Therapeutic benefit of Muse cells in a mouse model of amyotrophic lateral sclerosis

Yamashita, Toru; Kushida, Yoshihiro; Wakao, Shohei; Tadokoro, Koh; Nomura, Emi; Omote, Yoshio; Hishikawa, Nozomi; Ohta, Yasuyuki; Dezawa, Mari

doi:10.1038/s41598-020-74216-4

Cited by 30 publications

(28 citation statements)

References 40 publications

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“…So far, Muse cells have been used in neurological disorders [55][56][57][58], acute myocardial infarction [59], adriamycin nephropathy [60], diabetic skin ulcers [61], and liver fibrosis [35]. In these disease models, Muse cells could migrate to lesion sites and spontaneously differentiate into tissue-compatible cells, such as neurons (ectoderm) [6,12,[55][56][57][58]62], cardiac cells (mesoderm) [59], glomerular cells (mesoderm) [60], vascular endothelial cells (mesoderm) [61] and hepatocytes (entoderm) [35]. Actually, although the differentiation rate is generally very low, Muse cells have spontaneous triploblastic differentiation ability in vitro [26,62].…”

Section: Differentiation Potentialmentioning

confidence: 99%

“…The most important thing is to establish a standard criterion for the sorting methods of Muse cells. Fluorescence-activated cell sorting (FACS) [26,55,63], magnetic-activated cell sorting (MACS) [28,61,84], and long-term trypsin incubation (LTT) [85-87] are three commonly used methods to achieve Muse cells. Uchida et al [56] suggested Muse cells isolated by MACS had higher cell viability and the collection efficiency was better compared with FACS.…”

Section: Obstacles and Challenges Of Muse Cells From Bench To Bedsidementioning

confidence: 99%

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Muse cells: ushering in a new era of stem cell-based therapy for stroke

Wei

Liu

et al. 2022

Stem Cell Res Ther

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Stem cell-based regenerative therapies have recently become promising and advanced for treating stroke. Mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs) have received the most attention for treating stroke because of the outstanding paracrine function of MSCs and the three-germ-layer differentiation ability of iPSCs. However, the unsatisfactory homing ability, differentiation, integration, and survival time in vivo limit the effectiveness of MSCs in regenerative medicine. The inherent tumorigenic property of iPSCs renders complete differentiation necessary before transplantation, which is complicated and expensive and affects the consistency among cell batches. Multilineage differentiating stress-enduring (Muse) cells are natural pluripotent stem cells in the connective tissues of nearly every organ and thus are considered nontumorigenic. A single Muse cell can differentiate into all three-germ-layer, preferentially migrate to damaged sites after transplantation, survive in hostile environments, and spontaneously differentiate into tissue-compatible cells, all of which can compensate for the shortcomings of MSCs and iPSCs. This review summarizes the recent progress in understanding the biological properties of Muse cells and highlights the differences between Muse cells and other types of stem cells. Finally, we summarized the current research progress on the application of Muse cells on stroke and challenges from bench to bedside.

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Section: Differentiation Potentialmentioning

confidence: 99%

Section: Obstacles and Challenges Of Muse Cells From Bench To Bedsidementioning

confidence: 99%

Muse cells: ushering in a new era of stem cell-based therapy for stroke

Wei

Liu

et al. 2022

Stem Cell Res Ther

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“…Several animal studies have shown the safety and effectiveness of human Muse cells for treating neurological disorders, such as stroke, intracerebral hemorrhage, encephalopathy, ALS, and SCI (see Table S6 ). Moreover, Muse cells can engraft and integrate into the damaged regions and differentiate into neuronal cells, and finally, lead to functional and morphological recovery after intravenous administration [ 167 , 168 , 169 , 170 ]. However, Muse cells are still a novel type of stem cells that have not been well studied as other stem cells.…”

