Using SDS–PAGE gel fingerprinting to identify soft‐bodied wood‐boring insect larvae to species

O’Neill, Mark A.; Denos, Mia; Reed, Daniel M.

doi:10.1002/ps.4766

Cited by 5 publications

(4 citation statements)

References 22 publications

(30 reference statements)

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“…SDS-PAGE gel ngerprints exhibited a distinct characteristic among three species. This was an agreement with the Mark A O'Neill et al, using SDS-PAGE gel ngerprint to identify soft-bodied wood-boring insect larvae to species [35].…”

Section: Discussionsupporting

confidence: 88%

Species Identification and Quantification of Bovine Bone Marrow from Non-Bovine Products using DNA and Protein Fingerprints

Guan

et al. 2021

Preprint

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Bovine bone marrow is traditionally regarded as a highly nutritious food that has been widely used as a medicinal and healthy food for several decades in China. A large number of adulterated and counterfeit bone marrows from pigs and donkeys have been used in place of bovine bone marrow in commercial products, which are almost identical morphologically to species. Therefore, we explored a feasibility of fingerprinting of multiplex PCR gels combined with imaged SDS-PAGE gels of proteins. Three pairs of specific primers for bovine, pig and donkey were designed according to the conserved sequence in mitochondrial cytochrome b. The optimal reaction conditions for triple PCR were optimized. A modified method was used to extract total proteins and SDS-PAGE gels of proteins were set up. A three-fold PCR detection assay was successfully established to identify three species of bovine, pig and donkey. Three primers have good specificity and high sensitivity. Additionally, the assay sensitivity test confirmed that the extracted DNA concentration was the lowest of bovine bone marrow at 0.1 pg. The assay also showed 100% specificity. The electrophoretogram of SDS-PAGE showed distinct electrophoresis profiles among three species. The established fingerprint of multiplex PCR DNA gels combined with imaged SDS-PAGE gels of proteins has strong specificity and can be quickly and accurately used for the identification of bovine bone marrow. Rapid authentication of bovine bone marrow and differentiation from non-bovine products can be achieved using an improved SDS alkali denaturation method, species-specific PCR assay combined with imaged SDS-PAGE gels of proteins.

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Section: Discussionsupporting

confidence: 88%

Species Identification and Quantification of Bovine Bone Marrow from Non-Bovine Products using DNA and Protein Fingerprints

Guan

et al. 2021

Preprint

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“…Taken together, a species-specific, PCR-based method to identify BBM is an essential research tool for improving the accuracy of detecting TCM compounds in medicinal herbs [29][30][31][32]. PCR-based methods are simple, highly specific, sensitive, and accurate, thereby complementing existing Chinese herbal medicine detection methods [33][34][35]. Next, we will adopt SDS-PAGE analysis of whole-cell proteins on gel fingerprints among three species, as previously described [36].…”

Section: Discussionmentioning

confidence: 99%

Species Identification of Bovine Bone Marrow from Nonbovine Products Using Multiplex PCR Technology

Guan

Sun

et al. 2022

Journal of Food Quality

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Bovine bone marrow is traditionally regarded as a highly nutritious food that has been widely used as a medicinal and health food for several decades in China. A large number of adulterated and counterfeit bone marrows from pigs and donkeys have been used in place of bovine bone marrow in commercial products, which are almost identical morphologically between species. Therefore, we explored the feasibility of multiplex PCR technology to differentiate bovine bone marrows from different domestic animals. Three pairs of specific primers for bovine, pig, and donkey were designed according to the conserved sequence in mitochondrial cytochrome b. A modified method was used to extract the genomic DNA from common domestic animals’ bone marrows. The optimal reaction conditions for triple PCR were optimized. A three-fold PCR detection assay was successfully established to identify three species of bovine, pig, and donkey. Three primers have good specificity and high sensitivity. Additionally, the assay sensitivity test confirmed that the extracted DNA concentration was the lowest in bovine bone marrow at 10°pg/μL. The assay also showed 100% specificity. Rapid authentication of bovine bone marrow and differentiation from nonbovine products can be achieved using an improved SDS alkali denaturation method and species-specific PCR assay. Both species-specific PCR methods described in this study can be potentially applied for the quality evaluation of functional food and drug resources.

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“…The concentration of the Rpf protein was determined by a modied Bradford Protein Assay Kit. 27,28 Finally, the puried protein was diluted with an equal volume of glycerol and stored at À20 C for further experiments.…”

Section: Rpf Protein Overexpression Purication and Electrophoretic Analysismentioning

confidence: 99%

Optimization of bacterial cytokine protein production by response surface methodology for environmental bioremediation

Xie

et al. 2021

RSC Adv.

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Using SDS–PAGE gel fingerprinting to identify soft‐bodied wood‐boring insect larvae to species

Cited by 5 publications

References 22 publications

Species Identification and Quantification of Bovine Bone Marrow from Non-Bovine Products using DNA and Protein Fingerprints

Species Identification and Quantification of Bovine Bone Marrow from Non-Bovine Products using DNA and Protein Fingerprints

Species Identification of Bovine Bone Marrow from Nonbovine Products Using Multiplex PCR Technology

Optimization of bacterial cytokine protein production by response surface methodology for environmental bioremediation

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