Theoretical and experimental studies on sequential two-diffusional sample injection for capillary electrophoresis

Liu, Lina; Tian, Miaomiao; Liu, Xiaoxia; Guo, Liping; Yang, Li

doi:10.1016/j.chroma.2015.01.006

Cited by 4 publications

(6 citation statements)

References 32 publications

(40 reference statements)

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“…A theoretical model of a sequential diffusion injection system has been designed for 2D diffusion . Based on the Fick's second law, two capillaries are aligned coaxially with an imposed controlled distance (15 μm) and pass through a sample vial.…”

Section: New Developments In Cementioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

et al. 2015

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We describe the most important research articles published on amino acid analysis using CE during the period from June 2013 to May 2015, and follows the format of the previous articles published in electrophoresis the new developments in amino acid analysis with CE are mainly describing improvements in detection means and injection methods. Enantiomeric separation developments are still important. Focusing the applications, we describe the neurochemical and clinical works, but also the metabolomic studies for which the publication number increase greatly. Finally, works focused on amino acids in food and agricultural applications are described.

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Section: New Developments In Cementioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

et al. 2015

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“…Twelve articles were considered from this mode . Mesbah et al.’s strategy for in‐capillary labelling of nanomolar‐level ubiquitin in cerebrospinal fluid with Fluoprobes® 488 N ‐hydroxysuccinimide is shown in Fig.…”

Section: Derivatisation Modesmentioning

confidence: 99%

“…Yang and co‐workers showed that derivatisation was beneficial for sequential diffusion injection of DNP, 2‐mercaptoethanol or OPA‐labelled amino acids since this reaction step improved the diffusion rate by changing the concentration gradient . Sequential injection methodology is important for on‐line monitoring of processes because it provides vital information in many time‐dependent biotechnological processes (i.e.…”

Section: Derivatisation For Detectionmentioning

confidence: 99%

“…Derivatisation in CE is used to improve or enable detection by LIF, UV, chemiluminescence (CL), electrochemiluminescence (ECL), amperometric detection (AD), MS or contactless conductivity detection (C4D). During this review period, there were papers 18 on LIF (and LIF combined with other detectors) [, , ], 18 on UV (and UV combined with other detectors) [, , ], two on CL , one on ECL , two on AD , three on MS (and MS combined with other detectors) and two on C4D .…”

Section: Derivatisation For Detectionmentioning

confidence: 99%

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Derivatisation for separation and detection in capillary electrophoresis (2012–2015)

Wuethrich

Quirino

2015

Electrophoresis

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Derivatisation is a well-established and mature form of sample preparation for CE. The modification of the analyte can cause superior analysis characteristics such as better sensitivity and selectivity, however, derivatisation of the analyte introduces an additional step into the analytical workflow. This review covers articles from January 2012 to January 2015 on derivatisation in CE. The main sections are on the derivatisation modes (i.e. pre-capillary, in-line, in-capillary and post-capillary), separation and detection modes (i.e. LIF and others). LIF is discussed in more detail since this detection mode was most prevalent. A table of the common labelling agents and wavelengths for excitation and emission and the common derivatisation reactions are included. In addition, a comprehensive table which summarises all research articles is provided. This review is suitable for analytical chemists as a guide for 'how to get started' with derivatisation for separation and detection in CE.

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“…The resulting product(s) and remaining reactants are electrophoretically separated in the downstream of the capillary for quantitative determination of the enzyme activity. Additionally, sequential CE enzyme assays have been developed by periodical and automatic injection of the reaction mixture into the capillary via either optical‐gated photobleaching injection or two‐dimension diffusion injection .…”

Section: Introductionmentioning

confidence: 99%

Enzyme and inhibition assay of urease by continuous monitoring of the ammonium formation based on capillary electrophoresis

et al. 2016

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We present here an easy-to-operate and efficient method for enzyme and inhibition assays of urease, which is a widely distributed and important enzyme that catalyzes the hydrolysis of urea to ammonia and CO . The assay was achieved by integrating CE technique and rapid on-line derivatization method, allowing us to continuously drive the sample to the capillary, thus to measure the amount of the product ammonia from the beginning to the end of the reaction. The method exhibits excellent repeatability with RSD as low as 2.5% for the initial reaction rate (n = 5), with the LOD of ammonia of 20 μM (S/N = 5). The enzyme activity as well as the inhibition of urease by Cu were investigated using the present method. The results show that Cu is a noncompetitive inhibitor on urease, in accordance with the result published in the literature. The enzyme activity and inhibition kinetic constants were obtained and were found to be consistent with the results of traditional off-line enzyme assays. Our study indicates that the present approach is a reliable and convenient method for analysis of the urease activity and inhibition kinetics by continuous on-line monitoring of the ammonium formation based on CE.

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Theoretical and experimental studies on sequential two-diffusional sample injection for capillary electrophoresis

Cited by 4 publications

References 32 publications

Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

Derivatisation for separation and detection in capillary electrophoresis (2012–2015)

Enzyme and inhibition assay of urease by continuous monitoring of the ammonium formation based on capillary electrophoresis

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