Section: Different Cell Types and The Current Progressmentioning

confidence: 99%

Cell Therapy for Neurological Disorders: The Perspective of Promising Cells

Liu

Bobrovskaya

Zhou

2021

Biology

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Neurological disorders are big public health challenges that are afflicting hundreds of millions of people around the world. Although many conventional pharmacological therapies have been tested in patients, their therapeutic efficacies to alleviate their symptoms and slow down the course of the diseases are usually limited. Cell therapy has attracted the interest of many researchers in the last several decades and has brought new hope for treating neurological disorders. Moreover, numerous studies have shown promising results. However, none of the studies has led to a promising therapy for patients with neurological disorders, despite the ongoing and completed clinical trials. There are many factors that may affect the outcome of cell therapy for neurological disorders due to the complexity of the nervous system, especially cell types for transplantation and the specific disease for treatment. This paper provides a review of the various cell types from humans that may be clinically used for neurological disorders, based on their characteristics and current progress in related studies.

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“…Based on these unique characteristics, intravenously administered allogenic Muse cells are already widely used in clinical trials [ 10 ]. At present, studies on Muse cells have focused on their differentiation ability, which is regarded as a new development direction for stem cell therapy [ 11 – 15 ]. However, the therapeutic efficacy of Muse cells in AD has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Multilineage-differentiating stress-enduring cells alleviate atopic dermatitis-associated behaviors in mice

WenDi

Gao

et al. 2021

Stem Cell Res Ther

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Background Pruritus is a recurring, long-lasting skin disease with few effective treatments. Many patients have unsatisfactory responses to currently available antipruritic treatments, and effective therapeutics are urgently needed to relieve symptoms. A previous study reported that mesenchymal stem cell (MSC)-mediated immune regulation could be used to treat skin inflammatory diseases. Multilineage-differentiating stress-enduring (Muse) cells are a new type of pluripotent stem cell that may also have the potential to treat inflammatory skin diseases. Methods Muse cells were isolated from human bone marrow-derived MSCs (BMSCs) via the 8-h longterm trypsin incubation (LTT) method. Repeated use of 2,4-dinitrofluorobenzene (DNFB) induced atopic dermatitis (AD) in a mouse model. Immunofluorescence, behavior recording, and image analysis were used to evaluate the therapeutic effect of subcutaneous Muse cell injection. Real-time quantitative polymerase chain reaction (qPCR) was used to measure the expression of inflammatory factors. In vitro, wound healing and cell proliferation experiments were used to examine the effect of Muse cell supernatant on keratinocytes. Results Our results showed that subcutaneous injection of Muse cells after AD model induction significantly alleviated scratching behavior in mice. The evaluation of dermatitis and photos of damaged skin on the back of the neck revealed that Muse cells reduced dermatitis, playing an active role in healing the damaged skin. The activation of spinal glial cells and scratching behavior were also reduced by Muse cell injection. In addition, we also showed that the expression levels of the inflammatory factors interleukin (IL)-6, IL-17α, and IL-33 in both the spinal cord and skin were suppressed by Muse cells. Furthermore, Muse cells not only exerted anti-inflammatory effects on lipopolysaccharide (LPS)-induced human HaCat cells but also promoted wound healing and keratinocyte proliferation. Conclusions In vivo, Muse cells could alleviate scratching symptoms, reduce epidermal inflammation, and promote wound healing. In vitro, Muse cells could also promote the migration and proliferation of keratinocytes. In summary, Muse cells may become a new therapeutic agent for the treatment of AD.

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Therapeutic benefit of Muse cells in a mouse model of amyotrophic lateral sclerosis

Cited by 30 publications

References 40 publications

Muse cells: ushering in a new era of stem cell-based therapy for stroke

Muse cells: ushering in a new era of stem cell-based therapy for stroke

Cell Therapy for Neurological Disorders: The Perspective of Promising Cells

Multilineage-differentiating stress-enduring cells alleviate atopic dermatitis-associated behaviors in mice

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