skin repair. Similarly, ozonized sesame oil at a concentration of 1631 ± 64 mEq/1000 was able to accelerate the healing process in the initial phase (VALACCHI et al, 2010).…”