The Use of Vital Dyes in Ocular Surgery

Rodrigues, Eduardo B.; Costa, Elaine Fiod; Penha, Fernando M.; Melo, Gustavo Barreto; Bottós, Juliana Mantovani; Dib, Eduardo; Furlani, Bruno; Lima, Verônica Castro; Maia, Maurício; Meyer, Carsten H.; Höfling-Lima, Ana Luísa; Farah, Michel Eid

doi:10.1016/j.survophthal.2009.04.011

Cited by 110 publications

(127 citation statements)

References 386 publications

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“…Principal among the techniques used to study the corneal endothelium are specular microscopy and scanning electron microscopy (SEM) (PIGATTO et al, 2005a, PIGATTO et al, 2005bMcCAREY et al, 2008). Aside from these techniques, the analysis of the corneal endothelium using optical microscopy after cell staining with alizarin red has also been used (RODRIGUES et al, 2009 morphometric and morphological endothelial cell studies provided reference information for the investigation of clinical diseases, such as uveitis, glaucoma, and the effects of aging on different regions of the cornea. Furthermore, the only way to treat horses with cataract is to surgically remove the opacified lens (MILLICHAMP & DZIEZC, 2000;HARDMAN et al, 2001;FIFE et al, 2006;HARRINGTON et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Morphology of endothelial cells from different regions of the equine cornea

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Morphology of endothelial cells from different regions of the equine cornea

et al. 2016

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“…Unsuccessful CCC increases the risk of posterior capsule rupture, vitreous loss, nucleus drop and intraocular lens displacement. Rodrigues and collaborators tested the anterior lens stain capacity of the brilliant blue dye in some concentrations and concluded that the concentration of 0.05% was enough to stain the anterior swine lens capsule homogenously and to promote the observation of the flap portion (tissue being incised) during CCC, although its effects on corneal endothelium are not completely known (Rodrigues 2009). …”

Section: Discussionmentioning

confidence: 99%

Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study

et al. 2016

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ABSTRACT.-Terzariol M., Hünning P.S., Brambatti G., Albuquerque L., Neumann C. & Pigatto J.A. T. 2016 The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue ® ) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.INDEX TERMS: Eye, brilliant blue 0.05%, toxicity, corneal endothelium, swine.

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“…RB staining is widely used to diagnose ocular surface disorders (Rodrigues et al 2009;Murube 2014). It stains desquamated ocular epithelial cells, dead or degenerated cells, but not healthy epithelial cells (Mittal et al 2012).…”

Section: Rose Bengal Stainingmentioning

confidence: 99%

Non-Invasive Techniques for Detection and Diagnosis of Oral Potentially Malignant Disorders

Liu

Zhao

Zeng

et al. 2016

Tohoku J. Exp. Med.

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Oral squamous cell carcinoma (OSCC) is the most common oral and maxillofacial malignancy, and its morbidity and mortality rates are still high in most countries. Oral potentially malignant disorders (PMDs) are used to refer to a heterogeneous group of conditions that are characterized by increased risk for malignant transformation to OSCC. Currently identified oral PMDs include leukoplakia, erythroplakia, palatal lesions associated with reverse smoking, oral lichen planus, oral submucous fibrosis, actinic keratosis, and discoid lupus erythematosus. The early detection and diagnosis of these lesions are important for cancer prevention and disease management. In recent years, there has been a growing and persistent demand for new non-invasive, practical diagnostic techniques that might facilitate the early detection of oral PMDs. The non-invasive detection techniques evaluated in this review are divided into four categories: vital staining with a solution that can be used as a mouth rinse or applied onto a suspected area of the mouth, light-based detection systems, optical diagnostic technologies that employ returned optical signals to reflect structural and morphological changes within tissues, and salivary biomarkers. Most of these techniques have shown great potential for screening and monitoring oral PMDs. In this review article, the authors critically assess these non-invasive detection techniques for oral PMDs. We also provide a summary of the sensitivity and specificity of each technique in detecting oral PMDs and oral cancer, as well as their advantages, disadvantages, clinical applications, and indications.

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The Use of Vital Dyes in Ocular Surgery

Cited by 110 publications

References 386 publications

Morphology of endothelial cells from different regions of the equine cornea

Morphology of endothelial cells from different regions of the equine cornea

Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study

Non-Invasive Techniques for Detection and Diagnosis of Oral Potentially Malignant Disorders

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