The use of whole‐cell protein profile analysis by SDS‐PAGE as an accurate tool to identify species and subspecies of coagulase‐negative staphylococci

Santos, Odelta dos; Resende, Mariah Costa Carvalho de; Mello, Ariéle Lima De; Frazzon, Ana Paula Guedes; d’Azevedo, Pedro Alves

doi:10.1111/j.1600-0463.2011.02809.x

Cited by 7 publications

(9 citation statements)

References 26 publications

(65 reference statements)

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“…A significant amount of literature already exists discussing the uses of SDS-PAGE within the medical arena. For example, use of the technique to identify pathogenic bacteria to species is discussed by Emerson et al 25 and Kersters et al, 26 and its potential use as a screening tool to detect human bacterial pathogens is discussed by Santos et al 27…”

Section: Discussionmentioning

confidence: 99%

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Using SDS–PAGE gel fingerprinting to identify soft‐bodied wood‐boring insect larvae to species

O’Neill¹,

Denos²,

Reed³

2017

Pest Management Science

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The experience gained in undertaking this study gives us confidence that we will be able to develop a field deployable system in the medium term. We believe that as a high-throughput identification tool, this technology is superior to competitor technologies (e.g. fingerprinting of imaged DNA gels) in terms of speed, cost and ease of use; and therefore, is suitable for low-cost deployment in the field. © 2017 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

“…For example, use of the technique to identify pathogenic bacteria to species is discussed by Emerson et al . and Kersters et al ., and its potential use as a screening tool to detect human bacterial pathogens is discussed by Santos et al …”

Section: Discussionmentioning

confidence: 99%

Using SDS–PAGE gel fingerprinting to identify soft‐bodied wood‐boring insect larvae to species

O’Neill¹,

Denos²,

Reed³

2017

Pest Management Science

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“…Furthermore, the number 49 strain was very close to reference strain in both dendrograms. Similar studies have shown that the whole-cell and extracellular protein profiles can be useful in the characterization and the discrimination of S. aureus strains isolated from various sources [10,[22][23][24][25][26]. Previous studies proposed that whole-cell and extracellular protein profiles were sufficient for the differentiation at species level, but the differences were inadequate at the strain level [10,12].…”

Section: Resultsmentioning

confidence: 89%

Identification and Protein Fingerprinting of Staphylococcus aureus Species Isolated from Tooth Decay

Çine

Berber

Avşar

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Staphylococcus aureus is the most prevalent human pathogenic microorganism worldwide. In the present study, a total of 57 S. aureus strains, including 54 clinical isolates obtained from dental hospitals and three reference strains were characterized by phenotypic characteristics and SDS-PAGE protein profiling. Out of the 132 clinical specimens, 54 S. aureus strains were isolated and identified according to their morphological and physiological characteristics. The strains were tested against 13 standard antibiotics by using disc diffusion procedure. Antibiogram results showed that the highest resistance among the isolates was determined against ampicillin (83.6 %), whereas they were susceptible to sulfamethoxazole/trimethoprim, cefoperazone/sulbactam, vancomycin and imipenem. In addition, multidrug resistance index values of 12 (22.2 %) of the isolates were higher than the reference value of 0.2. The numerical analysis of wholecell protein profiles of S. aureus strains revealed 2 major clusters at similarity levels of 57 % or above. Furthermore, the numerical analysis of extra-cellular protein profiles of S. aureus strains yielded 2 major clusters at similarity levels of 48 % or above. In conclusion, the present findings revealed that use of conventional methods with the numerical analysis of whole-cell and extra-cellular protein profiles might be useful for the more reliable identification of S. aureus strains isolated from tooth decay.

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“…SDS-PAGE method has a discriminatory resolution to the level of species and subspecies (Vandamme et al 1996). Finger printing of total protein using SDS-PAGE has been applied to differentiate strains of Xanthomonas (Vandamme et al 1996;Vauterin et al 1993), members of the genus Bacillus (Berber 2004;Berber & Yenidunya 2005), members of Staphylococci (Santos et al 2012), and the group of Lactic Acid Bacteria (Ghazi et al 2009;Hong et al 2014).…”

