Effects of topical application of pure and ozonized andiroba oil on experimentally induced wounds in horses

Abstract

The aim of this study was to evaluate clinical and histopathological aspects of topical application of pure and ozonized andiroba oil (Carapa guianensis Aublet.) on the healing process of wounds in healthy horses. Eight 6.25 cm 2 wounds were surgically produced on each horse, from the cranial region to the sacrum, being four wounds on each side of the lumbar region. In three animals, left side was used for macroscopic observations and area measurement and right side was used for histopathological analysis. For the other two animals, evaluations were inverted. The beginning of the topical treatment occurred 12 hours after surgical induction of the injuries and was maintained daily until complete healing of the wounds, using saline solution (GC), ozonized saline solution (GO) sequentially and bilaterally in the craniocaudal direction, pure andiroba oil (GAP) and ozonized andiroba oil (GAO). Randomly, the sequence of the treatments was modified. Macroscopic and histopathological analyses were performed at 3, 7, 14, and 21 days after surgery. The time for complete healing of all wounds was recorded. A wound contraction of 67.75% for GC, 65.26% for GO, 67.91% for GAP, and 69.84% for GAO were recorded. Histopathologic evaluation revealed that wounds from the GAO and GAP had an advanced epithelialization, fibroblast proliferation and collagen deposition, moderate vascular proliferation, and presence of PMN infiltrate and discrete viewing of MN. It was possible to conclude that all treatments had benefits when comparing to control group, concluding that both pure and ozonized andiroba oil may be good options for treating wounds in horses. Keywords: Carapa guianensis. Healing process. Equines. Wounds. Ozone. ResumoEste trabalho realizou uma avaliação clínica e histopatológica da aplicação tópica do óleo de andiroba (Carapa guianensis Aublet), puro e ozonizado, no processo de cicatrização de feridas em cinco equinos saudáveis. Oito feridas de 6,25 cm 2 foram induzidas cirurgicamente, quatro de cada lado da região lombar, craniais em relação à região sacral. Em três animais, o lado esquerdo foi destinado à avaliação macroscópica e mensuração de área, enquanto o lado direito foi destinado à análise histopatológica. Nos outros dois animais, as avaliações foram invertidas. O tratamento tópico foi iniciado 12 horas após a indução cirúrgica e foi mantido diariamente até a completa cicatrização das feridas. Foram usados, sequencialmente e bilateralmente, no sentido craniocaudal: solução salina (GC), solução salina ozonizada (GO), óleo de andiroba puro (GAP) e óleo de andiroba ozonizado (GAO). Aleatoriamente, a sequência de tratamentos foi modificada. As análises macroscópicas e microscópicas foram realizadas 3, 7, 14 e 21 dias após a cirurgia, e o tempo total para cicatrização registrado. A contração da ferida foi de 67,75% para GC, 65,26% para GO, 67,91% para GAP, e 69,84% para GAO. A avaliação histopatológica demonstrou que as feridas tratadas com GAO e GAP apresentaram uma avançada epitelização, proliferação fibroblás...

Liquid ozone therapies for the treatment of epithelial wounds: A systematic review and meta‐analysis

“…Ten studies examined the impact of ozonated oils/water on wound size. (Table 2 ) Three studies were within‐subject trials of ozonated oils/water compared to control or standard treatment 19 , 20 , 21 and seven studies were between‐subjects trials of ozonated oils and/or ozonated water compared to control or standard treatments. 22 , 23 , 24 , 25 , 26 , 27 , 28 …”

“…37 Two studies on horses found inconsistent results. 19 , 20 A study of eight horses, each with eight wounds, found wounds treated with ozonated sunflower oil reached complete healing faster than wounds treated with pure oil or saline. 20 A similar study of five horses, each with four wounds, found time to complete healing in the wounds treated with ozonated andiroba oil or pure andiroba oil were healed on day 30, wounds treated with ozonated saline were healed on day 32, and wounds in the control group were healed on day 31.…”

“… 20 A similar study of five horses, each with four wounds, found time to complete healing in the wounds treated with ozonated andiroba oil or pure andiroba oil were healed on day 30, wounds treated with ozonated saline were healed on day 32, and wounds in the control group were healed on day 31. 19 …”

“…The remaining 13 studies had a mixture of low risk and unclear risk ratings. 19 , 21 , 24 , 25 , 26 , 27 , 29 , 30 , 33 , 35 , 36 , 37 , 40 …”

Cicatrização de ferida profunda em equino com uso de dreno ativo, laser de baixa potência e ultrassom terapêutico

“…Fatores como o comportamento intempestivo frente à manipulação, ou mesmo devido à presença de materiais pontiagudos ou cortantes e instalações inadequadas nos locais de manejo, predispõe à ocorrência de lesões de pele (Viana et al, 2014). Os cavalos possuem características particulares frente aos processos orgânicos de cicatrização de feridas, despertando um importante interesse clínico, científico e econômico sobre o assunto (Araujo et al, 2017). Nas feridas tratadas por segunda intenção, são frequentemente observadas alterações como a contaminação da lesão, com produção de conteúdo purulento, cicatrização mais lenta em comparação às outras espécies, além da formação excessiva de tecido de granulação.…”