Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

Abstract

We describe the most important research articles published on amino acid analysis using CE during the period from June 2013 to May 2015, and follows the format of the previous articles published in electrophoresis the new developments in amino acid analysis with CE are mainly describing improvements in detection means and injection methods. Enantiomeric separation developments are still important. Focusing the applications, we describe the neurochemical and clinical works, but also the metabolomic studies for which the publication number increase greatly. Finally, works focused on amino acids in food and agricultural applications are described.

Noteworthy issues for producing and transforming bioproducts by electrodalysis

“…They have wide applications in the fields of pharmaceuticals, foodstuff, chemical industry, agriculture, environment protection, and so on. 81,82 Among more than 300 kinds of amino acid in nature, methionine is the only one containing elemental sulfur. It can promote growth and improve the grade of meat and is thus often used as a feed additive.…”

Capillary electrophoresis determination of non-protein amino acids as quality markers in foods

“…Therefore, taking into account that assuring product food quality, authenticity, and safety is the main demand in the food field, there is an increasing need of analytical methodologies enabling the determination of non-protein amino acids in foodstuffs. Among the advanced analytical techniques that can be used to solve some of the challenges that Food Science has to face, Capillary Electrophoresis (CE) has already demonstrated its high potential for the (achiral and chiral) determination of many compounds in foods, including amino acids, to ensure compliance with food and trade laws [1,16,19,20]. Among the CE modes used to determine non-protein amino acids in food samples, CZE (based on the free mobility of analytes in the aqueous solution under an applied electric field) and MEKC (based on the combination of electrokinetic migration and the partitioning mechanism between the bulk solution and micelles) are the most employed, whereas EKC and CEC (based on the interaction of each enantiomer with a chiral selector present in the mobile phase or with a chiral stationary phase, respectively) are the most employed CE modes to achieve an enantiomeric separation.…”

Free amino acids, biogenic amines, and ammonium profiling in tobacco from different geographical origins using microwave‐assisted extraction followed by ultra high performance liquid chromatography

“…Amino acids, biogenic amines, and ammonium are usually analyzed by TLC, GC, CE, IC, and HPLC separation techniques . Of these techniques, HPLC is the most popular and useful method for the simultaneous analysis of these compounds.…”