Therapeutic effect of simvastatin on DMBA‐induced breast cancer in mice

Karimi, Behrooz; Ashrafi, Mahboobeh; Shomali, Tahoora; Yektaseresht, Azadeh

doi:10.1111/fcp.12397

Cited by 33 publications

(27 citation statements)

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“…It is recorded that the tumor size significantly decreased after 16 days of treatment (707 mm³ vs. 825 mm³ in control, Pvalue=0.01) compared to the control. These findings approved antitumor effect of simvastatin on solid tumor animal model and agreed with the finding on a rat model of malignant glioma [26] and breast cancer in the mice [27]. Simvastatin recorded good results of ILS% (31.58), T/C (132) and TIR% is 14.28%.…”

Section: Discussionsupporting

confidence: 88%

Anti-Tumor Effect of Green Tea Extract, Simvastatin and Gamma Radiation on Solid Tumor in Mice

Abdelrahman

El‐Kashef

Hassan

2020

Arab Journal of Nuclear Sciences and Applications

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The current study was conducted of six groups (n=12) to define the efficacy of epigallocatechin gallate, simvastatin and gamma radiation individually or combined together to reduce solid tumor size induced by intramuscular injection of EC cells in the right thigh of the targeted mice. Magnetic resonance imaging device)1.5 Tesla(used for radiology imaging of the normal and ascites-bearing mice under anesthesia. The animals with solid tumors were followed up daily for recording deaths, tumor size, rate of inflammation and gangrene in the tumor area of hind limb. Magnetic resonance imaging showed the intraperitoneal ascites and the soft organs (liver, kidney and intestine) which were bright in the case of ascites-bearing mouse than the normal control. Triple therapy regimen significantly reduced the tumor size (662.6±12.5) compared to the positive control (825±21.8) and increased survival rate to 66.7% compared to 25% in the positive control. Triple therapy reduced the inflammation of tumor area, preventing the gangrene incidence, and showed the best value of T/C=142, ILS=42.11 and TIR %=19.6 compared to the positive control and single treatments, hence, it is considered an anti-tumor regimen according to the National Cancer Institute criteria.

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Section: Discussionsupporting

confidence: 88%

Anti-Tumor Effect of Green Tea Extract, Simvastatin and Gamma Radiation on Solid Tumor in Mice

Abdelrahman

El‐Kashef

Hassan

2020

Arab Journal of Nuclear Sciences and Applications

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“…DMBA promotes the production of prostaglandin E2 (PGE2) which in turn increase the incidence of mammary tumors . In addition to other carcinogenic properties as mutation and disturbance of cellular oxidant-antioxidant balance (9).…”

Section: Introductionmentioning

confidence: 99%

Green nanogold activity in experimental breast carcinoma in vivo

et al. 2020

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Background: Over the past few years, fabrication of nanoparticles (NPs) has been deployed widely in technologies and many concerns have emerged about the hazardous effect on human health after NPs exposure. Objective: Green synthesis of gold NPs (AuNPs) and assessment of their activity in 7,12-dimethylbenz(a)anthracene (DMBA)-induced breast cancer mouse model. Methods: Chloroauric acid (HAuCl4) was used in formation of AuNPs with the help of Curcuma longa as aqueous reducing extract and stabilizing agent at room temperature. Formed NPs were characterized with UV-Vis spectrometry, Fourier-transform infrared spectroscopy (FTIR), Zetasizer measurement, Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). Virgin female albino mice with DMBA-induced breast cancer were treated with formed AuNPs for 5 consecutive days and were dissected after 28 days of the beginning of treatment. Results: UV-Vis spectrometry showed absorbance maximum peak at 530 nm for formed AuNPs, FTIR confirmed formation of plant extract layer around formed NPs; zetasizer measurement revealed 278.2 nm as an average size of produced NPs; SEM and TEM approved formation of monodisperse spherical AuNPs. Biochemical analysis of untreated breast cancer group revealed marked changes in liver and kidney functions manifested by raised activity levels of alanine transaminase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine. Whereas, the treated group with AuNPs post-breast cancer induction displayed reduction in the activities (of ALT, AST and creatinine), while the BUN activity level was raised. Histopathological examination showed heavy incidence of tumor foci in the breast and lymph nodes belonged to the untreated breast cancer group confirmed with intense response to Ki-67 antibodies. While the treated group with AuNPs post-breast cancer induction showed degenerated tumor foci in the breast and lymph nodes with weak response to Ki-67 antibodies. Conclusion: AuNPs were successfully synthesized using HAuCl4 and C. longa extract confirmed their ability to control DMBA-induced breast cancer in virgin female Swiss albino mice.

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“…The mice were weighed and palpated weekly for the appearance of mammary tumors 4 weeks after DMBA administration till to the end of the experiment. When tumors size reached 0.5cm in the largest dimension, animals in group D were divided into three groups, 7 in each group (Karimi et al, 2018). Groups were labeled as follows: C, tumoric mice without treatment which were represented as the control group (n=7); S, tumeric mice with simvastatin treatment that received it by gavage with a dose of 80 mg/kg/day for 4 consecutive weeks (n=7) and T: tumeric mice with tamoxifen treatment that received it by gavage with a dose of 50 mg/kg/day for 4 consecutive weeks (n=7).…”

Section: Animals and Experimental Designmentioning

confidence: 99%