RESUMORelata-se um caso de pitiose intestinal em um Husky Siberiano, de um ano de idade, macho atendido em um Hospital Veterinário Escola com sinais de obstrução intestinal. Ao exame físico, notou-se massa cilíndrica na região abdominal cranial, posteriormente confirmada por meio de radiografia e ultrassonografia. Durante a laparotomia exploratória, constatou-se massa extraluminal envolvendo o jejuno e alterações na parede do órgão. Realizou-se a ressecção da porção afetada do intestino e, posteriormente, anastomose. O exame histológico do tecido evidenciou inflamação piogranulomatosa acentuada. Na coloração de prata metenamina de Grocott, hifas septadas foram observadas. O diagnóstico de infecção por Pythium insidiosum foi confirmado por meio da imunoistoquímica. Após a cirurgia, o animal restabeleceu a defecação; no 30° dia pós-cirúrgico, foi relatada ainda presença de diarreia. O tratamento com itraconazol e terbinafina foi instituído durante 60 dias. Após dois anos do procedimento cirúrgico e do tratamento com antifúngicos orais, o cão não apresentou recidiva. (Cock et al., 1987;Graham et al., 2000; Rech et al., 2004;Rodrigues et al., 2006) , 2006). Somente o P. insisdiosum acomete mamíferos, entre os quais equinos, caninos, felinos, bovinos e humanos. Nas espécies não domésticas, já foi citado em um urso cativo, um jaguar e um camelo (Camus et al., 2004;Wellehan et al., 2004). A doença ocorre principalmente em regiões de clima tropical, subtropical e temperado, e já foi relatada no continente americano, alguns países europeus,

ABSTRACT.-Terzariol M., Hünning P.S., Brambatti G., Albuquerque L., Neumann C. & Pigatto J.A. T. 2016 The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue ® ) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.INDEX TERMS: Eye, brilliant blue 0.05%, toxicity, corneal endothelium, swine.

RESUMO Mensuração da pressão intraocular em ovinos, usando tonômetro de aplanaçãoO objetivo deste estudo foi avaliar e estabelecer os valores de pressão intraocular (PIO) média, em ovinos adultos saudáveis, usando a tonometria de aplanação. Foram utilizados no estudo 25 ovinos saudáveis (Ovis aries), da raça Texel, machos ou fêmeas de três anos de idade. Foram realizados exames oftálmicos, incluindo: avaliação dos reflexos pupilares, teste lacrimal de Schirmer, avaliação com lâmpada de fenda e coloração com fluoresceína, em ambos os olhos de todos os animais. Para a realização dos testes oftálmicos, os animais foram contidos pelo mesmo auxiliar, sem pressão na região jugular e as pálpebras foram delicadamente abertas. A PIO foi aferida, utilizando-se o tonômetro de aplanação Tonopen XL, tendo sido todos os exames realizados pelo mesmo examinador. Foram realizadas três avalia- The purpose of this study was to evaluate and establish the mean values of IOP in healthy adult sheep using an applanation tonometer. Information on age, sex, and breed was obtained for all animals included in this study. Twenty five healthy sheep (Ovis aries), of the same breed (Texel), male or female, with three years of age, received an ophthalmic examination in both eyes, including pupillary reflexes, Schirmer tear test, slit lamp biomicroscopy, and fluorescein staining. For all ophthalmic testing, animals were gently physically restrained, with no pressure in the jugular area and the eyelids were carefully open. IOP was measured by applanation tonometry (Tonopen XL). The same examiner performed the tonometry; measurements were taken three times for each eye, and their average was recorded as the IOP of the animal. Statistical analysis was performed using paired t-test and values of P < 0.05 were considered significant. The mean intraocular pressure in the whole group of 50 eyes was of 16.36 +/-2.19 mm Hg. The mean (SD) IOP in the right eye was of 15.96 +/-2.02 mm Hg, while the mean (SD) IOP in the left eye was of 16.76 +/-2.32 mm Hg. Significant differences in IOP were not found between right and left eyes. The applanation tonometer was adequate for measuring the intraocular pressure in sheep. Reference data will assist in diagnosing testing for ophthalmic disease in sheep, as 1 well as promote further studies in this area.

La deficiencia de piruvato quinasa (PK) es un desorden hemolítico autosómico recesivo descrito en perros y gatos. La piruvato quinasa es una de las enzimas regulatorias esenciales de la glicólisis anaeróbica, la deficiencia de esta enzima causa una destrucción prematura de los eritrocitos. El presente es un estudio de caso y relata los hallazgos clínicos y paraclínicos en un perro brasileño de la raza West Highland White Terrier (WHWT) con historia de debilidad e intolerancia al ejercicio. El paciente presentaba mucosas pálidas, anemia hemolítica bastante regenerativa y osteoclerosis. La deficiencia de PK fue confirmada a través de una prueba de ADN raza específica para la inserción 6bp en el extremo 3' del exón 10 de la secuencia del gen de la piruvato quinasa eritrocitaria (R-PK) como fue descrito. Al perro se le practicó eutanasia a los 20 meses de edad debido al deterioro de su estado clínico, el cual incluyó anemia e incompatibilidad sanguínea. En otros casos descritos en perros de la raza WHWT con esta deficiencia, existen relatos hasta de nueve años de sobrevivencia. Los defectos hereditarios deben ser objeto de diagnóstico diferencial importante en casos de anemias hemolíticas crónicas en animales jóvenes después de la exclusión diagnóstica de disturbios inmunomediados y causas infecciosas. Adicionalmente, perros de razas puras para las cuales la prueba de ADN está disponible para enfermedades hereditarias deben ser evaluados antes de la edad reproductiva para limitar la diseminación del alelo mutante y la generación futura de animales deficientes.

