The wheat E3 ligase TaPUB26 is a negative regulator in response to salt stress in transgenic Brachypodium distachyon

Wu, Yunzhen; Wang, Wenlong; Li, Qinxue; Zhang, Guangqiang; Zhao, Xiaoyu; Li, Genying; Li, Yulian; Wang, Yong

doi:10.1016/j.plantsci.2020.110441

Cited by 19 publications

(16 citation statements)

References 86 publications

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“…As reported in previous studies, overexpressing PUB genes negatively regulate the drought and salt stress response in some plant species, such as PUB11, PUB22, PUB23, and AtPUB30 in Arabidopsis [31,32,40], OsPUB41 and OsPUB75 in rice [24,44], GmPUB6 and GmPUB8 in soybean [27,34], and TaPUB26 in wheat [45]. In our study, overexpressing GmPUB21 shows sensitive to drought, salinity stress and ABA treatment (Figure 3), and more ROS (O 2 − and H 2 O 2 ) accumulated in transgenic plants after drought and salinity treatment, which may disturb the tolerance to drought and salt stress.…”

Section: Discussionsupporting

confidence: 58%

The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

Yang

Karthikeyan

Yin

et al. 2022

IJMS

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E3-ubiquitin ligases are known to confer abiotic stress responses in plants. In the present study, GmPUB21, a novel U-box E3-ubiquitin ligase-encoding gene, was isolated from soybean and functionally characterized. The expression of GmPUB21, which possesses E3-ubiquitin ligase activity, was found to be significantly up-regulated by drought, salinity, and ABA treatments. The fusion protein GmPUB21-GFP was localized in the cytoplasm, nucleus, and plasma membrane. Transgenic lines of the Nicotiana benthamiana over-expressing GmPUB21 showed more sensitive to osmotic, salinity stress and ABA in seed germination and inhibited mannitol/NaCl-mediated stomatal closure. Moreover, higher reactive oxygen species accumulation was observed in GmPUB21 overexpressing plants after drought and salinity treatment than in wild-type (WT) plants. Contrarily, silencing of GmPUB21 in soybean plants significantly enhanced the tolerance to drought and salinity stresses. Collectively, our results revealed that GmPUB21 negatively regulates the drought and salinity tolerance by increasing the stomatal density and aperture via the ABA signaling pathway. These findings improved our understanding of the role of GmPUB21 under drought and salinity stresses in soybean.

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Section: Discussionsupporting

confidence: 58%

The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

Yang

Karthikeyan

Yin

et al. 2022

IJMS

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“…In contrast, TaPUB26 acts as a negative regulator of salt stress tolerance [ 143 ]. Here, stiff brome ( Brachypodium distachyon ) transgenic lines overexpressing this E3 ligase exhibited higher sensitivity toward salinity, which was accompanied by accumulation of ROS, and an increased Na + /K + ratio [ 143 ]. TaPUB26 interacts with T. aestivum REGULATORY PARTICLE AAA-ATPASE 2A (TaRPT2a), which functions as an ATPase subunit of the 26S proteasome complex.…”

Section: Salt Stressmentioning

confidence: 99%

“…TaPUB26 interacts with T. aestivum REGULATORY PARTICLE AAA-ATPASE 2A (TaRPT2a), which functions as an ATPase subunit of the 26S proteasome complex. Its gene expression is salt inducible, however the mechanism of how TaPUB26-TaRPT2a interaction affects salt stress tolerance is still not understood [ 143 ].…”

Section: Salt Stressmentioning

confidence: 99%

Plant E3 Ligases and Their Role in Abiotic Stress Response

Al-Saharin

Hellmann

Mooney

2022

Cells

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Plants, as sessile organisms, have limited means to cope with environmental changes. Consequently, they have developed complex regulatory systems to ameliorate abiotic stresses im-posed by environmental changes. One such system is the ubiquitin proteasome pathway, which utilizes E3 ligases to target proteins for proteolytic degradation via the 26S proteasome. Plants ex-press a plethora of E3 ligases that are categorized into four major groups depending on their structure. They are involved in many biological and developmental processes in plants, such as DNA repair, photomorphogenesis, phytohormones signaling, and biotic stress. Moreover, many E3 ligase targets are proteins involved in abiotic stress responses, such as salt, drought, heat, and cold. In this review, we will provide a comprehensive overview of E3 ligases and their substrates that have been connected with abiotic stress in order to illustrate the diversity and complexity of how this pathway enables plant survival under stress conditions.

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“…TaPUB26 is induced by high salinity, cold, drought and phytohormones. Ectopic expression of TaPUB26 in Brachypodium distachyon leads to a hypersensitive phenotype to salt stress, manifested by reduced chlorophyll contents, decreased photosynthetic capabilities and antioxidant enzyme activities, more ROS accumulation and severer cell membrane damage relative to the WT plants [50]. Moreover, the transgenic plants have higher Na + contents and lower K + contents following salt treatment, hence maintain a higher cytosolic Na + /K + ratio in plant cells.…”

Section: Pubs Are Key Components In the Response To Salt Stressmentioning

confidence: 99%

How Many Faces Does the Plant U-Box E3 Ligase Have?

Mao

et al. 2022

IJMS

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Ubiquitination is a major type of post-translational modification of proteins in eukaryotes. The plant U-Box (PUB) E3 ligase is the smallest family in the E3 ligase superfamily, but plays a variety of essential roles in plant growth, development and response to diverse environmental stresses. Hence, PUBs are potential gene resources for developing climate-resilient crops. However, there is a lack of review of the latest advances to fully understand the powerful gene family. To bridge the gap and facilitate its use in future crop breeding, we comprehensively summarize the recent progress of the PUB family, including gene evolution, classification, biological functions, and multifarious regulatory mechanisms in plants.

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The wheat E3 ligase TaPUB26 is a negative regulator in response to salt stress in transgenic Brachypodium distachyon

Cited by 19 publications

References 86 publications

The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

Plant E3 Ligases and Their Role in Abiotic Stress Response

How Many Faces Does the Plant U-Box E3 Ligase Have?

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