Population Diversity of
            <i>Staphylococcus intermedius</i>
            Isolates from Various Host Species: Typing by 16S-23S Intergenic Ribosomal DNA Spacer Polymorphism Analysis

Bes, Michèle; Slim, L.; Becharnia, Fatma; Meugnier, H.; Vandenesch, François; Étienne, Jérôme; Freney, J.

doi:10.1128/jcm.40.6.2275-2277.2002

Cited by 36 publications

(27 citation statements)

References 24 publications

(24 reference statements)

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“…Some reports suggested previously that SIG strains from horses were heterogenous (2,5,36). In the present study, both S. delphini group A and group B strains were isolated from horses.…”

Section: Discussionsupporting

confidence: 44%

“…With respect to nuc sequences in our results, these three groups might be S. pseudintermedius and S. delphini groups A and B, respectively. SIG strains from minks have been reported to be distinct from those from dogs by pulsed-field gel electrophoresis analyses and by their lack of thermonuclease production (5,6,36). In the present study, all SIG strains from dogs were identified as being S. pseudintermedius strains and were positive for thermonuclease production.…”

Section: Discussionmentioning

confidence: 44%

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Reclassification of Phenotypically Identified Staphylococcus intermedius Strains

et al. 2007

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To reclassify phenotypically identified Staphylococcus intermedius strains, which might include true S. intermedius strains and novel species such as Staphylococcus pseudintermedius and Staphylococcus delphini, we analyzed molecular phylogenies and phenotypic characteristics of 117 S. intermedius group (SIG) strains tentatively identified as being S. intermedius by the Rapid ID32 Staph assay. From phylogenetic analyses of sodA and hsp60 sequences, the SIG strains were divided into three clusters, which belonged to S. pseudintermedius LMG 22219 T , S. intermedius ATCC 29663 T , and S. delphini LMG 22190 T . All the SIG strains from dogs, cats, and humans were identified as being S. pseudintermedius. The wild pigeon strains, except one, were identified as being S. intermedius, and strains from all domestic pigeons, one wild pigeon, horses, and a mink were identified as being S. delphini. In addition, a phylogenetic analysis of nuc genes revealed that S. delphini strains were divided into two clusters: one was the cluster (S. delphini group A) that belonged to S. delphini LMG 22190 T , and the other was the cluster (S. delphini group B) that was more related to S. pseudintermedius LMG 22219 T than S. delphini LMG 22190 T . The DNA-DNA hybridization results showed that S. delphini group B strains were distinguished from S. delphini group A, S. intermedius, and S. pseudintermedius strains. S. intermedius is distinguishable from S. pseudintermedius or S. delphini by positive arginine dihydrolase and acid production from ␤-gentiobiose and D-mannitol. However, phenotypical characteristics to differentiate S. delphini group A, S. delphini group B, and S. pseudintermedius were not found. In conclusion, SIG strains were reclassified into four clusters with three established and one probably novel species.

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“…Some reports suggested previously that SIG strains from horses were heterogenous (2,5,36). In the present study, both S. delphini group A and group B strains were isolated from horses.…”

Section: Discussionsupporting

confidence: 44%

Section: Discussionmentioning

confidence: 44%

Reclassification of Phenotypically Identified Staphylococcus intermedius Strains

et al. 2007

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“…Meyer and Schleifer described how different biotypes of S. intermedius strains showed only 50 to 65% DNA homology using DNA-DNA reassociation tests, indicating that they belonged to different species (31). Other authors also reported that S. intermedius isolates from different sources could be genotypically or phenotypically differentiated (6,13,14). In the phylogenetic point of view, these results suggest that S. pseudintermedius is not a novel emerging species among dogs but might have been recognized as one of the different biotypes of S. intermedius.…”

Section: Discussionmentioning

confidence: 99%

Methicillin-ResistantStaphylococcus pseudintermediusin a Veterinary Teaching Hospital

et al. 2007

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“…S. intermedius, first described by Hajek (12), is the most common cause of skin infections in dogs, with canine pyoderma being caused almost exclusively by S. intermedius (16,37). It has also been isolated from a number of other carnivores, horses, ruminants and birds (7,13,18,22,23). In single cases, S. intermedius appears to be also responsible, as a zoonotic pathogen, for canine-inflicted human wound infections and invasive infections in immune compromised patients (28,31).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of exfoliative toxin in Staphylococcus intermedius isolates from dogs with pyoderma

MÜŞTAK¹

2013

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:Staphylococcus aureus and S. intermedius are considered as the most significant species causing skin infections in dogs. The aim of this study was to develop a Polymerase Chain Reaction technique for the detection of siet (S. intermedius exfoliative toxin) gene encoding exfoliative toxin in S. intermedius and to investigate its presence in S. intermedius isolates from dogs with pyoderma. A total of 41 isolates (35 S. intermedius, 4 S. aureus, one S. capitis subsp. ureolytica and one S. chromogenes) from dogs with pyoderma were included in the study. Original primers specifically amplifying 145 bp of siet gene and 182 bp of agr (accessory gene regulator) gene locus of S. intermedius were designed in the study. agr gene was detected in all S. intermedius isolates, but not in other isolates. siet gene was detected in all S. intermedius isolates. siet Polymerase Chain Reaction assay was found to be specific since no amplifications were observed with siet negative S. intermedius and other bacterial control strains. Rapid and reliable detection of staphylococci causing skin lesions in dogs and their virulence markers like siet gene will provide important data for clinical practice to manage the disease more effectively by means of treatment and prevention.Key words: Canine pyoderma, exfoliative toxin, siet gene, Staphylococcus intermedius. Piyodermalı köpeklerden izole edilen Staphylococcus intermedius'larda eksfoliatif toksin varlığının moleküler olarak belirlenmesi

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Population Diversity of Staphylococcus intermedius Isolates from Various Host Species: Typing by 16S-23S Intergenic Ribosomal DNA Spacer Polymorphism Analysis

Cited by 36 publications

References 24 publications

Reclassification of Phenotypically Identified Staphylococcus intermedius Strains

Reclassification of Phenotypically Identified Staphylococcus intermedius Strains

Methicillin-ResistantStaphylococcus pseudintermediusin a Veterinary Teaching Hospital

Molecular detection of exfoliative toxin in Staphylococcus intermedius isolates from dogs with pyoderma

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