No abstract

Background:The Shaker Dog Syndrome manifests itself as generalized tremors that usually affect the head and body of the patient. The tremors increase with movement and decrease at rest, and it may cease during sleep. The disorder develops mostly in 1-year to 5-year-old animals weighing below 15 kg. The diagnosis is based on the exclusion of other possible causes of tremors and a positive response to treatment with corticosteroids. The treatment consists of immunosuppressant doses of corticosteroids and it can be associated to decreasing diazepam doses during 8 to 12 weeks. Literature data are very rare and cannot be found in Brazil. Consequently, this study aimed at describing a Shaker Dog Syndrome case that is responsive to corticosteroids. Case: A 2-year-old mongrel female dog, weighting 7.5 kg, was referred to the Hospital de Clínicas Veterinárias da Universidade Federal do Rio Grande do Sul, presenting generalized tremors for 5 days, which would cease during sleep. In the neurological examination, the cranial nerve exam and the test for postural reactions were considered normal. The hematological and biochemical profile did not show alterations. The cervical spine radiography did not display bone alterations. A density of 1.016 was observed at the physical examination of the cerebrospinal fluid. The chemical examination revealed a glucose level of 100 mg/dL, negative bilirubin, negative ketones, absence of occult blood, pH 8.0, presence of protein traces, normal urobilinogen, negative reactive protein C (PCR), normal creatine kinase (CK) and aspartate aminotransferase (AST). The result of Pandy's test was negative. Oral treatment with prednisone at a dose of 1.5 mg.kg -1 BID and diazepam at a dose of 0.5 mg.kg -1 BID was instituted, this latter during 4 days. The corticoid dose was maintained until total disappearance of the tremors, which occurred within 24 days. Afterwards, a gradual reduction of 50% of the dose was made every 7 days, during 4 weeks. Discussion: During anamnesis, tremors induced by trauma and medication were ruled out. Myelin abnormalities were also improbable, as they cause congenital tremors and do not quickly respond to corticosteroids. A colorless aspect and absence of occult blood and bilirubin was observed in the LCR analysis. The absence of turbidity and the presence of protein traces in this sample demonstrate normality (38.7 mg/dL ± 15.49). Chemical and physical exams did not demonstrate alterations -pH 8.0 (8.30 ± 0.34) and a density of 1.016 (1007 ± 1.83), respectively. The glucose concentration in the cerebrospinal fluid was not reduced. The results obtained from Pandy's and reactive protein C tests were negative. The analysis of AST and CK enzymes was normal. Since the values obtained from enzymatic and protein evaluations of the cerebrospinal fluid were normal, they suggest an absence of infectious and degenerative processes. The immunosuppressant prednisone dose recommended by literature is variable; thus, the smallest corticosteroid dose indicated was selected, which is 1.5 ...

Infestação mista por Lynxacarus radovskyi e Felicola subrostratus em um gato na região de Porto Alegre, RS, Brasil. Acta Scientiae Veterinariae. 37(3): 301-305.A linxacariose é uma doença incomum que acomete felinos, principalmente em regiões tropicais, porém há registros também em regiões subtropicais. No Brasil, foi descrita pela primeira vez em 1986, e atualmente existem relatos em todas as regiões, excluindo o centro-oeste do país. No Rio Grande do Sul, somente alguns casos foram relatados no ano de 1997 em felinos das raças persa e exótico. Esta doença é causada pela infestação do ácaro Lynxacarus radovskyi. Algumas vezes, esta infestação pode estar associada a outras parasitoses externas, na qual a mais comum é a pediculose por Felicola subrostratus, parasita de distribuição mundial. Geralmente, a transmissão é dada por contato direto ou por fômites, mas não é considerada uma doença de alto contágio. Os animais infestados apresentam variado grau de prurido, alopecia, disqueratinização, pelagem opaca e pelos aparentemente com sujidades, tradicionalmente com o aspecto "sal e pimenta". Algumas vezes, a manifestação clínica da doença pode ser confundida com outras dermatopatias, não sendo corretamente diagnosticada. O diagnóstico é dado pela visualização direta do ácaro e por microscopia óptica. O objetivo do relato é descrever um caso de infestação ABSTRACT Lynxacariosis is an uncommon parasitic disease in felines recorded in tropical, and sometimes subtropical, regions of varied countries. In Brazil lynxacariosis was first described in 1986, in Rio de Janeiro, and currently there are reports in all regions of the country, excluding the central-west region. In Rio Grande do Sul, only a few cases were reported in 1997 in Persian Exotic cats. The disease is caused by the infestation of the mite Lynxacarus radovskyi. Sometimes this infestation can be mixed with other external parasitoses, most commonly with the pediculosis caused by Felicola subrostratus, a parasite distributed worldwide. Usually the transmission occurs by direct contact or by fomites, but it is not considered as a highly transmissible disease. The infested animals present variable degree of pruritus, alopecia, scalling, and a "salt and pepper" appearance to the dull and dirty coat. Sometimes the clinical manifestation of the disease can be confused with other dermatitis, not being correctly diagnosed. The diagnosis is confirmed by the direct visualization of the mite and by optic microscopy. The objective of this article is to report a case of infestation by L. radoviskyi associated with F. subrostratus in a mixed cat, attended

The purpose of this study was to verify the effect of the upper lip twitch restraint on intraocular pressure (IOP) of healthy horses. In this study, forty five Criollo horses, aged between two to