Section: Identification Of the Amylolytic Bacteria Producing Of Phb Bmentioning

confidence: 99%

“…High resolution polyacrylamide gel electrophoresis (PAGE) of proteins with computerized analysis of profiles provided an effective approach to the investigation of taxonomic relationships among many bacterial species (Berber 2004;Berber & Yenidunya 2005). Several investigators have used SDS-PAGE of soluble bacterial polypeptides data for numerical taxonomy and an effective approach to the investigation of taxonomic relationships among many bacterial species (Berber 2004;Berber & Yenidunya 2005;Ghazi et al 2009;Santos et al 2012;Sarkono et al 2016). Therefore, this study aimed to identify the three isolates of bacterial producing bioplastics from Southeast Sulawesi, Indonesia by polyphasic taxonomy approach, particularly based on the characters of phenotypic, whole cell protein profile and genotypic characters, so as to illustrate the similarities between bacterial and the identity of the local bacterial isolates can be unveiled.…”

Section: Introductionmentioning

confidence: 99%

Polyphasic Identification of Amylolytic Bacteria Producing Bioplastic Poly-β-hydroxybutyrate (PHB)

Yanti¹,

Sembiring²,

Margino³

et al. 2019

JSM

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The final goal of this study is to make a modern systematic-based inventory of amylolytic bacterial isolates producing of bioplastic Poly-β-hydroxybutyrate (PHB) from sago starch substrate. The identity of three local bacterial isolates was examined in this study, using a polyphasic approach. A data set based on phenotypic characteristics, namely morphological, physiological, biochemical and chemical character, namely whole cells protein profiles using SDS-PAGE method, together with phylogenetic studies based on 16S rRNA sequences was used to identified by polyphasic approach. Phenotypic characteristics of 3 local bacterial isolates and 4 reference strains to members of genus Bacillus was analyzed by numerical analysis using MVSP 3,1 program to determine the value of similarity. Based on the preliminary characterization of the profile matching method showed that the three isolates of bacteria producing PHB namely PSA10, PPK5 and PPK6 are members of the genus Bacillus. The results of numerical analysis based on phenotypic characteristic and chemical character of the three bacterial isolates producing PHB with reference strains showed that the PSA10 isolate bacterial identical with Bacillus megaterium, PPK5 isolate identical with Bacillus subtilis and PPK6 isolate identical with Bacillus cereus, and these results also support by the molecular phylogenetic analysis. Therefore, the polyphasic taxonomy is an effective approach to uncover the identity of the novel bacterial isolates. ABSTRAKMatlamat akhir kajian ini adalah untuk membuat inventori berasaskan sistematik moden daripada pencilan bakteria amilolisis yang menghasilkan bioplastik Poli-β-hidroksibutirat (PHB) daripada substrat kanji sagu. Identiti tiga pencilan bakteria tempatan telah diperiksa dalam kajian ini, menggunakan pendekatan polifasa. Satu set data berdasarkan ciriciri fenotip, iaitu sifat morfologi, fisiologi, biokimia dan kimia, iaitu profil protein sel keseluruhan menggunakan kaedah SDS-PAGE, bersama-sama dengan kajian filogenetik berdasarkan urutan 16S rRNA digunakan untuk mengenal pasti dengan pendekatan polifasa. Ciri-ciri fenotip 3 pencilan bakteria tempatan dan 4 rujukan kepada ahli genus Bacillus dianalisis dengan analisis berangka menggunakan MVSP 3,1 program untuk menentukan nilai keserupaan. Berdasarkan pencirian permulaan kaedah pencocokan profil menunjukkan bahawa tiga pencilan bakteria yang menghasilkan PHB iaitu PSA10, PPK5 dan PPK6 adalah ahli genus Bacillus. Hasil analisis berangka berdasarkan pencirian fenotip dan sifat kimia ketiga pencilan bakteria yang menghasilkan PHB dengan strain rujukan menunjukkan bahawa PSA10 seiras dengan Bacillus megaterium, PPK5 seiras dengan Bacillus subtilis dan PPK6 seiras dengan Bacillus cereus, dan keputusan ini juga disokong oleh analisis filogenetik molekul. Oleh itu, taksonomi polifasa adalah pendekatan yang berkesan untuk mendedahkan identiti novel pencilan bakteria.

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The use of whole‐cell protein profile analysis by SDS‐PAGE as an accurate tool to identify species and subspecies of coagulase‐negative staphylococci

Cited by 7 publications

References 26 publications

Using SDS–PAGE gel fingerprinting to identify soft‐bodied wood‐boring insect larvae to species

Using SDS–PAGE gel fingerprinting to identify soft‐bodied wood‐boring insect larvae to species

Identification and Protein Fingerprinting of Staphylococcus aureus Species Isolated from Tooth Decay

Polyphasic Identification of Amylolytic Bacteria Producing Bioplastic Poly-β-hydroxybutyrate (PHB)

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