The value of rRNA gene restriction site polymorphism analysis for delineating taxa in the genus Staphylococcus.

Abstract

A total of 101 staphylococcal strains were ribotyped using EcoRI and HindIII as restriction enzymes and plasmid pBA2 as the rDNA probe. Isolates from 10 newly described staphylococcal taxa were among those examined. All the ribotypes were added to our database, Staph DB, which now contains the sizes of the bands of 135 EcoRI and 120 HindIII ribotypes from 408 strains belonging to 42 staphylococcal taxa. The relatedness of ribotypes was evaluated by using the Dice coefficient. The ribotypes, and thus the strains, were clustered by the unweighted pair group method with averages (UPGMA). Separation into clusters correlated well with the delineation of the staphylococcal species but not with that of the different subspecies. No discrimination was possible between Staphylococcus vitulinus and Staphylococcus pulvereri. Ecovarspecific groups were evident within Staphylococcus intermedius and Staphylococcus hyicus. The data increase the usefulness of rRNA gene restriction site polymorphism analysis for staphylococcal taxonomy.

Population Diversity of Staphylococcus intermedius Isolates from Various Host Species: Typing by 16S-23S Intergenic Ribosomal DNA Spacer Polymorphism Analysis

“…1), confirming the genomic diversity of S. intermedius strains observed elsewhere by using EcoRI ribotyping (1,7,16). All the human strains and 89% of the canine strains belonged to the same types (C and J), supporting the transmission of S. intermedius from dogs to humans.…”

Staphylococcus simiae sp. nov., isolated from South American squirrel monkeys

“…S2). This cluster comprising all of the novel isolates was clearly distinct from other reference Staphylococcus species at a level of similarity below 62 %, as used by Chesneau et al (2000) for separating different species of the genus Staphylococcus. Fig.…”

Prevalence and Characterization of Leukotoxin-Producing Staphylococcus intermedius in Isolates from Dogs and Pigeons

“…It has been shown by various genotyping methods, such as 16S-23S intergenic ribosomal DNA spacer polymorphism analysis (ITS-PCR), EcoRI ribotyping, and SmaI pulsed-field gel electrophoresis, that S. intermedius strains are diverse and that the genotypes of S. intermedius isolates from dogs are distinct from those from pigeons (2,3,18). The enterotoxins and hemolysins are more prevalent among S. intermedius isolates from dogs than among those from pigeons (5,17).…”